Precision placement of fertiliser for optimising the early nutrition of vegetable crops : a review of the implications for the yield and quality of crops, and their nutrient use efficiency

The research outlined in this paper highlights the importance of the early nutrition of vegetable crops, and its long-term effects on their subsequent growth and development. Results are also presented to demonstrate how the nutrient supply during the establishment stages of young seedlings and transplants can be enhanced by targeting fertiliser to a zone close to their developing roots. Three different precision fertiliser placement techniques are compared for this purpose: starter, band or side-injected fertiliser. The use of each of these methods consistently produced the same (or greater) yields at lower application rates than those from conventional broadcast applications, increasing the apparent recovery of N, P and K, and the overall efficiency of nutrient use, while reducing the levels of residual nutrients in the soil. Starter fertilisers also advanced the maturity of some crops, and enhanced produce quality by increasing the proportions of the larger and/or more desirable marketable grades. The benefits of the different placement techniques are illustrated with selected examples from research at Warwick HRI using different vegetable crops, including lettuce, onion and carrot

Optimising the analysis of transcript data using high density oligonucleotide arrays and genomic DNA-based probe selection

Background: Affymetrix GeneChip arrays are widely used for transcriptomic studies in a diverse range of species. Each gene is represented on a GeneChip array by a probe-set, consisting of up to 16 probe-pairs. Signal intensities across probe-pairs within a probe-set vary in part due to different physical hybridisation characteristics of individual probes with their target labelled transcripts. We have previously developed a technique to study the transcriptomes of heterologous species based on hybridising genomic DNA (gDNA) to a GeneChip array designed for a different species, and subsequently using only those probes with good homology. Results: Here we have investigated the effects of hybridising homologous species gDNA to study the transcriptomes of species for which the arrays have been designed. Genomic DNA from Arabidopsis thaliana and rice (Oryza sativa) were hybridised to the Affymetrix Arabidopsis ATH1 and Rice Genome GeneChip arrays respectively. Probe selection based on gDNA hybridisation intensity increased the number of genes identified as significantly differentially expressed in two published studies of Arabidopsis development, and optimised the analysis of technical replicates obtained from pooled samples of RNA from rice. Conclusion: This mixed physical and bioinformatics approach can be used to optimise estimates of gene expression when using GeneChip arrays

Resting toucher: a time and motion analysis of elite lawn bowls

Whilst numerous investigations have explored the physical demands placed upon competitive sportspeople from a wide array of sports little is known about the physical demands placed on lawn bowlers. The purpose of this study was to ascertain the movement activities of Australian representative singles and pairs players and to determine the frequency and duration of these activities. One match each of two male and two female players (one singles and one pairs player per gender) were videotaped during an international tournament. During playback of the videotaped matches (n = 4), a single observer coded the players’ activities into five distinct categories (waiting, walking forward, walking backward, jogging and bowling) using a computerised video editing system (Gamebreaker™ Digital Video Analysis System). Field calibration of players over 30m for forward motions and 15m for the backward motion was performed to allow for the estimation of total distance covered during the match. Heart rate was monitored during each match. The duration of a match was found to be (mean ± SD) 1hr 28 ± 15mins. The total distance covered during each match was 2093 ± 276m. The mean percentage of match time spent in each motion was: waiting, 61.8 ± 9.3%; walking forward, 22.3 ± 5.6%; walking backward, 2.0 ± 0.4%; jogging, 1.1 ± 0.5%; and bowling, 8.5 ± 4.2%. Average heart rate was found to be 57 ± 7% of age-predicted HRmax with a maximum of 78 ± 9% of age-predicted HRmax. The results of this study suggest that playing lawn bowls at an international level requires light-moderate intensity activity similar to that reported for golf

The effects of introgression of the Submergence1 allele into rice cultivar IR64 on post-harvest seed dormancy and longevity

Introgressing the Submergence1 (Sub1) allele into chromosome 9 of high-yielding rice (Oryza sativa L.) cultivars introduced the flood-tolerant trait, but this chromosome also includes quantitative trait loci for seed storage survival. Loss in seed dormancy and viability during post-harvest storage were investigated in cvs IR64 and IR64-Sub1 to test if the introgression of Sub1 affected seed longevity. They were grown in the same controlled environment, mature seeds harvested, and ability to germinate monitored during subsequent hermetic storage at 40°C with 13.5-13.9% moisture content. The overlapping patterns of loss in dormancy and loss in viability during storage were quantified well by a multiplicative model, with similar responses in the two near-isogenic cultivars: both showed almost full dormancy initially with complete, similar, loss in dormancy during the first seven days of storage, loss in viability after 43 days, and 50% viability periods (p50) of 26.4-28.1 days. Hence, introgression of the submergence-tolerant allele Sub1A-1 in rice cv. IR64 did not affect seed dormancy or survival after harvest substantially, but duration to flowering was nine days longer and seed yield also greater in cv. IR64-Sub1

Foliar application of molybdenum reduces yield loss and pre-harvest sprouting in japonica rice seed subjected to simulated flooding during seed development and maturation

Flooding damages rice crops and its incidence is increasing. Foliar spray applications of molybdenum (100, 600 or 3000 mg Mo L-1), abscisic acid (ABA, 50 μM), or deionised water (control) were made to pot-grown plants of the Japonica rice cv. Gleva at flag leaf appearance to determine their effects on seed yield and pre-harvest sprouting after flooding. Plants were submerged , to simulate flooding, for four days from 20 or 30 days after anthesis (DAA). Seed yield per plant, seed weight, and pre-harvest sprouting were determined after harvest at 37 DAA. Seed yield and weight were substantially reduced and pre-harvest sprouting promoted by submergence, with greater sprouting after the later treatment. Prior treatment with molybdenum or ABA reduced pre-harvest sprouting (P P > 0.05), compared with deionised water. The molybdenum concentration in both sprouted and non-sprouted seeds harvested at 37 DAA responded positively to molybdenum application rate. Molybdenum application rate had no consistent effect on seed ABA concentration. Foliar application of 100 mg Mo L-1 at 200 L ha-1 provided the most promising mitigation of flood damage, but benefits were minor in the context of seed damage from submergence

Onion gene expression in response to ethylene and 1-MCP

Onion is regarded as a non-climacteric vegetable. In onions, however, ethylene can suppress sprouting while the ethylene binding inhibitor, 1-MCP (1- methylcyclopropene) can also suppress sprout growth yet, it is unknown how ethylene and 1-MCP elicit the same response. In this study, onions were treated with 10 μL L-1 ethylene or 1 μL L-1 1-MCP individually or in combination for 24 h at 20°C before or after curing (six weeks) at 20 or 28°C then stored at 1°C. Following curing, a subset of these same onions was stored separately under continuous air or ethylene (10 μL L- 1) at 1°C Onions treated with ethylene and 1-MCP in combination after curing for 24 h had reduced sprout growth as compared with the control 25 weeks after harvest. Sprout growth following storage beyond 25 weeks was only reduced through continuous ethylene treatment. This observation was supported by a higher proportion of down-regulated genes characterised as being involved in photosynthesis measured using a newly developed onion microarray. Physiological and biochemical data suggested that ethylene was being perceived in the presence of 1-MCP since sprout growth was reduced in onions treated with 1-MCP and ethylene applied in combination but not when applied individually. A cluster of probes representing transcripts up-regulated by 1-MCP alone but down-regulated by ethylene alone or in the presence of 1-MCP support this suggestion. Ethylene and 1-MCP both down52 regulated a probe tentatively annotated as an ethylene receptor as well as EIN3, suggesting that both treatments down-regulate the perception and signalling events of ethylene

Dipolar Bose-Einstein condensates with dipole-dependent scattering length

We consider a Bose-Einstein condensate of polar molecules in a harmonic trap, where the effective dipole may be tuned by an external field. We demonstrate that taking into account the dependence of the scattering length on the dipole moment is essential to reproducing the correct energies and for predicting the stability of the condensate. We do this by comparing Gross-Pitaevskii calculations with diffusion Monte Carlo calculations. We find very good agreement between the results obtained by these two approaches once the dipole dependence of the scattering length is taken into account. We also examine the behavior of the condensate in non-isotropic traps

Effect of soaking and misting on respiration rate, body surface temperature, and body temperature of heat stressed dairy cattle

Reducing heat stress is a key issue for dairy producers. Use of feedline soaking and supplemental airflow effectively reduces heat stress and increases milk production and profitability. High-pressure misting allows water to evaporate in the air, reduces air temperature, and increases relative humidity. Misting also soaks the skin of cattle, resulting in additional cooling as water evaporates from skin surfaces, similar to the cooling effect of feedline soaking. Impact of soaking frequency (5-, 10-, or 15-minute intervals) was compared to continuous high-pressure misting. Cows cooled with either system had lower respiration rates, body surface temperatures, and internal body temperatures than controls. Soaking cattle every 5 minutes or 5-minute soaking plus high-pressure misting produced similar body temperatures, but lower (P\u3c0.01) than those when soaking occurred every 10 or 15 minutes. Skin surface temperatures from the thurl, shoulder, and rear udder were less when cattle were cooled with high-pressure misting. Cattle cooled with high-pressure misting became soaked, thus the cooling effect is the combination of cooler air and water evaporation from the skin. These results indicate that either frequent soaking (every 5 minutes) or continuous high-pressure misting that soaks the skin could be equally effective in reducing heat stress in dairy cattle.; Dairy Day, 2003, Kansas State University, Manhattan, KS, 2003

Using genomic DNA-based probe-selection to improve the sensitivity of high-density oligonucleotide arrays when applied to heterologous species

High-density oligonucleotide (oligo) arrays are a powerful tool for transcript profiling. Arrays based on GeneChip® technology are amongst the most widely used, although GeneChip® arrays are currently available for only a small number of plant and animal species. Thus, we have developed a method to improve the sensitivity of high-density oligonucleotide arrays when applied to heterologous species and tested the method by analysing the transcriptome of Brassica oleracea L., a species for which no GeneChip® array is available, using a GeneChip® array designed for Arabidopsis thaliana (L.) Heynh. Genomic DNA from B. oleracea was labelled and hybridised to the ATH1-121501 GeneChip® array. Arabidopsis thaliana probe-pairs that hybridised to the B. oleracea genomic DNA on the basis of the perfect-match (PM) probe signal were then selected for subsequent B. oleracea transcriptome analysis using a .cel file parser script to generate probe mask files. The transcriptional response of B. oleracea to a mineral nutrient (phosphorus; P) stress was quantified using probe mask files generated for a wide range of gDNA hybridisation intensity thresholds. An example probe mask file generated with a gDNA hybridisation intensity threshold of 400 removed > 68 % of the available PM probes from the analysis but retained >96 % of available A. thaliana probe-sets. Ninety-nine of these genes were then identified as significantly regulated under P stress in B. oleracea, including the homologues of P stress responsive genes in A. thaliana. Increasing the gDNA hybridisation intensity thresholds up to 500 for probe-selection increased the sensitivity of the GeneChip® array to detect regulation of gene expression in B. oleracea under P stress by up to 13-fold. Our open-source software to create probe mask files is freely available http://affymetrix.arabidopsis.info/xspecies/ webcite and may be used to facilitate transcriptomic analyses of a wide range of plant and animal species in the absence of custom arrays