The omnivorous Tyrolean Iceman: colon contents (meat, cereals, pollen, moss and whipworm) and stable isotope analyses

The contents of the colon of the Tyrolean Iceman who lived Ga. 5300 years ago include muscle fibres, cereal remains, a diversity of pollen, and most notably that of the hop hornbeam (Ostrya carpinifolia) retaining cellular contents, as well as a moss leaf (Neckera complanata) and eggs of the parasitic whipworm (Trichuris trichiura). Based almost solely on stable isotope analyses and ignoring the work on the colon contents, two recently published papers on the Iceman's diet draw ill- founded conclusions about vegetarianism and even veganism. Neither the pollen nor the moss is likely to have been deliberately consumed as food by the Iceman. All the available evidence concerning the Iceman's broad-based diet is reviewed and the significance of the colon contents for matters other than assessment of food intake is outlined

The FIREBIRD Instrument for Relativistic Electrons: Enabling Technologies for a Fast High-Sensitivity, Low-Power Space Weather Radiation Payload

Miniaturized instrument payloads on small satellite and nanosatellite platforms that are deployed in low Earth orbit are demonstrating cost effective weather monitoring platforms with increased temporal and spatial resolution compared to larger weather satellites. The NASA Earth Decadal Survey [1] states that improving the revisit time of microwave radiometers would significantly improve weather forecasting. Radiometers such as the Advanced Technology Microwave Sounder (ATMS) on Suomi National Polar-orbiting Partnership (Suomi-NPP) and the Joint Polar Satellite System-1 (JPSS-1), now NOAA-20, provide an average revisit rate of 7.6 hours; however, a constellation of six CubeSats in three orbital Low Earth Orbit (LEO) planes with microwave radiometers such as the Time-Resolved Observations of Precipitations structure and storm Intensity with a Constellation of Smallsats (TROPICS) mission would provide a refresh rate of better than 60 minutes. In order to effectively use CubeSats in a constellation as a weather monitoring platform, calibration must be used to provide measurements consistent with state of the art measurements, such as ATMS that has a NeDT at 300K of 0.5-3.0K [2]. In this work, we use the Joint Center for Satellite Data Assimilation (JCSDA) Community Radiative Transfer Model (CRTM) to simulate brightness temperatures (https://www.jcsda.noaa.gov/projects_crtm.php), which are used to assess miniaturized microwave radiometer radiometric biases. CRTM is a fast radiative transfer model that uses Fortran functions, structure variables, and coefficient data of the modeled sensor to simulate radiances. The user inputs surface characteristics, scan angles, and atmospheric profiles from sources such as radiosondes, Numerical Weather Prediction (NWP) models, and Global Positioning System Radio Occultation (GPSRO) measurements. The output of CRTM is a simulated brightness temperature that is used to correct radiometric biases in order to meet required instrument NeDT performance. We use radiosonde, GPSRO, and NWP ERA-5 atmospheric profiles in CRTM and compare the results to ATMS brightness temperatures and find an average difference in brightness temperature of 1.95 K, which is comparable to ATMS Integrated Calibration/Validation System (https://www.star.nesdis.noaa.gov/icvs/status_NPP_ATMS.php) reports which show channel bias variations of up to 2 K. We take a similar approach to provide calibration for the Micro-sized Microwave Atmospheric Satellite-2A (MicroMAS-2A), a 3U CubeSat that was launched on January 11th, 2018. MicroMAS-2A carries a 1U 10-channel passive microwave radiometer that provides imagery near 90 and 206 GHz, temperature sounding near 118 GHz, and moisture sounding near 183 GHz. We develop an approach for comparing MicroMas-2A brightness temperatures to radiosonde, GPSRO, and NWP ERA5 atmospheric profiles. Due to the scarcity of GPSRO and radiosonde profiles near the MicroMAS-2A data segments, we determine that NWP models will be the best option for radiance validation. After the next stage of calibration of MicroMAS-2A is completed, we will compare CRTM simulated radiances from ERA profiles to the initial sensor data, with expected results of channel bias variations of \u3c 2 K

Evidence for weathering and volcanism during the PETM from Arctic Ocean and Peri-Tethys osmium isotope records

Sudden global warming during the Paleocene–Eocene Thermal Maximum (PETM, 55.9 Ma) occurred because of the rapid release of several thousand gigatonnes of isotopically light carbon into the oceans and atmosphere; however, the cause of this release is not well understood. Some studies have linked carbon injection to volcanic activity associated with the North Atlantic Igneous Province (NAIP), while others have emphasised carbon cycle feedbacks associated with orbital forcing. This study presents the osmium isotope compositions of mudrocks that were deposited during the PETM at four locations (one from the Arctic Ocean, and three from the Peri-Tethys). The Os-isotope records all exhibit a shift of similar magnitude towards relatively radiogenic values across the PETM. This observation confirms that there was a transient, global increase in the flux of radiogenic Os from the weathering of continental rocks in response to elevated temperatures at that time. The tectonic effects of NAIP volcanic emplacement near the onset of the PETM is recorded by anomalously radiogenic Os-isotope compositions of PETM-age Arctic Ocean samples, which indicate an interval of hydrographic restriction that can be linked tectonic uplift due to hotspot volcanism in the North Atlantic seaway. The Peri-Tethys data also document a transient, higher flux of unradiogenic osmium into the ocean near the beginning of the PETM, most likely from the weathering of young mafic rocks associated with the NAIP. These observations support the hypothesis that volcanism played a major role in triggering the cascade of environmental changes during the PETM, and highlight the influence of paleogeography on the Os isotope characteristics of marine water masses

Structural and Functional Deficits in a Neuronal Calcium Sensor-1 Mutant Identified in a Case of Autistic Spectrum Disorder

Neuronal calcium sensor-1 (NCS-1) is a Ca2+ sensor protein that has been implicated in the regulation of various aspects of neuronal development and neurotransmission. It exerts its effects through interactions with a range of target proteins one of which is interleukin receptor accessory protein like-1 (IL1RAPL1) protein. Mutations in IL1RAPL1 have recently been associated with autism spectrum disorders and a missense mutation (R102Q) on NCS-1 has been found in one individual with autism. We have examined the effect of this mutation on the structure and function of NCS-1. From use of NMR spectroscopy, it appeared that the R102Q affected the structure of the protein particularly with an increase in the extent of conformational exchange in the C-terminus of the protein. Despite this change NCS-1(R102Q) did not show changes in its affinity for Ca2+ or binding to IL1RAPL1 and its intracellular localisation was unaffected. Assessment of NCS-1 dynamics indicated that it could rapidly cycle between cytosolic and membrane pools and that the cycling onto the plasma membrane was specifically changed in NCS-1(R102Q) with the loss of a Ca2+ -dependent component. From these data we speculate that impairment of the normal cycling of NCS-1 by the R102Q mutation could have subtle effects on neuronal signalling and physiology in the developing and adult brain

Microsatellite analysis to estimate genetic relationships among five bulgarian sheep breeds

Herein, genetic relationships among five breeds of Bulgarian sheep were estimated using microsatellite markers. The total number of alleles identified was 226 at the 16 loci examined. DA distance values were used for phylogenetic tree construction with the UPGMA algorithm. The two Tsigai and two Maritza populations were found to be geneticallvery closely related to each other y (0.198, and 0.258 respectively). The Pleven Black Head population was distinct from the other four. These results could be useful for preserving genes in these breeds, thereby ensuring their preservation in Bulgaria

Internal mammary lymph node recurrence: rare but characteristic metastasis site in breast cancer

<p>Abstract</p> <p>Background</p> <p>To assess the frequency of IMLN recurrence, its associated risk factors with disease-free interval (DFI) and its predicting factors on overall survival time.</p> <p>Methods</p> <p>133 cases of breast cancer IMLN recurrence were identified via the computerized CT reporting system between February 2003 and June 2008, during which chest CT for patients with breast cancer (n = 8867) were performed consecutively at Cancer Hospital, Fudan University, Shanghai, China. Patients' charts were retrieved and patients' characteristics, disease characteristics, and treatments after recurrence were collected for analysis. The frequency was 1.5% (133/8867).</p> <p>Results</p> <p>IMLN recurrence was presented as the first metastatic site in 121 (91%) patients while 88 (66.2%) had other concurrent metastases. Typical chest CT images included swelling of the IMLN at the ipsilateral side with local lump and sternal erosion located mostly between the second and third intercostal space. The median disease-free interval (DFI) of IMLN recurrence was 38 months. The independent factors that could delay the IMLN recurrence were small tumor size (HR 0.5 95%CI: 0.4 - 0.8; <it>p </it>= 0.002), and positive ER/PR disease (HR 0.6, 95% CI: 0.4 - 0.9; <it>p </it>= 0.006). The median survival time after IMLN recurrence was 42 months, with a 5-year survival rate of 30%. Univariate analysis showed four variables significantly influenced the survival time: DFI of IMLN recurrence (p = 0.001), no concurrent distant metastasis (p = 0.024), endocrine therapy for patients with positive ER/PR (p = 0.000), radiotherapy (p = 0.040). The independent factors that reduced the death risk were no concurrent distant metastases (HR: 0.7, 95% CI: 0.4 - 0.9; <it>p </it>= 0.031), endocrine therapy for patients with positive ER/PR status (HR: 0.2, 95% CI: 0.1 - 0.5; <it>p </it>= 0.001) and palliative radiotherapy (HR: 0.3, 95% CI: 0.1- 0.9; <it>p </it>= 0.026).</p> <p>Conclusions</p> <p>The risk of IMLN recurrence is low and there are certain characteristics features on CT images. ER/PR status is both a risk factor for DFI of IMLN recurrence and a prognostic factor for overall survival after IMLN recurrence. Patients with only IMLN recurrence and/or local lesion have a good prognosis.</p