6 research outputs found
<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Flavonoid profile and antioxidant activities of methanolic extract of <i>Hyparrhenia hirta</i> <span style="mso-bidi-font-style:italic">(L.) Stapf.</span></span>
208-215In this
study, we report isolation of flavonoids, viz.,
3-O-methylquercetin, tangeritin, luteolin-7-O-glucoside, luteolin,
apigenin-7-O-glucoside, apigenin-8-C-glucoside, luteolin-8-C-glucoside,
luteolin-6-C-glucoside, diosmetin and catechin from the methanolic extract of Hyparrhenia
hirta employing high performance liquid chromatography and liquid
chromatography-electrospray ionization-tandem mass spectrometry. The total
phenolic content of H. hirta extract was 105.58 ± 0.1 mg gallic acid
equivalents/g of plant extract while the total flavonoid content was 45.20 ±
0.2 mg quercetin equivalents/g of plant extract and the total condensed
tannin were 72.35 ± 0.7 mg catechin equivalents/g of plant extract by
reference to standard curve. The antioxidant activity was assayed through the
antioxidant capacity by phosphomolybdenum assay, the reducing power assay and
the radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl method. The
extract showed dose dependant activity in all the three assays.
</span
Acute oral toxicity study on Wistar rats fed microalgal protein hydrolysates from Bellerochea malleus
International audienceProtein hydrolysates and bioactive peptides from various protein sources have demonstrated their effectiveness for the prevention of illness and the improvement of symptoms from several diseases. In particular, the use of microalgae to generate bioactive peptides has received a growing interest because of their potential to be cultivated on non-arable land and high nutritional value. However, scant research is available on the toxicity of peptide-based preparations. The present study aims to evaluate the toxicity of microalgal protein hydrolysates (MPH) from one marine species of microalgae (Bellerochea malleus) to determine the feasibility of their use for functional food applications. Results showed that the oral administration of MPH at three doses (D1, 100 mg kgâ1 BW; D2, 400 mg kgâ1 BW; and D3, 2000 mg kgâ1 BW) to male Wistar rats did not induce any adverse effects or mortality up to13 days of treatment. Data analysis of relative organ weights and biochemical and hematologicalparameters did not show any significant differences between control and treated groups at the three doses investigated. Data from histopathological observations did not reveal any signs of major toxicity at the doses D1 and D2. However, mild signs of inflammation and necrosis were observed in the kidney of rats fed MPH at D3. All together, these results reveal the overall safety of MPH and provide new evidence for advocating their use for functional food or nutraceutical applications
Synthesis of Silver and Gold Nanoparticles from Rumex roseus Plant Extract and Their Application in Electrochemical Sensors
The room-temperature synthesis of silver (AgNPs) and gold (AuNPs) nanoparticles from aqueous solution of AgNO3 and HAuCl4 respectively, using Rumex roseus (RR) plant extract as a reducing agent, is reported here for the first time. The nanoparticles obtained were characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS). The formation of nanoparticles with spherical-shaped morphology was verified by TEM and confirmed by UV-Vis spectroscopy through the analysis of Ag and Au plasmon resonance peak and DLS measurements. New electrochemical sensors have been developed by employing the synthesized Ag and Au nanoparticles as modifiers of glassy carbon electrode (GCE) and screen-printed carbon electrode (SPCE), respectively. The AgNPs-modified GCE was investigated for the electrochemical determination of hydrogen peroxide (H2O2). Further enhancement of electrochemical performances was obtained using a nanocomposite made of AgNPs and reduced graphene oxide (rGO)-modified GCE. The AuNPs-SPCE sensor was instead tested in the electrochemical sensing of riboflavin (RF). To our knowledge, this is the first paper reporting Rumex roseus plant extract as a source for the synthesis of metal nanoparticles and their use for developing simple, sensitive and reliable electrochemical sensors for H2O2 and RF
Evaluation of antioxidant, antinflammatory and antityrosinase potential of extracts from different aerial parts of Rhanterium suaveolens from Tunisia
AbstractThe genus Rhanterium (Asteraceae) is a widely distributed medicinal plant throughout western North Africa and some Rhanterium species are used in folk medicine. The aim of research was to investigate methanolic extracts from different parts (flowers, leaves, and stems) of Tunisian Rhanterium suaveolens as potential sources of bioactive products useful for healthy purposes. In particular, were analyzed the phenolic composition of these extracts and their antioxidant, antiâinflammatory, and antiâtyrosinase properties.The phytochemical analyses were performed using standard colorimetric procedures, HPLCâDAD and HPLCâDADâESIâMS. Then, several inâ
vitro cellâfree assays have been used to estimate the antioxidant/free radical scavenging capability of the extracts. Moreover, inâ
vitro, and inâ
vivo antiâmelanogenesis activities of these extracts were tested, respectively, with the tyrosinase inhibition assay and the Zebrafish embryo model. Finally, the antiâinflammatory potential of these extracts in an inâ
vitro model of acute intestinal inflammation in differentiated Cacoâ2 cells was evaluated.The R. suaveolens extracts under study appeared particularly rich in flavonols and hydroxycinnamic acids and all extracts appeared endowed with good antioxidant/free radical scavenging properties, being the flower extracts slightly more active than the others. Moreover, R. suaveolens flowers extract was able to inhibit inâ
vitro tyrosinase activity and exhibited bleaching effects on the pigmentation of zebrafish embryos. Furthermore, all extracts showed good antiâinflammatory activity in intestinal epithelial cells as demonstrated by the inhibition of TNFâαâinduced gene expression of ILâ6 and ILâ8.R. suaveolens aerial parts may be considered as a potential source of whitening agents, as well as of agents for the treatment of disorders related to oxidative stress and inflammation