Pathogen Response Genes Mediate Caenorhabditis elegans Innate Immunity

Innate immunity is crucial in the response and defense against pathogens for invertebrates and vertebrates alike. The soil nematode Caenorhabditis elegans is a useful model to study the eukaryotic innate immune response to microbial pathogenesis. Prior research indicates that the protein receptor FSHR-1 plays an important role in the innate recognition of intestinal infection due to pathogen consumption. Determining what genes are controlled by FSHR-1 may uncover an unknown pathway that could increase not only the comprehension of the C. elegans immune system but also innate immunity generally. To characterize the function of FSHR-1, four candidate pathogen response genes that appear to be regulated by FSHR-1 were evaluated in worms infected with Pseudomonas aeruginosa. Although intestine specific RNA interference of these four genes did not show immunity phenotypes, quantitative PCR suggests that FSHR-1 regulates the basal and/or infection-induced expression of three of the four genes. To explore this FSHR-1-dependent transcriptional induction, fluorescent transgenic reporters were constructed for the three candidate FSHR-1 target genes. The spatial expression of one putative pathogen response gene was characterized in transgenic worms under both control and pathogenic conditions. RNA interference was performed to assess the FSHR-1 dependency of this expression pattern

Determining the Territorial Scope of State Law in Interstate and International Conflicts: Comments on the Draft Restatement (Third) and on the Role of Party Autonomy

Analyzing a conflict of laws requires thinking both about the scope of potentially applicable law and about priority, or choice, among potentially applicable laws. The Restatement (Second) of Conflict of Laws, published in 1971, contains little guidance on how, or in what order, courts are to address these two inquiries. The draft Restatement (Third), in contrast, differentiates clearly the respective roles of the two analytical elements. It characterizes the resolution of a choice-of-law question as a two-step process. First, the scope of the relevant states’ internal laws must be determined, in order to ascertain which states’ laws might be used as a rule of decision. Second, if more than one state’s law might be used as a rule of decision, and those laws conflict, it must be decided which law is given priority. The draft defines “internal law” to include restrictions on the geographic scope of the law. However, there are two questions the draft does not answer clearly. First, is the definition of internal law meant to include only express restrictions on scope? Second, absent explicit indications of legislative intent, how is the scope of a law to be determined? In particular, should courts employ a presumption against the extraterritorial application of state law? This article begins by analyzing the role of the presumption against extraterritoriality in supplying implied restrictions on the scope of law. It considers the role of the presumption in both international and interstate conflicts of laws, and argues that the Restatement (Third) should differentiate clearly between those two contexts. It then turns to the question whether geographic scope restrictions should properly be considered part of a state’s internal law. The paper analyzes that question through the lens of a common problem: a contract dispute involving a transaction or event that falls outside the scope of the law chosen by the parties to govern their agreement. On the basis of that analysis, it concludes that forthcoming sections will need to address the implications of the draft’s categorical treatment of legislative scope

A Review of Ankylosing Spondylitis

Ankylosing spondylitis (AS) is a systemic autoimmune disorder that induces ankylosis of the spine (fusion of the vertebrae at their various joints) and inflammatory arthritis of peripheral joints among other symptoms. Overexpression of cytokines, the presence of genetic mutations not exclusive to the human leucocyte antigen (HLA)-B27 region, and environmental factors all have large roles in the progressive development of AS. Although a definitive pathology continues to be sought after, researchers believe the adaptive immune system in AS patients attacks fibrocartilaginous entheses (supportive connective tissue between bone and attached structures like tendon, ligament, and fascia). AS markedly reduces proper systemic functioning in several areas of human physiology, including the musculoskeletal, cardiovascular, neurological, psychiatric, and reproductive systems in both genders. A diagnosis for this disease requires the presentation of several qualifying symptoms, namely chronic inflammatory back pain, peripheral joint arthritis, enthesitis (inflammation of the enthesis not associated with a joint), uveitis (inflammation of the uvea or inner eye layer), and positive response to non-steroidal anti-inflammatory drugs (NSAIDs), with radiological support through x-ray or magnetic resonance imaging (MRI). Upon an AS diagnosis, patients should engage in healthy lifestyle changes, non-impact exercise, and taking NSAIDs as the first pharmacological treatment. Symptoms unresolved by NSAIDs are then treated with disease modifying antirheumatic drugs (DMARDs) and/or biologic medications like a monoclonal TNFα antibody to prevent further disease progression. Continued research to understand the association between AS and interleukin (IL)-17/IL-23 is needed for development of additional biologic treatments

Emergent Landscape: Urban Shadow Space, Illuminated

This study defines a new approach to the transformation of unmaintained land within cities, or urban shadow space. Although urban shadow space can offer a place of free expression for the community and spontaneous vegetative growth within a city, it is often dismissed as blighted land by public authority. This study maximizes existing opportunities of these spaces, illuminating a realm of the city that is currently dark to the public eye. A proposed set of guidelines is utilized in the creation of three alternative designs that illustrate the emergent landscape, a sensitively designed, evolving landscape that encourages user interaction with the site. These guidelines and the results of their application are intended to assist design professionals who wish to move beyond the typical “clean and green” strategy currently employed by many municipalities to embrace a site’s existing characteristics

Basic Human Factors Task Data Relationships in Aerospace System Design and Development Final Report, Aug. - Dec. 1965

Basic human factors task data relationships in aerospace system design and developmen

The Association Between Advanced Maternal Age and Short Interpregnancy Intervals on Preterm Labor

Background: Preterm birth is the leading cause of neonatal and infant mortality and has become a major health concern due to the increasing rates of infant deaths in the United States (WHO, 2017). Studying maternal risk factors for preterm labor provides insight to this obscure condition and can assist in the identification of high risk women, as well as facilitate appropriate pregnancy planning. Purpose: Although research can be found on interpregnancy intervals and maternal age as independent risk factors for premature labor, gaps exist within the relation of these variables. This study was done to investigate whether there is a significant risk association between advanced maternal age (35 years and older) and short interpregnancy intervals on premature labor, that deems transferring out of a low risk birthing center to a more advanced hospitalized setting. Methodology: De-identified data regarding obstetric history, medical history, and pregnancy morbidity was abstracted from women who delivered at Baby + Co., a birthing center in Nashville, Tennessee, between the years of 2015 and 2018. The population set included 1001 women, 5 of which delivered preterm. Means and standard deviations for the two groups were calculated, and two sided t-tests and corresponding p-values were calculated. Result: There was no statistical significance regarding maternal age and preterm transfers (p-value of 0.762). However, there was a positive correlation between short interpregnancy intervals and preterm birth (p-value .007). Discussion: Due to the low risk population included in this study, there is a need for additional research conducted within a higher risk population set to determine the significance and interaction between advanced maternal age and short interpregnancy intervals on preterm labor

Got Caries? Breast milk and Early Childhood Caries?

Objective: To provide an analysis of the association between the longevity of breastfeeding and development of ECC. Determine the optimal time frame in which mothers should cease breastfeeding to reduce ECC development. Methods: Dr. Brickhouse, PubMed, Google Scholar and other scholarly databases were utilized to find current scientific evidence on the effects of breast milk on ECC. Relevant articles were summarized to write a review of literature. 16 articles published from 2015 to the present date were reviewed and cited. Results: From the studies, there is strong evidence to support breastfeeding beyond 12 months of age increases the prevalence of ECC. Furthermore, increased frequency and duration of breastfeeding leads to higher incidence of ECC. Conclusion: Findings indicate dental health care providers should recommend either ceasing breastfeeding at 12 months of age or provide ECC prevention education to caregivers. Further research is required to minimize data discrepancies between US and international countries.https://scholarscompass.vcu.edu/denh_student/1002/thumbnail.jp