Direct visualization of Ras proteins in spatially distinct cell surface microdomains

Localization of signaling complexes to specific microdomains coordinates signal transduction at the plasma membrane. Using immunogold electron microscopy of plasma membrane sheets coupled with spatial point pattern analysis, we have visualized morphologically featureless microdomains, including lipid rafts, in situ and at high resolution. We find that an inner-plasma membrane lipid raft marker displays cholesterol-dependent clustering in microdomains with a mean diameter of 44 nm that occupy 35% of the cell surface. Cross-linking an outer-leaflet raft protein results in the redistribution of inner leaflet rafts, but they retain their modular structure. Analysis of Ras microlocalization shows that inactive H-ras is distributed between lipid rafts and a cholesterol-independent microdomain. Conversely, activated H-ras and K-ras reside predominantly in nonoverlapping, cholesterol-independent microdomains. Galectin-1 stabilizes the association of activated H-ras with these nonraft microdomains, whereas K-ras clustering is supported by farnesylation, but not geranylgeranylation. These results illustrate that the inner plasma membrane comprises a complex mosaic of discrete microdomains. Differential spatial localization within this framework can likely account for the distinct signal outputs from the highly homologous Ras proteins

Unraveling the Nature of Charge Excitations in La2_2CuO4_4 with Momentum-Resolved Cu KK-edge Resonant Inelastic X-ray Scattering

Results of model calculations using exact diagonalization reveal the orbital character of states associated with different Raman loss peaks in Cu $K$-edge resonant inelastic X-ray scattering (RIXS) from La$_{2}$CuO$_{4}$. The model includes electronic orbitals necessary to highlight non-local Zhang-Rice singlet, charge transfer and $d$-$d$ excitations, as well as states with apical oxygen 2$p_z$ character. The dispersion of these excitations is discussed with prospects for resonant final state wave-function mapping. A good agreement with experiments emphasizes the substantial multi-orbital character of RIXS profiles in the energy transfer range 1-6 eV.Comment: Original: 4.5 pages. Replaced: 4 pages and 4 figures with updated content and reference

Growing Plants in Water.

8 p

Dependence of e-cloud on the longitudinal bunch profile: studies in the PS & extension to the HL-LHC

Recent studies have shown that the prospects for significantly increasing bunch intensities in the LHC for the luminosity upgrade (HL-LHC) may be severely limited by the available cryogenic cooling capacity and the electron-cloud (EC) driven beam instability. However, it is planned that during the HL-LHC era the bunch intensities in the LHC will go up by nearly a factor of two compared to the LHC-design values. This motivates the exploration of additional EC mitigation techniques that can be adopted in addition to those already in place. Preliminary simulations indicated that long flat bunches can be beneficial over Gaussian bunches to reduce the EC build up. Rigorous studies using realistic bunch profiles have never been done. Therefore, we have undertaken an in-depth investigation in the CERN 26 GeV PS to see if we can validate the previous findings and, in particular, if flattening the bunch can mitigate the EC. Here we present the results from dedicated EC measurements in the PS using a variety of bunch shapes and a comparison with simulations. Finally, we investigate if reshaping the bunch profiles using a 2nd harmonic rf cavity can mitigate EC in the HL-LHC

Controlling pulse propagation in optical fibers through nonlinearity and dispersion management

In case of the nonlinear Schr\"odinger equation with designed group velocity dispersion, variable nonlinearity and gain/loss; we analytically demonstrate the phenomenon of chirp reversal crucial for pulse reproduction. Two different scenarios are exhibited, where the pulses experience identical dispersion profiles, but show entirely different propagation behavior. Exact expressions for dynamical quasi-solitons and soliton bound-states relevant for fiber communication are also exhibited.Comment: 4 pages, 5 eps figure

Cholesterol-sensitive Cdc42 activation regulates actin polymerization for endocytosis via the GEEC pathway

Glycosyl-phosphatidylinositol (GPI)-anchored proteins (GPI-APs) are present at the surface of living cells in cholesterol dependent nanoscale clusters. These clusters appear to act as sorting signals for the selective endocytosis of GPI-APs via a Cdc42-regulated, dynamin and clathrin-independent pinocytic pathway called the GPI-AP-enriched early endosomal compartments (GEECs) pathway. Here we show that endocytosis via the GEECs pathway is inhibited by mild depletion of cholesterol, perturbation of actin polymerization or overexpression of the Cdc42/Rac-interactive-binding (CRIB) motif of neural Wiskott-Aldrich syndrome protein (N-WASP). Consistent with the involvement of Cdc42-based actin nanomachinery, nascent endocytic vesicles containing cargo for the GEEC pathway co-localize with fluorescent protein-tagged isoforms of Cdc42, CRIB domain, N-WASP and actin; high-resolution electron microscopy on plasma membrane sheets reveals Cdc42-labelled regions rich in green fluorescent protein-GPI. Using total internal reflection fluorescence microscopy at the single-molecule scale, we find that mild cholesterol depletion alters the dynamics of actin polymerization at the cell surface by inhibiting Cdc42 activation and consequently its stabilization at the cell surface. These results suggest that endocytosis into GEECs occurs through a cholesterol-sensitive, Cdc42-based recruitment of the actin polymerization machinery

Who is to blame? The relationship between ingroup identification and relative deprivation is moderated by ingroup attributions

Contradictory evidence can be found in the literature about whether ingroup identification and perceived relative deprivation are positively or negatively related. Indeed, theoretical arguments can be made for both effects. It was proposed that the contradictory findings can be explained by considering a hitherto unstudied moderator: The extent to which deprivation is attributed to the ingroup. It was hypothesised that identification would only have a negative impact on deprivation, and that deprivation would only have a negative impact on identification, if ingroup attributions are high. To test this, attributions to the ingroup were experimentally manipulated among British student participants (N = 189) who were asked about their perceived deprivation vis-à-vis German students, yield ing support for the hypotheses

Social comparisons and organizational support : implications for commitment and retention

Organizational support theory (OST) suggests that employees develop a general perception of the extent to which the organization values their contributions and cares about their well-being (perceived organizational support – POS), and respond to that support through attitudes and behaviors that are beneficial toward the organization. Although OST emphasizes both social exchange and self-enhancement processes, most accounts of POS’s effects are rooted in social exchange. For example, POS’s linkages with commitment and retention have been explained as an exchange of support for positive attitudes and continued employment. This research sheds light on self-enhancement’s less-understood role in fostering these reactions by demonstrating the influence of social comparison effects. Drawing on a sample of 342 employees nested in 82 work-units of a US hospitality company, our analysis demonstrates that favorable POS comparisons with peers in one’s work-unit are positively associated with commitment and retention, whereas unfavorable comparisons are negatively related. Results also show that comparisons taking place in less-supported work-units have stronger impact than comparisons made in those with better support. Our findings extend OST by revealing the importance of social comparisons in engendering responses to organizational support, and in so doing potentially explicate the differential ways social exchange and self-enhancement operate with regard to POS

Application of Hansch’s Model to Capsaicinoids and Capsinoids: A Study Using the Quantitative Structure−Activity Relationship. A Novel Method for the Synthesis of Capsinoids

We describe a synthetic approach for two families of compounds, the capsaicinoids and capsinoids, as part of a study of the quantitative relationship between structure and activity

Dynamic concentration of motors in microtubule arrays

We present experimental and theoretical studies of the dynamics of molecular motors in microtubule arrays and asters. By solving a convection-diffusion equation we find that the density profile of motors in a two-dimensional aster is characterized by continuously varying exponents. Simulations are used to verify the assumptions of the continuum model. We observe the concentration profiles of kinesin moving in quasi two-dimensional artificial asters by fluorescent microscopy and compare with our theoretical results.Comment: 4pages, 4 figures revte