Pengujian Aktivitas Antibakteri Ekstrak Etanol Daun Kersen (Muntingia Calabura L.) terhadap Bakteri Penyebab Jerawat

Daun kersen (Muntingia calabura L) merupakan tanaman yang banyak tumbuh di Indonesia,tidak mengenal musim dan digunakan sebagai obat karena memiliki banyak khasiat salah satunya sebagai obat jerawat. Komponen senyawa kimia flavonoid, tannin dan saponin yang terdapat pada daun kersen diduga sebagai antibakteri. Tujuan penelitian ini untuk mengetahui apakah ekstrak etanol daun kersen mempunyai aktivitas antibakteri terhadap Staphylococcus epidermidis. Uji aktivitas antibakteri diakukan dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kersen memiliki kemampuan menghambat pertumbuhan Staphylococcus epidermidis pada konsentrasi 1ppm, 3ppm, 5ppm, 9ppm, ekstrak etanol daun kersen efektif menghambat pertumbuhan Staphylococcus epidermidis

ISOLASI DAN IDENTIFIKASI KOMPONEN KIMIA EKSTRAK N-HEKSAN DAUN PALA (Myristica fragrans)

Nutmeg plant is a multi-purpose plant because each part can be utilized in the industry. Nutmeg  leaves contain essential oils and phenolic compounds. Essential oils are used as traditional medicine ingredients and as raw material for the manufacture of cosmetics, soaps, perfumes and others. This study aimed to isolate and identify the chemical components in the leaves of nutmeg (Myristica fragrans). Have done isolation and Identification of chemical components of the methanol extract of leaves pala (Myristica fragrans.) of 500 grams to determine the class of chemical components caontained in the leaf pala (Myristica fragrans.). The methanol extract which obtained by maseartion method 9,45 grams continued by solid liquid partition with n-hexane 4,61 grams extract. N-hexan extract isolated by column chromatography method obtained 37 bottles vials. Fraction D of the merger based on the appearance of spotting the chromatograms isolated by preparative thin-layer chromatography method, obtained 3 band. D.1 fractions isolated by TLC obtained a stain. In fractions spotting D.1 is obtained by testing a single two-dimentional TLC ang the elution system of multi eluen. Interpretation UV-Visible spectra data showed maximum  absorption at a wavelength 0f 273 nm and showed infrared fungtional group (OH) on the wave number (3927.78 cm-1, 3828.59 cm-1, 3743.30 cm-1, 3387.93 cm-1), group (CH) on the wave number (2925.93 cm-1, 2859.02 cm-1, 2364.17 cm-1), alken group (C = C) aromatik on the wave number (1642.54 cm-1, 1565.88 cm-1, 1516.65 cm-1, 1458.92 cm-1, 1114.33 cm-1), benzene group on the wave number ( 698.24 cm-1). Single spots obtained gave a positive reaction to spotting visualizer class of  flavonoids. Key words : Nutmeg , Isolation, Essential Oils

PENGUJIAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) TERHADAP BAKTERI PENYEBAB JERAWAT

Daun kersen (Muntingia calabura L) merupakan tanaman yang banyak tumbuh di Indonesia,tidak mengenal musim dan digunakan sebagai obat karena memiliki banyak khasiat salah satunya sebagai obat jerawat. Komponen senyawa kimia flavonoid, tannin dan saponin yang terdapat pada daun kersen diduga sebagai antibakteri. Tujuan penelitian ini untuk mengetahui apakah ekstrak etanol daun kersen mempunyai aktivitas antibakteri terhadap Staphylococcus epidermidis. Uji aktivitas antibakteri diakukan dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kersen memiliki kemampuan menghambat pertumbuhan Staphylococcus epidermidis pada konsentrasi 1ppm, 3ppm, 5ppm, 9ppm, ekstrak etanol daun kersen efektif menghambat pertumbuhan Staphylococcus epidermidis

Kajian Farmakognostik Herba Meniran Hijau (Phyllanthus Niruri L.) Dan Herba Meniran Merah (Phyllanthus Urinaria L.)

Herba meniran secara empiris telah lama digunakan oleh masyarakat sebagai obat tradisional. Ditinjau dari prospek yang sangat potensial sebagai bahan obat maka perlu dilakukan kajian farmakognostik sampel untuk pengendalian mutu dan keaslian simplisia. Penelitian ini bertujuan memberikan dasar ilmiah mengenai gambaran farmakognostik secara kualitatif dan kuantitatif. Hasil penelitian secara kualitatif dan kuantitatif telah dideskripsikan. Kajian morfologi pada daun herba meniran hijau (Phyllanthus niruri L.) dan daun herba meniran merah (Phyllanthus urinaria L.) memiliki bentuk yang sama. Pada batang memiliki perbedaan, meniran hijau percabangannya monopodial dan berwarna hijau sedang meniran merah percabangannya simpodial dan berwarna merah. Pada akar sama-sama berakar tunggang serta berwarna putih kekuningan. Berdasarkan kajian anatomi pada meniran hijau (Phyllanthus niruri L.) dan Meniran merah (Phyllanthus urinaria L.) memiliki bentuk yang sama baik dari daun, batang maupun akarnya. Berdasarkan kajian identifikasi kandungan kimianya pada meniran hijau (Phyllanthus niruri L.) mengandung tanin (katekol), saponin dan karbohidrat, sedangkan pada meniran merah (Phyllanthus urinaria L.) hanya mengandung tanin (katekol) dan saponin

Standarisasi Ekstrak Etanol Buah Bintaro (Cerbera odollam Gaertn.) Sebagai Obat Tradisional

This research was conducted with the aim of examining the utilization of bintaro fruit extract (Cerbera odollam Gaertn.) as a traditional medicine that meets quality standards, so that the extract standardization process was carried out by determining specific and non-specific parameters.Based on the enormous potential of the Bintaro plant as a natural ingredient that can be used as traditional medicine, it is necessary to standardize the Bintaro fruit extract to determine the quality and safety of the extract raw materials used to support health. The research was conducted by determining the standardization of data on the determination of specific and non-specific parameters. The specific parameters that have been carried out include the identity of the extract, organoleptic test, the content of water soluble compounds 19.7%, the content of ethanol soluble compounds 6.96%, the content of positive extract compounds containing alkaloids, flavonoids, terpenoids, tannins and saponins. Non-specific parameters that have been carried out include drying shrinkage of 0.197%, moisture content of 2.124%, total ash content of 3.65%, acid soluble ash content of 1.3%, metal contamination of Pb and Cd 0.02 µg/g, microbial contamination of ALT bacteria. 0 colonies and 20 colonies of yeast. The results of the study met the standardization of extract quality.The data that will be obtained is expected to be additional information about the ethanolic extract of bintaro fruit (Cerbera odollam Gaertn.) that meets quality standards as traditional medicine, so as to maintain consistency and uniformity of efficacy and keep active compounds consistently measurable between treatments, and can maintain safety on consumers

KAJIAN FARMAKOGNOSTIK HERBA MENIRAN HIJAU (Phyllanthus niruri L.) dan HERBA MENIRAN MERAH (Phyllanthus urinaria L.)

Herba meniran secara empiris telah lama digunakan oleh masyarakat sebagai obat tradisional. Ditinjau dari prospek yang sangat potensial sebagai bahan obat maka perlu dilakukan kajian farmakognostik sampel untuk pengendalian mutu dan keaslian simplisia. Penelitian ini bertujuan memberikan dasar ilmiah mengenai gambaran farmakognostik secara kualitatif dan kuantitatif. Hasil penelitian secara kualitatif dan kuantitatif telah dideskripsikan. Kajian morfologi  pada daun  herba meniran hijau (Phyllanthus niruri L.) dan daun  herba meniran merah (Phyllanthus urinaria L.) memiliki bentuk yang  sama. Pada batang memiliki perbedaan, meniran hijau percabangannya monopodial dan berwarna hijau sedang meniran merah percabangannya simpodial dan berwarna merah. Pada akar sama-sama berakar tunggang serta berwarna putih kekuningan. Berdasarkan kajian anatomi pada meniran hijau (Phyllanthus niruri L.) dan Meniran merah (Phyllanthus urinaria L.) memiliki bentuk yang sama baik dari daun, batang maupun akarnya. Berdasarkan kajian identifikasi kandungan kimianya pada meniran hijau (Phyllanthus niruri L.) mengandung tanin (katekol), saponin dan karbohidrat, sedangkan pada meniran merah (Phyllanthus urinaria L.) hanya mengandung tanin (katekol) dan saponin

Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

AbstractBiancaea sappan  (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometri

Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala (Etlingera elatior (Jack) R.M.Sm) Menggunakan Metode DPPH

Patikala (Etlingera elatior (Jack) R.M.Sm) is a plant of the Zingiberaceae family. Patikala (E. elatior (Jack) R.M.Sm) have bioactive compounds are flavonoids that act as antioxidants. The purpose of this study was to measure free radical activity in the methanol extract of the fruit and leaves Patikala (E. elatior (Jack) R.M.Sm). Extraction of flowers and leaf samples by maceration method using methanol. Qualitative analysis of chemical compounds with certain eluent using Thin Layer Chromatography (TLC) to determine the group of active compounds in the extract. Antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 517 nm and compared with the power of antioxidant quersetin. The results showed that the methanol extract rendemen is at 1.935% for flowers and leaves at 5.17%. Antioxidant activity of the methanol extract of the flowers patikala (E. elatior (Jack) R.M.Sm) have low antioxidant activity with the value IC50 101.84 mg / mL and methanol extracts of leaves Patikala (E. elatior (Jack) R.MSm) have strong antioxidant activity with value IC50 30,65 mg / mL. This potential is lower than that of quercetin IC50 value of 5.35 mg / m