Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply.

Lipoprotein lipase (LPL) mediates hydrolysis of triglycerides (TGs) to supply free fatty acids (FFAs) to tissues. Here, we show that LPL activity is also required for hematopoietic stem progenitor cell (HSPC) maintenance. Knockout of Lpl or its obligatory cofactor Apoc2 results in significantly reduced HSPC expansion during definitive hematopoiesis in zebrafish. A human APOC2 mimetic peptide or the human very low-density lipoprotein, which carries APOC2, rescues the phenotype in apoc2 but not in lpl mutant zebrafish. Creating parabiotic apoc2 and lpl mutant zebrafish rescues the hematopoietic defect in both. Docosahexaenoic acid (DHA) is identified as an important factor in HSPC expansion. FFA-DHA, but not TG-DHA, rescues the HSPC defects in apoc2 and lpl mutant zebrafish. Reduced blood cell counts are also observed in Apoc2 mutant mice at the time of weaning. These results indicate that LPL-mediated release of the essential fatty acid DHA regulates HSPC expansion and definitive hematopoiesis

Clinical study of the effect of refractive status on stereopsis in children with intermittent exotropia

Abstract Background Many studies have shown that patients with intermittent exotropia have different degrees of damage to their stereopsis function. The purpose of this study is to explore the effect of different refractive status on stereopsis in children with intermittent exotropia(IXT). Methods We assessed 90 children of ages 4~ 16 years with intermittent exotropia at the Second Affiliated Hospital of Dalian Medical University during the years 2016–2017. According to their refractive status, the patients were divided into hyperopia group(spherical equivalent > or = − 1.00 diopters), myopia group(spherical equivalent  or = 1.50 diopter). The distant stereopsis of the patient was checked by the synoptophore, and the near stereopsis of the patients was checked by the Titmus stereogram. Then, we compared the difference between distant stereopsis and near stereopsis in the four groups. Results (1)The retention rates of distant stereopsis in the hyperopia group, emmetropia group, myopia group and anisometropia group were 33.3, 45.2, 34.6, and 33.3%, respectively. There was no significant difference between the groups with different refractive status. (2)The retention rates of near stereopsis in the hyperopia group, emmetropia group, myopia group and anisometropia group were 66.7, 83.9, 80.8, and 55.6%, respectively. There were statistically significant differences between the emmetropia group and the anisometropia group (P = 0.030). (3)The retention rate of distant stereopsis in children with intermittent exotropia was 37.8%, and the retention rate of near stereopsis was 74.4%. The difference between the two groups was statistically significant (P = 0.019). Conclusions The damage to distant stereopsis in children with intermittent exotropia is more serious than that to near stereopsis. The damage to near stereopsis in children with intermittent exotropia and anisometropiais more serious

Environmental factors driving the succession and differentiation of ecological strategy spectrum in tropical lowland rain forest

We aimed to determine the environmental driving factors underpinning successional changes in a tropical lowland rain forest in Hainan. Using hectare scale plots, we evaluated woody plant community composition and collected a variety of environmental variables. We found that the ecological strategy spectrum of communities in the four succession stages differentiated along the C-S axis, but no major communities were found in the R strategy group. The spectrum of ecological strategy of different communities is significantly different with succession, among which C strategy group is on the rise, S strategy group is on the decline, and the intermediate (INT) group strategy is on the rise after the first decline. For the C strategy group, population density and canopy openness explain most of the differences. The S strategy group are explained by slope and population density, while the first two explanatory factors of intermediate strategy group differences are slope and soil total potassium content. The change of plant ecological strategy is the result of comprehensive action of biotic factors, topographic factors and soil factors, among which biotic factors play a leading role

Identification of novel genes and networks governing hematopoietic stem cell development.

Hematopoietic stem cells (HSCs) are capable of giving rise to all blood cell lineages throughout adulthood, and the generation of engraftable HSCs from human pluripotent stem cells is a major goal for regenerative medicine. Here, we describe a functional genome-wide RNAi screen to identify genes required for the differentiation of embryonic stem cell (ESC) into hematopoietic stem/progenitor cells (HSPCs) in vitro We report the discovery of novel genes important for the endothelial-to-hematopoietic transition and subsequently for HSPC specification. High-throughput sequencing and bioinformatic analyses identified twelve groups of genes, including a set of 351 novel genes required for HSPC specification. As in vivo proof of concept, four of these genes, Ap2a1, Mettl22, Lrsam1, and Hal, are selected for validation, confirmed to be essential for HSPC development in zebrafish and for maintenance of human HSCs. Taken together, our results not only identify a number of novel regulatory genes and pathways essential for HSPC development but also serve as valuable resource for directed differentiation of therapy grade HSPCs using human pluripotent stem cells

UAV-Assisted Three-Dimensional Spectrum Mapping Driven by Spectrum Data and Channel Model

As the number of civil aerial vehicles increase explosively, spectrum scarcity and security become an increasingly challenge in both the airspace and terrestrial space. To address this difficulty, this paper presents an unmanned aerial vehicle-assisted (UAV-assisted) spectrum mapping system and a spectrum data reconstruction algorithm driven by spectrum data and channel model are proposed. The reconstruction algorithm, which includes a model-driven spectrum data inference method and a spectrum data completion method with uniformity decision mechanism, can reconstruct limited and incomplete spectrum data to a three-dimensional (3D) spectrum map. As a result, spectrum scarcity and security can be achieved. Spectrum mapping is a symmetry-based digital twin technology. By employing an uniformity decision mechanism, the proposed completion method can effectively interpolate spatial data even when the collected data are unevenly distributed. The effectiveness of the proposed mapping scheme is evaluated by comparing its results with the ray-tracing simulated data of the campus scenario. Simulation results show that the proposed reconstruction algorithm outperforms the classical inverse distance weighted (IDW) interpolation method and the tensor completion method by about 12.5% and 92.3%, respectively, in terms of reconstruction accuracy when the collected spectrum data are regularly missing, unevenly distributed and limited

Optimal site selection for the remote-monitoring sulfur content of ship fuels in ports

The remote monitoring method based on air-quality monitoring sensors is a common way to monitor the FSC (fuel sulfur content) of oils for ships. Considering the influences of geographical environments, atmospheric conditions, regional ship traffic flow, emission characteristics of ships, and height of monitoring sensors on the monitor station chosen, a new method was proposed to optimize the site selection for monitoring the FSC of fuel oils used by ships in waters of the port. SO2 numeric simulation and observation from sensors were integrated to estimate the FSCs. The proposed method was utilized to recommend the sites of the fixed sniffing monitoring stations in Yantian port, Shenzhen, China from June and July 2018. The results showed that the experimental stations could monitor FSCs, and the relative difference between the estimated and actual FSCs of ships was 16.34%. The proposed method for recommending sites of FSC monitoring sensors contributed to intelligently supervising air pollutants emitted from ships and fuel oils of ships in the emission control areas of China