Work Life balance during Covid-19 Lockdown: Experiences of Women Academicians of Kashmir

Work-life balance has always been a challenge for all the working class but during the COVID-19 pandemic, it has added to the already existing challenges because the work life too has shifted to home through online mode. COVID-19 and the consequent lockdown throughout the world have diminished the boundary between the workplace and the home place. The work life and family life are to be lived and managed under the same space at the home. In a patriarchal society like India, within the working class the work-life balance of the working women has been a constant challenge. This is because of the gendered roles at the home which are principally considered to be the responsibility of the women of the family. Whether it is the house maintenance, childcare or the elderly care, these responsibilities have been associated with the women of the house only. Since after the lockdown in India, the work was shifted to online mode working women have faced numerous problems while delivering the dual duties of office and home simultaneously at the same time and same place. The paper aims to gain in-depth understanding of the experiences of women academicians regarding their work-life balance during COVID-19 pandemic. We have used the qualitative research approach to explore and analyze the challenges faced by the women working in higher education department to balance work and life during COVID-19 pandemic. Female academicians having at least one kid below the age of 15 years were taken into the study. The study used interview method for data collection. Both face to face and telephonic interviews were conducted with the participants keeping in view the social distancing rules and other lockdown measures. They study used inductive thematic analysis for the generation of the results. Three main themes emerged from the study. The themes along with the respective sub themes are : 1. Work related issues ( need to learn the online teaching tools for digital classes, erratic work schedule, stretched working hours, screen fatigue, camera consciousness, interrupted classes due to internet and connectivity problems) 2. Family Issues (elderly care issues, role conflict and guilt, children’s educational issues, maintenance of social relations, house help problems) 3. Personal Issues (burnout, lack of downtime, feelings of isolation)

HIV associated lupus like nephropathy

BACKGROUND: Human immunodeficiency virus type 1 (HIV-1)– seropositive patients are at a high risk for the development of a variety of acute and chronic renal diseases. Most patients with HIVAN are of African descent, presenting late in the course of their HIV-1 infection. The only reliable test to establish or rule out the presence of HIVAN (HIV associated nephropathy) is renal biopsy. The most common lesion associated with HIV is a focal segmental glomeruloscelerosis, but several times, other biopsyfindings may also be seen. Our patient had lupus nephritis like pathology picture. The therapeutic agents with the most promise are angiotensin-converting enzyme inhibitors and antiretroviral medications. Role of steroids are less well-defined although they have been used with success many times.CASE DETAILS: Our patient was a young male who presented with a  pulmonary renal syndrome like picture and wasting. On evaluation, he was found to be HIV-1 positive, and renal biopsy showed lupus nephritis like pathological picture. The patient was treated with HAART (Highly active anti retroviral therapy) , steroids and ACE inhibitors and showed an excellent response.CONCLUSION: The case highlights the fact that immune mediated  glomerulonephritis, although rare, can be the presenting feature of HIV infection and can be controlled, if not cured, with proper treatment.KEYWORDS: HIV, Glomerulonephritis, HIV associated nephropath

Feeding habits of pick handle barracuda (Sphyraena jello) Cuvier, 1829 along the coast of Pakistan

A one year combined study of both sexes was conducted from March 2017 to February 2018 to investigate the feeding habit of Pick handle Barracuda (Sphyraena jello) Cuvier, 1829 locally called as Safaid kund along the coast of Pakistan, a large species found in Pakistani water caught mainly by bottom set gillnet throughout the year. Samples studied at Karachi fish harbor, in commercial catch which is the major landing center of Pakistan. During this counting method was applied with naked eye. Mostly fishes characterized by 91.66%, cephalopod 5.66% and shrimp 2.78% made the highest and lowest stomach content respectively shoes carnivorous behavior of the species

Nanomedicines: Nano based Drug Delivery Systems Challenges and Opportunities

Nanomedicine and nano delivery systems, although relatively recent but fast-developing technology is one where nanoscale materials are used to function as diagnostic tools or to deliver therapeutic agents to specifically targeted sites in a controlled manner. It also provides many advantages in the management of human diseases. Recently, there has been a range of excellent uses of nanomedicine as chemotherapeutic agents, biological agents, immunotherapeutic agents, etc., for treatment of different diseases. In this chapter we discuss the recent developments and insights obtained in the field of nanomedicine. It provides a review of the numerous nano-based drug delivery systems that enhance the efficacy of new and old drugs. The new opportunities and challenges arising in the area of nanomedicine from therapeutic viewpoint are also addressed

Autophagy:A Versatile Player in the Progression of Colorectal Cancer and Drug Resistance

Colorectal cancer (CRC) is among the topmost malignancies for both genders. Despite the high incidence rate and advances in diagnostic tools, treatment in many cases is still ineffective. Most cancerous lesions in CRC begin as benign, followed by the development of invasive forms and metastases. The development of CRC has been linked to defects in autophagy, which plays both a pro-and anti-tumor role and is mainly context-dependent. Autophagy suppression could enhance apoptosis via p53 activation, or autophagy also promotes tumor progression by maintaining tumor growth and increasing resistance to chemotherapy. Autophagy promotes the invasion and metastasis of CRC cells via increased epithelial-mesenchymal transition (EMT). Moreover, dysbiosis of gut microbiota upregulated autophagy and metastasis markers. Autophagy responses may also modulate the tumor microenvironment (TME) via regulating the differentiation process of several innate immune cells. Treatments that promote tumor cell death by stimulating or inhibiting autophagy could be beneficial if used as an adjunct treatment, but the precise role of various autophagy-modulating drugs in CRC patients is needed to be explored. In this article, we present an overview of the autophagy process and its role in the pathogenesis and therapeutic resistance of CRC. Also, we focused on the current understanding of the role of the EMT and TME, including its relation to gut microbiota and immune cells, in autophagic manipulation of CRC. We believe that there is a potential link between autophagy, TME, EMT, and drug resistance, suggesting that further studies are needed to explore this aspect.</p

Calcium signaling in plant defense (involvement of subcellular compartments and glutamate receptors)

Les plantes présentent une forme d immunité innée face à des agents potentiellement pathogènes qui se traduit par l induction de réponses de défense. Les réponses immunes des plantes sont induites après détection de motifs moléculaires associés à des pathogènes ou à des micro-organismes par des récepteurs reconnaissant spécifiquement ces motifs et/ou des molécules dérivées des agents pathogènes ou de la plante, appelés éliciteurs de réaction de défense. La cryptogéine (Cry) et les oligogalacturonates (OGs) sont des éliciteurs établis de réactions de défense et leur reconnaissance induit une signalisation Ca2+-dépendante : un influx calcique et une variation de la concentration cytosolique en Ca2+ libre ([Ca]cyt) sont des événements précoces induisant une voie de signalisation de défense. Nous avons démontré que chez le tabac, les éliciteurs induisent une signalisation calcique dans les mitochondries et les chloroplastes. Des études pharmacologiques indiquent que des canaux IP3-dépendants régulent la signalisation calcique induite par la Cry dans les mitochondries et les chloroplastes. La respiration mitochondriale et les mécanismes de dissipation de l énergie dans les chloroplastes sont régulés en partie par la [Ca2+] dans ces organites. De plus, nous montrons par des approches pharmacologiques et génétiques, que des homologues aux récepteurs du glutamate (GLRs) participent à la signalisation calcique induite par les OGs dans Arabidopsis. Les GLRs contrôlent en partie la production d oxyde nitrique (NO) et d espèces réactives de l oxygène (ROS), ainsi que l expression de gènes de défense. Par ailleurs, les plantes traitées par des antagonistes des GLRs, présentent une moindre résistance au pathogène fongique nécrotrophique, Botrytis cinerea et à l oomycète biotrophique, Hyaloperonospora arabidopsidis. L analyse de mutants Atglr révèle l importante contribution de AtGLR3.3 dans la résistance envers H. arabidopsidis. De plus, de frappantes similarités dans l expression de gènes sont observées après traitement par les OGs ou après infection par H. arabidopsidis. Enfin, une analyse transcriptomique montre qu environ 60 % des gènes modulés par les OGs ont une expression qui dépend de GLRs. Ces gènes dépendants de GLRs appartiennent à diverses familles fonctionnelles dont celle répondant aux stress biotiques. En conclusion, ces études montrent 1) que les mitochondries et les chloroplastes présentent aussi une signalisation calcique induite par des éliciteurs de réaction de défense chez le tabac et 2) l implication de GLRs dans la signalisation calcique induite par des éliciteurs ou des agents pathogènes et la résistance envers des agents pathogènes chez ArabidopsisPlants do not display an adaptive immune system but express an efficient innate immune system defending them by inducing sophisticated multilevel defense responses against different potential pathogens. Indeed, plant immune responses are triggered upon the detection of many common pathogen- or microbe-associated molecular patterns (PAMPs/MAMPs) through specific pattern-recognition receptors (PRRs) and/or pathogen- or plant-derived signal molecules called elicitors. Cryptogein (Cry) and oligogalacturonides (OGs) are well known elicitors of defense reactions and their recognition induce a Ca2+-dependent signaling pathway: Ca2+ influx and subsequent free cytosolic [Ca2+] ([Ca2+]cyt) variations are earliest steps to trigger downstream plant defense signaling. Here we have demonstrated that elicitor-induced Ca2+ signaling in tobacco also takes place in mitochondria and chloroplasts. Pharmacological studies indicated that IP3-channels play an important role in the regulation of Ca2+ signaling in mitochondria and chloroplasts. Mitochondrial respiration and energy dissipation mechanisms in chloroplasts are partly controlled by [Ca2+] in these organelles. Moreover, using pharmacological and genetic approaches, our data demonstrated that glutamate receptors homologs (GLRs) participate in OGs-mediated Ca2+ signaling in Arabidopsis. GLRs partly control OGs-induced nitric oxide (NO) production, reactive oxygen species (ROS) production and expression of defense-related genes. Importantly, plants treated with GLRs antagonists exhibited compromised resistance to necrotrophic fungal pathogen, Botrytis cinerea and biotrophic oomycete, Hyaloperonospora arabidopsidis. Analysis of Atglr single mutants revealed the important contribution of AtGLR3.3 in resistance against H. arabidopsidis. Moreover, striking similarities in gene expression levels were observed after OGs elicitation/H. arabidopsidis infection. Finally, transcriptomic analysis demonstrated that about 60 % of the total OGs-modulated genes modified their expression in GLRs-dependent manner. These GLRs-dependent genes belong to different functional categories including the category responses to biotic stresses . Taken together, these data provide strong evidences of 1) elicitor-induced Ca2+ signaling in mitochondria and chloroplasts in tobacco and 2) the regulation of elicitor/pathogen mediated plant defense signaling pathways through GLRs in Arabidopsis thalianaDIJON-BU Doc.électronique (212319901) / SudocSudocFranceF

MLH1 mediates cytoprotective nucleophagy to resist 5-Fluorouracil-induced cell death in colorectal carcinoma

Colorectal Cancer (CRC) with Microsatellite instability (MSI) and mutLhomolog-1 (MLH1) gene deficiency are less aggressive than MLH1 proficient cancers. MLH1 is involved in several cellular processes, but its connection with the autophagy-dependent cellular response towards anticancer drugs remains unclear. In this study, we aimed to investigate the interaction between MLH1 and the autophagy marker LC3, which facilitated nucleophagy induction, and its potential role in determining sensitivity to 5-Fluorouracil (5-FU) induced cell death. To examine the role of MLH1 in DNA-damage-induced nucleophagy in CRC cells, we utilized a panel of MLH1 deficient and MLH1 proficient CRC cell lines. We included a parental HCT116 cell line (MLH1−/−) and its isogenic cell line HCT116 MLH1+/− in which a single allele of the MLH1 gene was introduced using CRISPR-Cas9 gene editing. We observed that MLH1 proficient cells were less sensitive to the 5-FU-induced cytotoxic effect. The 5-FU induced DNA damage led to LC3 up-regulation, which was dependent on MLH1 overexpression. Moreover, immunofluorescence and immunoprecipitation data showed LC3 and MLH1 were colocalized in CRC cells. Consequently, MLH1 dependent 5-FU-induced DNA damage contributed to the formation of micronuclei. These micronuclei colocalize with autolysosome, indicating a cytoprotective role of MLH1 dependent nucleophagy. Interestingly, siRNA knockdown of MLH1 in HCT116 MLH1+/− prevented LC3 upregulation and micronuclei formation. These novel data are the first to show an essential role of MLH1 in mediating the chemoresistance and survival of cancer cells by increasing the LC3 expression and inducing nucleophagy in 5-FU treated CRC cells.</p

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Bromelain is a concoction of sulfhydryl proteolytic enzymes. Depending upon the site of extraction it can be regarded as either stem bromelain (SBM) (EC 3.4.22.32) or fruit bromelain (FBM) (EC 3.4.22.33). Bromelain remain enzymatic active over a broad spectrumand endure a range of pH (5.5 to 8.0) and temperature (35.5 to 71 ºC). It is one of the extensively investigated proteolytic enzyme owing to its astonishing applications in various industries. This necessitated employing a strategy that result in highest purified bromelain in less steps and lowest cost. Use of modernistic approach such as membrane filtration, reverse micellar systems, aqueous two phase extraction and chromatographic techniques have shown promise in this regard. Besides its industrial applications, bromelain has been widely utilized as a potential phytomedical compound. Some of its reported actions include inhibition of platelet aggregation, anti-edematous, anti-thrombotic, anti-inflammatory, modulation of cytokines and immunity, skin debridement and fibrinolytic activity. It also assist digestion, enhance absorption of other drugs and is a potential postoperatively agent that promote wound healing and reduce postsurgical discomfort and swelling

Effect of accumulated Stomach Gas due to Positive Pressure Ventilation in Children on Oximetery Values

stomach gas in children, undergoing positive pressureventilation with face mask, helps in improving the arterialoxygen saturation. Methods: This was a quasi-experimental studywhose procedures were performed at Combined MilitaryHospital, Lahore and Mardan, from May 2005 to Nov 2006.Fifty children scheduled for surgery were included. Agerange of patients was between 06 months to 02 years.Patient status was American Society of AnaesthesiologistsI and II. All were ‘nil by mouth’ for the past 4-6 hours.In 25 cases undergoing positive pressure ventilation, thestomach gas was removed by nasogastric tube (Group A).In the other 25 cases the stomach gas was not removed(Group B). Oximetery was done during the conduct ofanaesthesia. Average value of each case was determinedand the data compared and analyzed. Results: 80% of patients in whom stomach gas wasremoved showed 100% oxygen saturation (SPO2) and theremaining 20% patients had 99% saturation. Whereas thegroup in which stomach gas was not removed, 48%patients showed 100% saturation, 32% patients had 99%and 20% patients had 98% oxygen saturation. Conclusion: Positive pressure ventilation with facemask in children can cause gaseous distension ofthe stomach. Removal of this gas can help improve theoxygen saturation

Age and site of Colonic Neoplastic Lesions: Implications of screening in South Asia.

Objective : To evaluate the Age of patients and the site of Colonic Neoplastic Lesions (CNL) and to determine the appropriate screening strategy for Colorectal Carcinoma (CRC) (sigmoidoscopy versus colonoscopy) in our population. Methods : This is a cross sectional study. Data of all patients more than 16 years of age who underwent full colonoscopic examination at the Aga Khan University hospital between January 2011 till December 2013 and were diagnosed to have CRC or advanced adenomas (defined as polyp more than 1 cm and/or having villous morphology on histology) was recorded. Lesions found distal to the splenic flexure were characterized as distal lesions and while lesions found between the splenic flexure and the cecum were characterized as proximal lesions. RESULTS: During the study period colonic neoplastic lesions were found in 217 patients; 186 (85.7%) patients had CRC and 31(14.3%) patients had advanced adenomatous polyps. Mean age was 55.8±14 years and amongst them 72 (33.2%) patients were less than 50 years of age while 145 (66.8%) were more than 50 years. In 144 (66.4%) patients lesions were located in the distal colon, 65 (30%) had lesions in the proximal colon while in 8 (3.7%) patients the neoplastic lesions were found both in the proximal and distal colon. The predominant symptoms were bleeding per rectum in 39.6% of patients followed by weight loss in 31.8% of patients. Only 3 patients had familial syndromes with multiple polyps. When patients younger than 50 years of age were compared with patients more than 50 years there was no statistically significant difference between the site of neoplastic lesion as well as the presenting symptoms. (p value 0.85). CONCLUSION: Colonic Neoplastic Lesions presented at younger age in our study population and one third of the lesions were found in the right sided colon. Hence screening for CNLs should be implied at an earlier age preferably with colonoscopy. More population based data is required to further validate our results