Effect of Black Pepper Extract (Piper nigrum L) towards Streptococcus Mutans Growth: Pengaruh Ekstrak Lada Hitam (Piper nigrum L) terhadap Pertumbuhan Streptococcus Mutans

Streptococcus mutans is a Gram-positive facultative anaerobic bacteria which has become the main cause of dental caries. Black pepper (Piper nigrum L ) is one of herbal plants that compose antibacterial compounds including alkaloids, flavonoids, tannins, and essential oils. This study aims to determine the effect of black pepper (Piper nigrum L.) extract to inhibit of Streptococcus mutansgrowth. Black pepper extracts ware made by using maceration method. The effect of black pepper extract ( Piper nigrum L. ) in inhibition of Streptococcus mutansgrowth by using the disc diffusion method on Mueller Hinton Agar media. The concentration of black pepper ( Piper nigrum L.) extracts used in this study were 6.25%, 12.5%, 25%, 50%, 75%. The results of this study were analyzed by using the one-way ANOVA test showed that there was significant effect of black pepper extract on Streptococcus mutans growth with p-value (p <0.05), and then the data was tested by Least Significant Difference (LSD). Conclusion of this study showed that there was an effect of black pepper ( Piper nigrum L.) extract in inhibition of Streptococcus mutans growth with concentrations 75% is the optimum concentration which value 16.8 mm

Comparison of the effect of guava leaf extract (Psidium guajava L.) with polyvinylpyrrolidone-sodium hyaluronate on the number fibroblasts (study on white rats [Rattus norvegicus])

Background: Wound healing is a cellular and complex process that aims to restore the integrity of tissue damaged by injury. The phases that occur in the wound healing process are inflammation, proliferation, and maturation (remodeling). One of the final results of wound healing is fibroblasts proliferation. Fibroblasts have the ability to synthesize collagen fibeBackground: Wound healing is a cellular and complex process that aims to restore the integrity of tissue damaged by injury. The phases that occur in the wound healing process are inflammation, proliferation, and maturation (remodeling). One of the final results of wound healing is fibroblasts proliferation. Fibroblasts have the ability to synthesize collagen fibers that will cover the injured area. Natural medicinal plants that have the potential to accelerate wound healing are guava (Psidium guajava L.) leaves which contain flavonoids, tannins, saponins, and alkaloids. Polyvinylpyrrolidone-sodium hyaluronate gel is one of the wound treatment products on the market and can help the wound healing process Methods: This research method was an experimental laboratory using 10 white rat samples which were divided into two groups and given injury to the mandibular anterior gingiva. Both treatment groups were applied topically in the morning and evening for 14 days, then the tissue was observed histologically. Results: Observation of the number of fibroblasts in the treatment group applied to guava leaf extract gel had a mean of 28±11 while the treatment group applied Polyvinylpyrrolidone-sodium hyaluronate gel was 10±4. The unpaired t test (Independent T-Test) showed a significance value of 0.000 (

PEMANFAATAN BAHAN ALAMI SEBAGAI UPAYA PENGHAMBAT Candida albicans PADA RONGGA MULUT

Candida albicans merupakan salah satu flora normal yang keberadaannya paling banyak terdapat pada rongga mulut, kulit, membran mukosa, saluran pencernaan, saluran pernapasan dan vagina. Penyakit yang disebabkan oleh Candida albicans yaitu penyakit kandidiasis oral (moniliasis). Penggunaan bahan obat-obatan dalam jangka waktu yang lama dapat menimbulkan resistensi fungi terhadap obat dan beberapa obat memiliki efek negatif. Oleh karena itu berbagai penelitian telah dilakukan untuk mencari alternativ lain sumber obat yang mampu menghambat pertumbuhan Candida albicans. Umumnya penelitian melaporkan bahwa senyawa-senyawa yang terkandung pada tumbuhan memiliki sifat anti-fungi terhadap Candida albicans. Senyawa/ zat aktif tersebut adalah flavonoid, tanin, saponin, alkaloid, polifenol, triterpenoid dan beberapa komponen aktif seperti alpha-mangostin

Peran matriks metalloproteinase pada kerusakan jarinngan periodontal akibat respon imun host terhadap periodontitis

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Peran gen P53 sebagai gen penekan tumor pada kanker rongga mulut

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Perbandingan respon hambat ekstrak lengkuas rimpang putih (Alpina galanga) 10% dengan ekstrak lengkuas rimpang merah (Alpina purpurata) 10 % terhadap pertumbuhan Candida albicans

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Identifikasi dan Uji Sensitivitas Bakteri dari Penderita Abses Odontogenik di Blu RSU Meuraxa Banda Aceh

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Inhibition of 10% Alpinia galanga and Alpinia purpurata rhizome extract on Candida albicans growth

Background: One of normal oral flora that found in human oral cavity is Candida albicans (C. albicans). The overgrowth of this species can lead to opportunistic infection known as candidiasis. Two natural plants, Alpinia galanga rhizome and Alpinia purpurata rhizome, are natural remedies containing flavonoid, saponin, tannin, and triterpenoid used as antifungal component. Purpose: This experimental laboratory study is aimed to determine the inhibition of Alpinia galanga rhizome and Apinia purpurata rhizome on the growth of C. albicans. Methods: Alpinia galanga rhizome and Alpinia purpurata rhizome were extracted in ethanol solvent using soxhletation method. The ratio test was conducted on those two extracts at the concentration of 10% toward the growth of C. albicans through agar diffusion method. Results: The results showed that 10% Alpinia galanga rhizome extract and 10% Alpinia purpurata rhizome extract were able to inhibit the growth of C. albicans, about 7.33 mm for Alpinia galanga rhizome extract and 6 mm for Alpinia purpurata rhizome extract. The results of statistical tests using independent samples t-test showed that there was no significant difference between the inhibition of 10% Alpinia galanga rhizome extract and that of 10% Alpinia purpurata rhizome extract. Conclusion: In conclusion 10% Alpinia galanga rhizome extract and 10% Alpinia purpurata rhizome extract have weak inhibition on C. albicans growth.Latar belakang: Candida albicans (C. albicans) merupakan flora normal yang terdapat dalam rongga mulut, jika keseimbangannya terganggu maka jamur tersebut akan menjadi patogen dan dapat menyebabkan infeksi dalam rongga mulut yaitu kandidiasis. Lengkuas rimpang putih maupun lengkuas rimpang merah merupakan tanaman yang mengandung senyawa antijamur berupa flavonoid, saponin, tanin, dan triterpenoid. Tujuan: Penelitian eksperimental laboratoris ini dilakukan untuk mengetahui perbandingan daya hambat ekstrak lengkuas rimpang putih (Alpinia galanga) dengan ekstrak lengkuas rimpang merah (Alpinia purpurata) terhadap pertumbuhan C. albicans. Metode: Ekstrak lengkuas rimpang putih maupun lengkuas rimpang merah diperoleh dengan metode soxhletasi. Dilakukan pengujian perbandingan kedua ekstrak pada konsentrasi 10% terhadap pertumbuhan C. albicans dengan menggunakan metode difusi agar. Hasil: Ekstrak lengkuas rimpang putih 10% dan ekstrak rimpang lengkuas merah 10% mampu menghambat pertumbuhan C. albicans dengan daya hambat rata 7,33 mm untuk ekstrak lengkuas rimpang putih dan 6 mm untuk ekstrak lengkuas rimpang merah 10%. Hasil uji statistik menggunakan independent sampel t test menunjukkan tidak ada perbedaan bermakna antara respon hambat ekstrak lengkuas rimpang putih dan ekstrak lengkuas rimpang merah. Kesimpulan: Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak lengkuas rimpang putih 10% dan ekstrak lengkuas rimpang merah 10% memiliki daya hambat yang lemah terhadap pertumbuhan C. albicans.</p

EFFECT OF CORAL GONIOPORA IN COMPARISON WITH CORAL APATITE TOWARDS HUMAN DENTAL PULP STEM CELLS MINERALIZATION ACTIVITIES: EFEK CORAL GONIOPORA DIBANDINGKAN DENGAN CORAL APATITE TERHADAP AKTIVITAS MINERALISASI SEL STEM PULPA GIGI

Challenging approach in tissue engineering for dentin regeneration is focused upon the application of a scaffold on anopen pulp enabling odontoblast-like cells to grow into the scaffold and to convert them into dentin-like substance. Coralwas chosen as a scaffold because of its good biocompatibility and resorbability. The species of marine invertebratesexploited in medical applications are Members of Porites and Goniopora. Coral goniopora is most marine invertebratafound in Indonesia's marine. Coral apattite, an osteoconductive synthetic bone graft substitute material, is manufacturedby the hydrothermal conversion of the calcium carbonate skeleton of coral to hydroxyapattite in the presence ofammonium phosphate preserving the original porous structure which is similar to that of bone. The aim of study was toinvestigate the effect of Coral goniopora and coral apattite as a potential scaffold on dental pulp mineralization activity. Invitro DPSCs mineralization activity was measured by von Kossa staining for calcium deposit identification. The resultthat Coral apattite increased more calcium deposited identification than coral goniopora. Calcium deposited on dentalpulp stem cells are marker for mineralized dental pulp stem cells (DPSCs). Mineralized DPSCs are marker forodontoblast diferentiation and maturation. In conclusion, these observations demonstrated that co-cultured coral apattiteand DPSCs induced a better mineralization activity than those cultured with Coral goniopora