Robust Stereoscopic Crosstalk Prediction

We propose a new metric to predict perceived crosstalk using the original images rather than both the original and ghosted images. The proposed metrics are based on color information. First, we extract a disparity map, a color difference map, and a color contrast map from original image pairs. Then, we use those maps to construct two new metrics (Vdispc and Vdlogc). Metric Vdispc considers the effect of the disparity map and the color difference map, while Vdlogc addresses the influence of the color contrast map. The prediction performance is evaluated using various types of stereoscopic crosstalk images. By incorporating Vdispc and Vdlogc, the new metric Vpdlc is proposed to achieve a higher correlation with the perceived subject crosstalk scores. Experimental results show that the new metrics achieve better performance than previous methods, which indicate that color information is one key factor for crosstalk visible prediction. Furthermore, we construct a new data set to evaluate our new metrics

Surveillance of Norovirus contamination on scallops sold in Beijing

Objective To monitor the contamination of Norovirus and Rotavirus in scallops sold in Beijing and analyze their genetic characteristics. Methods A total of 72 scallops were collected during November 2014 and October 2015. The digestive tissues of scallops were excised, Norovirus was enriched using the polyethylene glycol (PEG) 8000 precipitation method. The virus RNA were extracted and tested for Norovirus and Rotavirus using the real-time reverse transcription-polymerase chain reaction (RT-PCR). The capsid gene of Norovirus was amplified by the semi-nested RT-PCR. PCR products were sequenced directly. Sequence alignment was performed using BioEdit 7.0.9.0 software and the phylogenetic tree was constructed by MEGA 6.06 software. Results Among 72 scallops, Rotavirus was detected negative, and the detection rate of Norovirus was 27.8% (20/72), including 16 strains of genogroup GⅡ, 2 strains of genogroup GⅠ, and 2 strains of mixed genogroups GⅠ and GⅡ. The highest detection rate of Norovirus (61.1%, 11/18) was found in winter, and none was detected in summer. A total of 8 strains were sequenced successfully and identified as GⅡ.17 genotype. These strains all belonged to the Cluster Ⅲb branch of GⅡ.17 genotype, among which 6 strains shared 100.0% sequence similarity with strains detected in Chinese people in 2015, in Japanese people in 2016 and 2017, in water samples in South Korea in 2017 and 2018, and in oysters in Japan in 2015. Conclusion Norovirus contamination existed in some scallops sold in Beijing, eating uncooked scallops would indicate a risk of viral acute gastroenteritis

Collapsin response mediator protein 5-associated optic neuropathy: clinical characteristics, radiologic clues, and outcomes

ObjectiveCollapsin response mediator protein 5-associated optic neuropathy (CRMP5-ON) is a rare entity of autoimmune optic neuropathy. This study aimed to review the neuro-ophthalmic findings and outcomes in a series of patients with CRMP5-ON to further characterize its clinical phenotype, radiologic clues, and outcomes.MethodsThis was a retrospective case series and a single-center medical chart review of all patients with CRPM5-seropositive ON at the Department of Neurology, Beijing Tongren Hospital, from December 1, 2020, to March 31, 2023. The main outcome measures were neuro-ophthalmic manifestations, radiologic characteristics, and clinical outcomes of CRMP5-ON; coexisting neural autoantibody, paraneoplastic associations, and the impact of immunosuppressant therapy.ResultsFive patients were identified. Four (80%) were female, and the average age at onset was 59.4 years (range 53–69 years), with an average follow-up of 15.3 months (range 1.4–28.7 months). The average best-corrected visual acuity (BCVA) at nadir was 20/120 (range 20/20 to count fingers). Seven of ten affected eyes (70%) showed diffuse defects of the central field. Painless bilateral involvement and optic disk edema occurred in 100% of patients, combined with vitritis, uveitis, or retinitis in four (80%). Four patients (80%) had MRI abnormalities along the optic nerve (one patient with optic nerve enhancement and three patients had optic nerve sheath enhancement or peribulbar fat enhancement). Three patients (60%) had optic neuropathy with other neurologic symptoms. Four patients (80%) had confirmed cancer (two were small-cell lung carcinoma, one was papillary thyroid carcinoma and another was thymoma and invasive pulmonary adenocarcinoma). All cancers were identified after the presentation of the optic neuropathy. The intervention included IVIG, IVMP, surgery and chemotherapy. The average BCVA at the last follow-up was 20/50 (range 20/20 to count fingers). Three patients had surgery during the initial hospitalization, and were stable during the follow-up. Among two patients who received IVMP, both had improvement after treatment, although one patient had worsening non-ocular neurologic symptoms during the steroid taper.ConclusionCRMP5-ON presented with optic disc edema, often bilateral involved and combined with vitreitis, retinitis, or uveitis. CRMP5-ON can present with MRI optic nerve or perineural optic nerve enhancement, especially in the optic nerve sheath. CRMP5-ON is closely related to paraneoplastic neurologic syndrome. Cancer screening and intervention are crucial to prognosis

Instant Stippling on 3D Scenes

In this paper, we present a novel real‐time approach to generate high‐quality stippling on 3D scenes. The proposed method is built on a precomputed 2D sample sequence called incremental Voronoi set with blue‐noise properties. A rejection sampling scheme is then applied to achieve tone reproduction, by thresholding the sample indices proportional to the inverse target tonal value to produce a suitable stipple density. Our approach is suitable for stippling large‐scale or even dynamic scenes because the thresholding of individual stipples is trivially parallelizable. In addition, the static nature of the underlying sequence benefits the frame‐to‐frame coherence of the stippling. Finally, we propose an extension that supports stipples of varying sizes and tonal values, leading to smoother spatial and temporal transitions. Experimental results reveal that the temporal coherence and real‐time performance of our approach are superior to those of previous approaches.</p

Ultralow Laser Power Three-Dimensional Superresolution Microscopy Based on Digitally Enhanced STED

The resolution of optical microscopes is limited by the optical diffraction limit; in particular, the axial resolution is much lower than the lateral resolution, which hinders the clear distinction of the three-dimensional (3D) structure of cells. Although stimulated emission depletion (STED) superresolution microscopy can break through the optical diffraction limit to achieve 3D superresolution imaging, traditional 3D STED requires high depletion laser power to acquire high-resolution images, which can cause irreversible light damage to biological samples and probes. Therefore, we developed an ultralow laser power 3D STED superresolution imaging method. On the basis of this method, we obtained lateral and axial resolutions of 71 nm and 144 nm, respectively, in fixed cells with 0.65 mW depletion laser power. This method will have broad application prospects in 3D superresolution imaging of living cells

Immunosuppressive Therapy of Chinese Isolated Non-MS Idiopathic Optic Neuritis

"Immunosuppressive therapy is recommended for preventing the relapse of neuromyelitis optica(NMO) and NMO-spectrum diseases, but very few studies focused on isolated Non-MS idiopathic optic neuritis(Non-MS-ION).

Additional file 1: Table S1. of Molecular network-based analysis of the mechanism of liver injury induced by volatile oils from Artemisiae argyi folium

DILI-related genes searched in PubChem. In the GenBank database 338 genes were identified involved in DILI. (DOCX 53 kb

Prevalence and genotypes of group A rotavirus among outpatient children under five years old with diarrhea in Beijing, China, 2011–2016

Abstract Background Rotavirus is a leading cause of severe diarrheal disease, and one of the common causes of death in children aged under five years old. The dominant epidemic strains may change in different years in the same area. In order to provide evidence for rotavirus epidemic control and inform vaccine development, we analyzed epidemiological patterns and genetic characteristics of rotavirus in Beijing during 2011–2016. Methods Stool specimens of outpatient children under five years old were collected from three children’s hospitals on a weekly basis. Group A rotavirus antigens were detected using enzyme-linked immunosorbent assay (ELISA) kit. The partial VP4 genes and VP7 genes of rotavirus were both amplified and sequenced. Genotyping and phylogenetic analyses were performed. Logistic regression and Chi-square tests were performed to determine differences across age groups, districts and years in rotavirus prevalence and genotype distribution. Results A total of 3668 stool specimens from children with acute diarrhea identified through hospital-based surveillance were collected from 2011 to 2016 in Beijing. A total of 762 (20.8%) specimens tested positive for rotavirus. The rotavirus-positive rate was highest among the 1–2 years old age group (29.0%, 310/1070). November, December and January were the highest rotavirus-positive rate months each year. G9 was the most common G genotype (64.4%, 461/716), and P [8] was the most common P genotype (87.0%, 623/716) among the 716 rotavirus-positive specimens. G9P [8], G3P [8] and G2P [4] were the most common strains. The rotavirus-positive rates of samples in 2012 and 2013 were higher than that in 2011, and the dominant genotype changed from G3P [8] to G9P [8] in 2012 and 2013. VP7 gene sequences of G9 strains in this study clustered into two main lineages. Most of the G9 strains exhibited the highest nucleotide similarity (99.1%~ 100.0%) to the strain found in Japan (MI1128). VP4 gene sequences of P [8] strains were almost P[8]b. Conclusions Rotavirus accounted for more than one fifth of childhood diarrhea in Beijing during the study period. Targeted measures such as immunization with effective rotavirus vaccines should be carried out to reduce the morbidity and mortality due to rotavirus