Reconstitution of Kidney Side Population Cells after Ischemia-Reperfusion Injury by Self-Proliferation and Bone Marrow-Derived Cell Homing

The aim of this study was to examine the contribution of side population (SP) cells from kidney and bone marrow for reconstitution of kidney SP pools after ischemia-reperfusion injury (IRI). The SP and non-SP cells in kidneys following IRI were isolated and serially assessed by fluorescence-activated cell sorting. The apoptosis, proliferation, phenotype, and paracrine actions of SP cells were evaluated in vitro and in vivo. Results indicated that the SP cells from ischemic kidney were acutely depleted within one day following renal IRI and were progressively restored to baseline within 7 days after IRI, through both proliferation of remaining kidney SP cells and homing of bone marrow-derived cells to ischemic kidney. Either hypoxia or serum deprivation alone increased apoptosis of SP cells, and a combination of both further aggravated it. Furthermore, hypoxia in vivo and in vitro induced the increase in the secretion of vascular endothelial growth factor, insulin-like growth factor 1, hepatocyte growth factor, and stromal cell-derived factor-1α in kidney SP but not non-SP cells. In summary, these results suggest that following renal IRI, kidney SP cells are acutely depleted and then progressively restored to baseline levels by both self-proliferation and extrarenal source, that is, bone marrow-derived cell homing

Case report: Metagenomics next-generation sequencing in the diagnosis of septic shock due to Fusobacterium necrophorum in a 6-year-old child

Fusobacterium necrophorum (F. necrophorum) infection is rare in pediatrics. In addition, the detection time of F. necrophorum by blood culture is long, and the positive rate is low. Infection with F. necrophorum bacilli usually follows rapid disease progression, resulting in high mortality. In previous reports of F. necrophorum-related cases, the most dangerous moment of the disease occurred after the appearance of Lemierre’s syndrome. We report an atypical case of a 6-year-old female patient who developed septic shock within 24 h of admission due to F. necrophorum infection in the absence of Lemierre’s syndrome. F. necrophorum was identified in a blood sample by metagenomics next-generation sequencing (mNGS) but not by standard blood culture. The patient was finally cured and discharged after receiving timely and effective targeted anti-infection treatment. In the present case study, it was observed that the heightened virulence and invasiveness of F. necrophorum contribute significantly to its role as a primary pathogen in pediatric septic shock. This can precipitate hemodynamic instability and multiple organ failure, even in the absence of Lemierre’s syndrome. The use of mNGS can deeply and rapidly identify infectious pathogens, guide the use of targeted antibiotics, and greatly improve the survival rate of patients

Optimal planning for partially self-sufficient microgrid with limited annual electricity exchange with distribution grid

Existing research on on-grid microgrid planning is carried out with a free trading assumption and without considering the limitation of annual electricity exchange. Therefore, the existing planning and sizing scheme may be not viable for the application of partially self-sufficient microgrid (PSSMG) with a limited amount of electricity exchange. To address this issue, a new planning method for PSSMG is proposed in this paper considering the limited annual electricity exchanging amount (AEEA). The sizing model and energy management are linearized and simultaneously integrated into one model, which could be solved in polynomial time. In order to effectively reduce the number of variables of a full year horizon and to cope with the uncertainty both of DGs and loads, a data-driven method based on K-means algorithm is utilized to choose a set of typical days that are representative of historical data of one full year. Finally, the validity and effectiveness of the proposed model are verified by comparative numerical simulations, and the sensitivity of limited AEEA to the planning scheme is analyzed

Research on the Macro-Mesoscopic Response Mechanism of Multisphere Approximated Heteromorphic Tailing Particles

AbstractThe shape of tailing particles is essential factors of their macroscopic mechanical properties. Scholars have studied the effects of controllable factors, such as loading method, confining pressure, and strain rate, on the strength of tailing sand. However, research on the tailing particle structure and shape through laboratory tests has proved to be difficult due to the uncertain and discrete tailing particle distribution. Thus, the macro-mesoscopic response of heteromorphic tailing particles is rarely investigated. In this paper, the macro-mesoscopic response of heteromorphic tailing particles is studied using multisphere approximation, and numerical simulation of triaxial tests on the particles is conducted. Nonlinear evolution patterns of porosity, internal friction angle, and cohesion of heteromorphic tailing particles with the variation of angularity were investigated using the flexible boundary program developed in this study, which revealed the intrinsic relationship between the mesostructure evolution mechanism and the macroscopic engineering characteristics of heteromorphic tailing particles. The research results showed that (1) changes in angularity led to tailing particle rearrangements and, in turn, porosity changes. With increased angularity and confining pressure, particle sphericity decreased, and the deviatoric and peak stress increased accordingly. In the meantime, the softening was more significant as the peak stress was exceeded, while the cohesive force generally increased. (2) With fixed particle shape and angularity, the internal friction angle decreased slightly as the effective confining pressure increased. (3) In the shearing process, the simulated contact force chain evolution of tailing particles with different shapes was similar. The disordered contact force chains gradually undergo directional connection, i.e., the increased confining pressure reduced the number of free tailing particles and increased the number of stressed particles. (4) The triaxial stress-strain and peak stress in rigid boundary simulations under different confining pressures were slightly lower than those in the flexible boundary simulations. However, the difference was insignificant, indicating the good feasibility and reasonability of rigid boundary simulations for the macroscopic mechanical behaviors in triaxial tests. The research results could offer more direct insights into the macro-mesoscopic response and mechanical mechanisms of nonspherical particles and provide references for the simulation of tailings at the microscopic levels

RUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1

Here we presented that the expression of RUNX3 was significantly decreased in 75 cases of clear cell renal cell carcinoma (CCRCC) tissues (p<0.05). Enforced RUNX3 expression mediated 786-O cells to exhibit inhibition of growth, G1 cell-cycle arrest and metastasis in vitro, and to lost tumorigenicity in nude mouse model in vivo. RUNX3-induced growth suppression was found partially to regulate various proteins, including inhibition of cyclinD1, cyclinE, cdk2, cdk4 and p-Rb, but increase of p27Kip1, Rb and TIMP-1. Therefore, RUNX3 had the function of inhibiting the proliferative and metastatic abilities of CCRCC cells by regulating cyclins and TIMP1

Reneissance historiographical traditions in the works of 16th century English historians

Par Renesanses laikmeta sākumu Anglijā ir pieņemts uzskatīt Tjudoru dinastijas nākšanu pie varas 1485.gadā. Pateicoties humānisma vēsmām, periods atnesa ievērojamas pārmaiņas daudzās dzīves sfērās. Transformāciju piedzīvoja arī angļu vēsturnieku pārstāvētās historiogrāfiskās tradīcijas. Maģistra darba mērķis ir analizēt 16. gadsimta Anglijas vēsturnieku darbus ar nolūku konstatēt, kādā veidā tajos izpaudās Renesanses laikmetam raksturīgas vēstures izprašanas un rakstīšanas tendences. Darba rezultātā tika secināts, ka 16. gadsimta angļu historiogrāfijā nav izveidojusies spēcīga “humānistiskā” skola klasiskajā šī vārdā izpratnē. Ārējo faktoru simbioze lika vēsturniekiem pārvērtēt vēsturisko sacerējumu naratīvu savā individuālā veidā, saglabājot galvenās Renesanses historiogrāfijas pamatnostādnes, bet pārliekot akcentus uz sev svarīgu tēmu.It is generally considered that the Renaissance era in England began in 1485 with the Tudor dynasty seizing the crown. Humanist ideas brought broad changes to the 16th century England. During this period, English historiography also experienced significant transformation. The purpose of this Master's thesis is to analyze the works of the 16th century English historians and learn what significant trends of the Renaissance era historiography can be found. Analysis led to the conclusion that in the 16th century English historiography a strong Humanist school was never established. The symbiosis of external factors led the historians to approach their historical narrative in a more individual way, through variety of topics and accents, while also retaining the fundamental principles of the Renaissance era historiography

Ag∕Si(111)√3×√3 : Surface band splitting and the inequivalent triangle model

The atomic and electronic structures of the Ag∕Si(111)√3×√3 surface are currently under debate. By employing angle-resolved valence-band spectroscopy, the surface band dispersions around the K̅ point of the Ag∕Si(111)√3×√3 surface have been investigated in detail. Contrary to a recent study, we conclude that the S2 and S3 surface state bands do not show any detectable split at 100 K. Thus, photoemission spectra at both room temperature and 100 K show only a single peak at the K̅ point without any direct evidence of a split. Calculated band structures for the inequivalent triangle (IET) model show a gap at the K̅ point in contrast to the honeycomb-chain-trimer (HCT) model. We find, however, that there is no real contradiction between our photoemission data and the IET model provided the energy gap of the latter model is small as indicated by a recent calculation [ Phys. Rev. B 70 245431 (2004)]