Interaction between SIRT1 and non-coding RNAs in different disorders

SIRT1 is a member of the sirtuin family functioning in the process of removal of acetyl groups from different proteins. This protein has several biological functions and is involved in the pathogenesis of metabolic diseases, malignancy, aging, neurodegenerative disorders and inflammation. Several long non-coding RNAs (lncRNAs), microRNAs (miRNAs) and circular RNAs (circRNAs) have been found to interact with SIRT1. These interactions have been assessed in the contexts of sepsis, cardiomyopathy, heart failure, non-alcoholic fatty liver disease, chronic hepatitis, cardiac fibrosis, myocardial ischemia/reperfusion injury, diabetes, ischemic stroke, immune-related disorders and cancers. Notably, SIRT1-interacting non-coding RNAs have been found to interact with each other. Several circRNA/miRNA and lncRNA/miRNA pairs that interact with SIRT1 have been identified. These axes are potential targets for design of novel therapies for different disorders. In the current review, we summarize the interactions between three classes of non-coding RNAs and SIRT1

Interplay between programmed death-ligand 1 and non-coding RNAs

Programmed death-ligand 1 (PD-L1) is a transmembrane protein with essential roles in the suppression of adaptive immune responses. As an immune checkpoint molecule, PD-L1 can be exploited by cancer cells to evade the anti-tumor attacks initiated by the immune system. Thus, blockade of the PD1/PD-L1 axis can eliminate the suppressive signals and release the antitumor immune responses. Identification of the underlying mechanisms of modulation of the activity of the PD1/PD-L1 axis would facilitate the design of more efficacious therapeutic options and better assignment of patients for each option. Recent studies have confirmed the interactions between miRNAs/lncRNAs/circ-RNAs and the PD1/PD-L1 axis. In the current review, we give a summary of interactions between these transcripts and PD-L1 in the context of cancer. We also overview the consequences of these interactions in the determination of the response of patients to anti-cancer drugs

Implication of non-coding RNA-mediated ROCK1 regulation in various diseases

Rho Associated Coiled-Coil Containing Protein Kinase 1 (ROCK1) is a protein serine/threonine kinase which is activated upon binding with the GTP-bound form of Rho. This protein can modulate actin-myosin contraction and stability. Moreover, it has a crucial role in the regulation of cell polarity. Therefore, it participates in modulation of cell morphology, regulation of expression of genes, cell proliferation and differentiation, apoptotic processes as well as oncogenic processes. Recent studies have highlighted interactions between ROCK1 and several non-coding RNAs, namely microRNAs, circular RNAs and long non-coding RNAs. Such interactions can be a target of medications. In fact, it seems that the interactions are implicated in therapeutic response to several medications. In the current review, we aimed to explain the impact of these interactions in the pathoetiology of cancers as well as non-malignant disorders

Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats

Objective(s): The physical properties of nerve guidance channel and components of the regenerating microenvironment can significantly enhance regeneration. The aim of this research was to evaluate the effect of embryo cerebrospinal fluid (ECSF) in nerve regeneration across the microwave irradiated collagen nerve guides in comparison with autograft. Material and Methods: Under general anesthesia, the left sciatic nerve was exposed and 10 mm nerve segment defect was created in 40 adult male Sprague-Dawley rats (250-300 g). Animals were randomly divided into 4 experimental groups: repair with reversed autograft, reconstruction with collagen nerve conduit filled with ECSF, reconstruction with collagen nerve conduit filled with normal saline (NS) and sham surgery. All animals were evaluated by sciatic functional index (SFI), electrophysiology, and histopathological staining at weeks 4 and 12 after surgery. Results: The mean SFI value of group collagen + ECSF and autograft was significantly higher than that of group NS on days 49 and 60 post-operation (P < 0.05). After 90 days after the operation, the mean nerve conduction velocity (NCV) of groups collagen + ECSF and autograft were significantly faster than NS group (P < 0.05). The regenerated nerves of groups collagen + ECSF and autograft were more mature than that of the group NS group at day 90 (P < 0.05). There was no significant difference between groups collagen + ECSF and autograft. Conclusion: These findings showed that chick CSF in collagen guide can enhance nerve regeneration and promote functional recovery in the injured sciatic nerve of rats

NLRP3: Role in ischemia/reperfusion injuries

NLR family pyrin domain containing 3 (NLRP3) is expressed in immune cells, especially in dendritic cells and macrophages and acts as a constituent of the inflammasome. This protein acts as a pattern recognition receptor identifying pathogen-associated molecular patterns. In addition to recognition of pathogen-associated molecular patterns, it recognizes damage-associated molecular patterns. Triggering of NLRP3 inflammasome by molecules ATP released from injured cells results in the activation of the inflammatory cytokines IL-1β and IL-18. Abnormal activation of NLRP3 inflammasome has been demonstrated to stimulate inflammatory or metabolic diseases. Thus, NLRP3 is regarded as a proper target for decreasing activity of NLRP3 inflammasome. Recent studies have also shown abnormal activity of NLRP3 in ischemia/reperfusion (I/R) injuries. In the current review, we have focused on the role of this protein in I/R injuries in the gastrointestinal, neurovascular and cardiovascular systems

Protective Effect of Galega officinalis Extract on Streptozotocin-Induced Kidney Damage and Biochemical Factor in Diabetic Rats

Objective: Diabetes mellitus (DM) is impairing secretion of insulin or resistance to insulin. Herbal medicine plays an important role in the management of DM. We aimed to test antidiabetic effects of Galega officinalis on diabetic rats. Materials and Methods: Twenty-eight male Wistar rats were randomly divided into 4 groups (n=7). Diabetes was induced by streptozotocin (STZ) (50 mg/kg). Diabetic rats were calcified into a diabetic control group (DC), DHEG group (50 mg/kg hydroalcoholic extract of G. officinalis), DG group (5 mg/kg glibenclamide). After 20 days, rats" blood samples, kidney, liver, and pancreas were kept in -70°C to test blood levels of glucose, insulin, lipid profile, some oxidative stress markers and antioxidant enzymes. Results: The fasting blood sugar (FBS) levels in the normal, DHEG, and DG groups were significantly lower than the DC group (P < 0.05); The levels of insulin in the DC, DHEG, and DG groups were significantly lower than the normal group (P < 0.05); The serum level of urea and creatinine was significantly increased in DC group and significantly decreased in other group (P < 0.05). Diabetes causes degenerative damages in rats kidney and treatment with G. officinalis extract protected kidney tissue against diabetes-induced damages. Conclusions: The results of the present study indicated that G. officinalis could be beneficial for the treatment of diabetes through improving tissue sensitivity to insulin and preventing tissue damages

Role of Long Non-Coding RNAs in Conferring Resistance in Tumors of the Nervous System

Tumors of the nervous system can be originated from several locations. They mostly have high mortality and morbidity rate. The emergence of resistance to chemotherapeutic agents is a hurdle in the treatment of patients. Long non-coding RNAs (lncRNAs) have been shown to influence the response of glioblastoma/glioma and neuroblastoma to chemotherapeutic agents. MALAT1, NEAT1, and H19 are among lncRNAs that affect the response of glioma/glioblastoma to chemotherapy. As well as that, NORAD, SNHG7, and SNHG16 have been shown to be involved in conferring this phenotype in neuroblastoma. Prior identification of expression amounts of certain lncRNAs would help in the better design of therapeutic regimens. In the current manuscript, we summarize the impact of lncRNAs on chemoresistance in glioma/glioblastoma and neuroblastoma