Profil Hormon Testosteron Rusa Timor (Cervus Timorensis) Jantan Dalam Satu Siklus Ranggah (Testosterone Hormone Profile in One Antler Cycle of Timor Deer (Cervus Timorensis) Stag)

The growth and replacement of stags antler on temperate species is physiologically linked to the seasonal rhythms of reproductive hormones. The objective of this research was to determine the pattern of testosterone hormone secretion in timor stags during one annual antler cycle which could be used to determine the active reproductive period of the stag. This research was conducted for 18 months in four adult timor stags (4 – 6 years of age). Blood samples were collected at three weeks intervals for analysis of testosterone hormone using the radioimmunoassay (RIA) method during one antler cycle (i.e. velvet, hard antler and casting stage). Results of this research showed significant difference (

Deer Frozen Semen Quality in Tris Sucrose and Tris Glucose Extender with Different Glycerol Concentrations

In order to improve Timor deer (Cervus timorensis) frozen semen quality, the influence of sugar and glycerol concentration on semen characteristics of sperm was investigated. The semen was collected from five sexually mature Timor deer using an electroejaculator. The semen was evaluated and divided into six equal tubes and diluted with Tris sucrose glycerol 10% (TSG10); Tris sucrose glycerol 12% (TSG12); Tris sucrose glycerol 14% (TSG14); Tris glucose glycerol 10% (TGG10); Tris glucose glycerol 12% (TGG12); and Tris glucose glycerol 14% (TGG14). The diluted semen was packed in 0.3 ml minitub straw, equilibrated at 5 oC for 4 hours and frozen on liquid nitrogen vapor for 10 minutes. The total of forward motility, viability, acrosome integrity and membrane integrity were assessed in fresh, after equilibration and after thawing. Results demonstrated that the percentage of sperm motility in TSG10was higher (P (63.93±7.23%). The sperm in TSG10 and TSG14 extender were superior in acrosome as well as in membrane integrity. It was concluded that Tris Sucrose with 10% glycerol protected Timor deer sperm better than other combinations

PROFIL HORMON TESTOSTERON RUSA TIMOR (Cervus timorensis) JANTAN DALAM SATU SIKLUS RANGGAH (Testosterone Hormone Profile in One Antler Cycle of Timor Deer (Cervus timorensis) stag)

The growth and replacement of stags antler on temperate species is physiologically linked to the seasonal rhythms of reproductive hormones. The objective of this research was to determine the pattern of testosterone hormone secretion in timor stags during one annual antler cycle which could be used to determine the active reproductive period of the stag. This research was conducted for 18 months in four adult timor stags (4 – 6 years of age). Blood samples were collected at three weeks intervals for analysis of testosterone hormone using the radioimmunoassay (RIA) method during one antler cycle (i.e. velvet, hard antler and casting stage). Results of this research showed significant difference (

THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN

The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY) diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer using electroejaculator. Immediately after collection the semen was evaluated macroscopic and microscopically. After initial evaluation, the semen was divided into three tubes and extended with Tris egg yolk with three different glycerol concentrations, which were 10% (TGEY10); 12% (TGEY12) and 14% (TGEY14). The sperm motility, viability, acrosome intact and membrane intact were evaluated in raw semen, after equilibration and after thawing. The results showed that there were no differences (p>0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact in TGEY10 (52.50±5.89%) and TGEY14 (51.50±4.12 %) were higher (p<0.05) than in TGEY12 (49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deer semen cryopreservation

Deer Frozen Semen Quality in Tris Sucrose and Tris Glucose Extender with Different Glycerol Concentrations

In order to improve Timor deer (Cervus timorensis) frozen semen quality, the influence of sugar and glycerol concentration on semen characteristics of sperm was investigated.  The semen was collected from five sexually mature Timor deer using an electroejaculator. The semen was evaluated and divided into six equal tubes and diluted with Tris sucrose glycerol 10% (TSG10); Tris sucrose glycerol 12% (TSG12); Tris sucrose glycerol 14% (TSG14); Tris glucose glycerol 10% (TGG10); Tris glucose glycerol 12% (TGG12); and Tris glucose glycerol 14% (TGG14). The diluted semen was packed in 0.3 ml minitub straw, equilibrated at 5 oC for 4 hours and frozen on liquid nitrogen vapor for 10 minutes. The total of forward motility, viability, acrosome integrity and membrane integrity were assessed in fresh, after equilibration and after thawing. Results demonstrated that the percentage of sperm motility in TSG10was higher (P (63.93±7.23%). The sperm in TSG10 and TSG14  extender were superior in acrosome as well as in membrane integrity. It was concluded that Tris Sucrose with 10% glycerol protected Timor deer sperm better than other combinations

Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature

The successful sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots. Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63%) and TEYS (21.83 ± 15.92%) were higher compare to TEYF (4,00 ± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06) and TEYS (24.00 ± 22.59%) (P<0.05) were significantly higher compare to TEYF (4.00 ± 0.00%). It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used for artificial insemination after 12 hour storage

Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature

The successful  sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in  Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates  in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots.  Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63%) and TEYS (21.83 ± 15.92%) were higher compare to TEYF (4,00 ± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06) and TEYS (24.00 ± 22.59%) (P0.05) were significantly higher compare to TEYF (4.00 ± 0.00%).  It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used  for artificial insemination after 12 hour storage. Key Words: Liquid Semen, Deer, Room Temperature, Carbohydrat

THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN

The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY)diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer usingelectroejaculator. Immediately after collection the semen was evaluated macroscopic andmicroscopically. After initial evaluation, the semen was divided into three tubes and extended with Trisegg yolk with three different glycerol concentrations, which were 10% (TGEY10); 12% (TGEY12) and14% (TGEY14). The sperm motility, viability, acrosome intact and membrane intact were evaluated inraw semen, after equilibration and after thawing. The results showed that there were no differences(p&gt;0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact inTGEY10 (52.50±5.89%) and TGEY14 (51.50±4.12 %) were higher (p&lt;0.05) than in TGEY12(49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deersemen cryopreservation

Modified Chest X-Ray Scoring System in Evaluating Severity of COVID-19 Patient in Dr. Soetomo General Hospital&nbsp; Surabaya, Indonesia

Rosy Setiawati,1 Anita Widyoningroem,1 Triwulan Handarini,1 Fierly Hayati,1 Agnes Triana Basja,1 Atrikha Rahma Dyana Surya Putri,1 Merlin Guntur Jaya,2 Jessica Andriani,2 Melina Rosita Tanadi,2 Imran Harsam Kamal2 1Department of Radiology, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Hospital Surabaya, Surabaya, Indonesia; 2Faculty of Medicine Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Rosy Setiawati 60286 Tel +62 878 5330 0019Email [email protected]: The management of COVID-19 patients requires efficiency and accuracy in methods of detection, identification, monitoring, and treatment feasible in every hospital. Aside from clinical presentations and laboratory markers, chest x-ray imaging could also detect pneumonia caused by COVID-19. It is also a fast, simple, cheap, and safe modality used for the management of COVID-19 patients. Established scoring systems of COVID-19 chest x-ray imaging include Radiographic Assessment of Lung Edema (RALE) and Brixia classification. A modified scoring system has been adopted from BRIXIA and RALE scoring systems and has been made to adjust the scoring system needs at Dr. Soetomo General Hospital, Indonesia. This study aims to determine the value of scoring systems through chest x-ray imaging in evaluating the severity of COVID-19.Methods: Data were collected from May to June of 2020 who underwent chest x-ray evaluation. Each image is then scored using three types of classifications: modified score, RALE score, and Brixia score. The scores are then analyzed and compared with the clinical conditions and laboratory markers to determine their value in evaluating the severity of COVID-19 infection in patients.Results: A total of 115 patients were males (51.1%) and 110 were females (48.9%). All three scoring systems are significantly correlated with the clinical severity of the disease, with the strengths of correlation in order from the strongest to weakest as Brixia score (p&lt; 0.01, correlation coefficient 0.232), RALE score (p&lt; 0.01, correlation coefficient 0.209), and Dr. Soetomo General Hospital score (p&lt; 0.01, correlation coefficient 0.194). All three scoring systems correlate significantly with each other. Dr. Soetomo General Hospital score correlates more towards Brixia score (p&lt; 0.01, correlation coefficient 0.865) than RALE score (p&lt; 0.01, correlation coefficient 0.855). Brixia to RALE score correlates with a coefficient of 0.857 (p&lt; 0.01).Conclusion: The modified scoring system can help determine the severity of the disease progression in COVID-19 patients especially in areas with shortages of facilities and specialists.Keywords: Brixia score, chest radiographs, COVID-19, modified score, RALE scor