Biological reserves Rare Species and the Opportunity Cost of Diversity

The preservation of species diversity generally suggests protection of either the greatest number of species possible or all species. Requiring representation of each species in at least one parcel in the system and seeking the minimum number of parcels in the reserve system to achieve this requirement is termed the Species Set Covering Problem (SSCP). Nonetheless, it is important, as well, to consider the rarest of species, as their populations are the most in need of protection to assure their survival. This paper uses zero-one programming models and an existing data set to study species protection, rarity and the opportunity costs of diversity. We employ for this purpose an integer programming model that uses the SSCP format to require at least one representation of each and every species, but that seeks in addition protection of the rarest species. This is achieved by maximizing redundant coverage of those species designated as rare. Results are then compared to those of the SSCP. Recognizing that resources available for conservation purchases could well be insufficient to represent all species at least once, we structure a model aimed at trading-off first coverage of the greatest number of species against redundant coverage of rare species. We develop a tradeoff curve for this multi-objective problem in order to evaluate the opportunity cost of covering more species as redundant coverage of rare species decreases ­and vice versa. Finally, various possible rarity sets and various budget proxies are considered along with their impacts on conservation policies, Pareto optimality and on the opportunity cost of diversity

Phenotypic characterization and 16S rDNA identification of culturable non-obligate halophilic bacterial communities from a hypersaline lake, La Sal del Rey, in extreme South Texas (USA)

Background: La Sal del Rey ( the King’s Salt”) is one of several naturally-occurring salt lakes in Hidalgo County, Texas and is part of the Lower Rio Grande Valley National Wildlife Refuge. The research objective was to isolate and characterize halophilic microorganisms from La Sal del Rey. Water samples were collected from the lake and a small creek that feeds into the lake. Soil samples were collected from land adjacent to the water sample locations. Sample salinity was determined using a refractometer. Samples were diluted and cultured on a synthetic saline medium to grow halophilic bacteria. The density of halophiles was estimated by viable plate counts. A collection of isolates was selected, gram-stained, tested for catalase, and characterized using API 20E® test strips. Isolates were putatively identified by sequencing the 16S rDNA. Carbon source utilization by the microbial community from each sample site was examined using EcoPlate™ assays and the carbon utilization total activity of the community was determined. Results: Results showed that salinity ranged from 4 parts per thousand (ppt) at the lake water source to 420 ppt in water samples taken just along the lake shore. The density of halophilic bacteria in water samples ranged from 1.2 × 102 - 5.2 × 103 colony forming units per ml (cfu ml-1) whereas the density in soil samples ranged from 4.0 × 105 - 2.5 × 106 colony forming units per gram (cfu g-1). In general, as salinity increased the density of the bacterial community decreased. Microbial communities from water and soil samples were able to utilize 12 - 31 carbon substrates. The greatest number of substrates utilized was by water-borne communities compared to soil-based communities, especially at lower salinities. The majority of bacteria isolated were gram-negative, catalase-positive, rods. Biochemical profiles constructed from API 20E® test strips showed that bacterial isolates from low-salinity water samples (4 ppt) showed the greatest phenotypic diversity with regards to the types and number of positive tests from the strip. Isolates taken from water samples at the highest salinity (420 ppt) tended to be less diverse and have only a limited number of positive tests. Sequencing of 16S DNA displayed the presence of members of bacterial genera Bacillus, Halomonas, Pseudomonas, Exiguobacterium and others. The genus Bacillus was most commonly identified. None of the isolates were members of the Archaea probably due to dilution of salts in the samples. Conclusions: The La Sal del Rey ecosystem supports a robust and diverse bacterial community despite the high salinity of the lake and soil. However, salinity does appear to a limiting factor with

Exploring light dark matter with the DarkSPHERE spherical proportional counter electroformed underground at the Boulby Underground Laboratory

We present the conceptual design and the physics potential of DarkSPHERE, a proposed 3 m in diameter spherical proportional counter electroformed underground at the Boulby Underground Laboratory. This effort builds on the R&D performed and experience acquired by the NEWS-G Collaboration. DarkSPHERE is primarily designed to search for nuclear recoils from light dark matter in the 0.05--10 GeV mass range. Electroforming the spherical shell and the implementation of a shield based on pure water ensures a background level below 0.01 dru. These, combined with the proposed helium-isobutane gas mixture, will provide sensitivity to the spin-independent nucleon cross-section of $2\times 10^{-41} (2\times 10^{-43})$ cm$^2$ for a dark matter mass of $0.1 (1)$ GeV. The use of a hydrogen-rich gas mixture with a natural abundance of $^{13}$C provides sensitivity to spin-dependent nucleon cross-sections more than two orders of magnitude below existing constraints for dark matter lighter than 1 GeV. The characteristics of the detector also make it suitable for searches of other dark matter signatures, including scattering of MeV-scale dark matter with electrons, and super-heavy dark matter with masses around the Planck scale that leave extended ionisation tracks in the detector.Comment: 19 pages, 14 figure

Biology of moderately halophilic aerobic bacteria

The moderately halophilic heterotrophic aerobic bacteria form a diverse group of microorganisms. The property of halophilism is widespread within the bacterial domain. Bacterial halophiles are abundant in environments such as salt lakes, saline soils, and salted food products. Most species keep their intracellular ionic concentrations at low levels while synthesizing or accumulating organic solutes to provide osmotic equilibrium of the cytoplasm with the surrounding medium. Complex mechanisms of adjustment of the intracellular environments and the properties of the cytoplasmic membrane enable rapid adaptation to changes in the salt concentration of the environment. Approaches to the study of genetic processes have recently been developed for several moderate halophiles, opening the way toward an understanding of haloadaptation at the molecular level. The new information obtained is also expected to contribute to the development of novel biotechnological uses for these organisms

Observation of the formation of an optical intensity shock and wave breaking in the nonlinear propagation of pulses in optical fibers

We have observed the formation of an optical intensity shock and the subsequent wave breaking in the nonlinear propagation of 1-psec pulses in an optical fiber. The wave breaking manifests itself as the appearance of oscillations trailing the shock, which are due to the beating of widely separated frequency components which bridge the shock. The experimental results are in good agreement with numerical solutions of the nonlinear Schrodinger equation.Peer reviewedElectrical and Computer Engineerin

Biological Reserves, Rare Species and the Tradeoff Between Species Abundance and Species Diversity

The preservation of species diversity generally suggests protection of either the greatest number of species possible or all species. Requiring representation of each species in at least one parcel in the system and seeking the minimum number of parcels in the reserve system to achieve this requirement is termed the Species Set Covering Problem (SSCP). Nonetheless, it is important, as well, to consider the rarest of species, as their populations are the most in need of protection to assure their survival. This paper uses 0–1 programming models and an existing data set to study species protection, rarity, species abundance and species diversity. We employ for this purpose an integer programming model that uses the SSCP format to require at least one representation of each and every species, but that seeks in addition protection of the rarest species. This is achieved by maximizing redundant coverage of those species designated as rare. Results are then compared to those of the SSCP. Recognizing that resources available for conservation purchases could well be insufficient to represent all species at least once, we structure a model comparing coverage of the greatest number of species and redundant coverage of rare species. We develop a trade-off curve for this multi-objective problem in order to evaluate the opportunity cost of covering more species as redundant coverage of rare species decreases—and vice versa. Finally, various possible rarity sets and various budget proxies are considered along with their impacts on conservation policies, Pareto optimality and species diversity

An all solid-state polymer-polymer electrolyte-lithium rechargeable battery for room temperature applications

A new polymer electrolyte based on polyethylene oxide (PEO) and styrenic macromonomer of PEO-lithium perchlorate complexes, conceived for room-temperature battery applications, has been tested in a lithium polybithiophene rechargeable battery. Cyclability and stability data are reported and discussed. © 1990

New approaches in hydrogel synthesis -- Click chemistry: A review

International audienceHydrogels have attracted great attention over the past decades, since they can be used for a variety of applications, including drug delivery systems and scaffolds for tissue engineering and repair. "Click chemistry", in particular copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), has been widely used in the preparation of complex architectures, such as hydrogel networks, due to its reaction specificity, quantitative yields and good functional group tolerance. The aim of this review is to present the synthesis and further use of "click" hydrogels composed of primarily natural components, using Cu as a catalyst or, following the latest trends, via a copper-free method