Panel Discussion On The Management Of Allergies In Geriatric Patients†

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111236/1/jgs00790.pd

Gene expression profile of peripheral blood lymphocytes from renal cell carcinoma patients treated with IL-2, Interferon-α and dendritic cell vaccine

© The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 7 (2012): e50221, doi:10.1371/journal.pone.0050221.Lymphocytes are a key component of the immune system and their differentiation and function are directly influenced by cancer. We examined peripheral blood lymphocyte (PBL) gene expression as a biomarker of illness and treatment effect using the Affymetrix Human Gene ST1 platform in patients with metastatic renal cell carcinoma (mRCC) who received combined treatment with IL-2, interferon-?-2a and dendritic cell vaccine. We examined gene expression, cytokine levels in patient serum and lymphocyte subsets as determined by flow cytometry (FCM). Pre-treatment PBLs from patients with mRCC exhibit a gene expression profile and serum cytokine profile consistent with inflammation and proliferation not found in healthy donors (HD). PBL gene expression from patients with mRCC showed increased mRNA of genes involved with T-cell and TREG-cell activation pathways, which was also reflected in lymphocyte subset distribution. Overall, PBL gene expression post-treatment (POST) was not significantly different than pre-treatment (PRE). Nevertheless, treatment related changes in gene expression (post-treatment minus pre-treatment) revealed an increased expression of T-cell and B-cell receptor signaling pathways in responding (R) patients compared to non-responding (NR) patients. In addition, we observed down-regulation of TREG-cell pathways post-treatment in R vs. NR patients. While exploratory in nature, this study supports the hypothesis that enhanced inflammatory cytotoxic pathways coupled with blunting of the regulatory pathways is necessary for effective anti-cancer activity associated with immune therapy. This type of analysis can potentially identify additional immune therapeutic targets in patients with mRCC.This work was supported by grants from the National Institutes of Health (RO1 CA5648, R21CA112761, P20RR016437, and P30CA023108)

Pulmonary arterial medial smooth muscle thickness in sudden infant death syndrome: an analysis of subsets of 73 cases

Previous studies addressing pulmonary artery morphology have compared cases of sudden infant death syndrome (SIDS) to controls but none have compared demographic profiles, exposure to potentially hypoxic risk factors and other pathologic variables in SIDS cases grouped according to pulmonary artery medial smooth muscle thickness. Aims: To compare the relative medial thickness (RMT) in alveolar wall arteries (AW) in SIDS cases with that in age-matched controls and 2. Compare demographic, clinical, and pathologic characteristics among three subsets of SIDS cases based upon alveolar wall (AW) RMT. Retrospective morphometric planimetry of all muscularized arteries in standardized right apical lung sections in 73 SIDS cases divided into three groups based on increasing AW RMT as well as 19 controls age-matched to 19 of the SIDS cases. SIDS and age-matched control cases did not differ with respect to AW RMT or other demographic variables. The SIDS group with the thickest AW RMT had significantly more males and premature birth than the other groups, but the groups did not differ for known clinical risk factors that would potentially expose them to hypoxia. Pathologic variables, including pulmonary inflammation, gastric aspiration, intra-alveolar siderophages, cardiac valve circumferences, and heart and liver weights, were not different between groups. Age was not significantly correlated with RMT of alveolar wall and pre-acinar arteries but was significant at p = .018 for small intra-acinar arteries. The groups were different for RMT of small pre-acinar and intra-acinar arteries, which increased with increasing AW RMT. Statistical differences should not necessarily be equated with clinical importance, however future research incorporating more quantified historical data is recommended

Ecology under lake ice

Winter conditions are rapidly changing in temperate ecosystems, particularly for those that experience periods of snow and ice cover. Relatively little is known of winter ecology in these systems, due to a historical research focus on summer "growing seasons." We executed the first global quantitative synthesis on under-ice lake ecology, including 36 abiotic and biotic variables from 42 research groups and 101 lakes, examining seasonal differences and connections as well as how seasonal differences vary with geophysical factors. Plankton were more abundant under ice than expected; mean winter values were 42.8% of summer values for chlorophyll a, 15.8% of summer phytoplankton biovolume, and 34.4% of summer zooplankton density. Dissolved nitrogen concentrations were typically higher during winter, and these differences were exaggerated in smaller lakes. Lake size also influenced winter-summer patterns for dissolved organic carbon (DOC), with higher winter DOC in smaller lakes. At coarse levels of taxonomic aggregation, phytoplankton and zooplankton community composition showed few systematic differences between seasons, although literature suggests that seasonal differences are frequently lake-specific, species-specific, or occur at the level of functional group. Within the subset of lakes that had longer time-series, winter influenced the subsequent summer for some nutrient variables and zooplankton biomass

Beds comprising debrite sandwiched within co-genetic turbidite: origin and widespread occurrence in distal depositional environments

Co-genetic debrite–turbidite beds occur in a variety of modern and ancient turbidite systems. Their basic character is distinctive. An ungraded muddy sandstone interval is encased within mud-poor graded sandstone, siltstone and mudstone. The muddy sandstone interval preserves evidence of en masse deposition and is thus termed a debrite. The mud-poor sandstone, siltstone and mudstone show features indicating progressive layer-by-layer deposition and are thus called a turbidite. Palaeocurrent indicators, ubiquitous stratigraphic association and the position of hemipelagic intervals demonstrate that debrite and enclosing turbidite originate in the same event. Detailed field observations are presented for co-genetic debrite–turbidite beds in three widespread sequences of variable age: the Miocene Marnoso Arenacea Formation in the Italian Apennines; the Silurian Aberystwyth Grits in Wales; and Quaternary deposits of the Agadir Basin, offshore Morocco. Deposition of these sequences occurred in similar unchannellized basin-plain settings. Co-genetic debrite–turbidite beds were deposited from longitudinally segregated flow events, comprising both debris flow and forerunning turbidity current. It is most likely that the debris flow was generated by relatively shallow (few tens of centimetres) erosion of mud-rich sea-floor sediment. Changes in the settling behaviour of sand grains from a muddy fluid as flows decelerated may also have contributed to debrite deposition. The association with distal settings results from the ubiquitous presence of muddy deposits in such locations, which may be eroded and disaggregated to form a cohesive debris flow. Debrite intervals may be extensive (> 26 x 10 km in the Marnoso Arenacea Formation) and are not restricted to basin margins. Such long debris flow run-out on low-gradient sea floor (< 0.1?) may simply be due to low yield strength (<<50 Pa) of the debris–water mixture. This study emphasizes that multiple flow types, and transformations between flow types, can occur within the distal parts of submarine flow events