Evaluation of prophylactic Effect of Remifentanil on Succinylcholine-Induced Myalgia in Humans

Purpose: To assess the efficacy of remifentanil in preventing succinylcholine-induced myalgia in humans.Methods: Sixty healthy adults scheduled for elective surgery under general anesthesia were enrolled in a double-blind study and randomly allocated to two groups of thirty patients. Patients in Group I (remifentanil group) were pretreated with remifentanil 1 ìg/kg one minute prior to induction of anesthesia, while patients in Group II (saline group) received an equivalent volume of saline. Anesthesia was induced in both groups with fentanyl 1 µg/kg, propofol 2.0 mg/kg and succinylcholine 1.5 mg/kg. Postoperative myalgia was assessed 12, 24, and 48 h after induction and graded as nil, mild, moderate, or severe.Results: Fifty nine patients completed the study. The demographic data for both groups were comparable (p > 0.05). Postoperative myalgia at 12, 24, and 48 h after induction were 34.5, 34.5 and 14.1 % in group I, and 60, 53.4 and 30 % in group II, respectively (p > 0.05).Conclusion: Prophylactic use of remifentanil 1 µg/kg intravenously is ineffective in the prevention of postoperative myalgiaKeywords: Remifentanil, Succinylcholine, Myalgia, Propofol, Prophylaxi

Inhibition of MicroRNA miR-222 with LNA Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia

MicroRNAs (miRNAs) are small regulatory molecules that negatively regulate gene expression by base-pairing with their target mRNAs. miRNAs have contribute significantly to cancer biology and recent studies have demonstrated the oncogenic or tumor-suppressing role in cancer cells. In many tumors up-regulation miRNAs has been reported especially miR-222 has been shown to be up-regulated in B chronic lymphocytic leukemia (B-CLL). In this study we assessed the effected inhibition of miR-222 in cell viability of B-CLL. We performed inhibition of mir-222 in B-CLL cell line (183-E95) using locked nucleic acid (LNA) antagomir. At different time points after LNA-anti-mir-222 transfection, miR-222 quantitation and cell viability were assessed by qRT-real time polymerase chain reaction and MTT assays. The data were analyzed by independent t test and one way ANOVA. Down-regulation of miR-222 in B-CLL cell line (183-E95) with LNA antagomir decreased cell viability in B-CLL. Cell viability gradually decreased over time as the viability of LNA-anti-mir transfected cells was <47 % of untreated cells at 72 h post-transfection. The difference in cell viability between LNA-anti-miR and control groups was statistically significant (p < 0.042). Based on our findings, the inhibition of miR-222 speculate represent a potential novel therapeutic approach for treatment of B-CLL

Immunofluorescent labeling of CD20 tumor marker with quantum dots for rapid and quantitative detection of diffuse large B-cell non-Hodgkin's lymphoma

Fluorescent semiconductor quantum dots (QDs) are newfound nanocrystal probes which have been used in bioimaging filed in recent years. The purpose of this study is to evaluate the diagnostic value of specific QDs coupled to rituximab monoclonal antibody against CD20 tumor markers for patients with diffuse large B-cell lymphoma (DLBCL). In current study rituximab-conjugated quantum dots (QDs-rituximab) were prepared against CD20 tumor markers for detection of CD20-positive cells (human Raji cell line) using flowcytometry. A total of 27 tumor tissue samples were collected from patients with DLBCL and 27 subjects with negative pathological tests as healthy ones, which stained by QD-rituximab. The detection signals were obtained from QDs using fluorescence microscopy. The flowcytometry results demonstrated a remarkable difference in fluorescent intensity and FL2-H + (CD20-positive cells percentage) between two groups. Both factors were significantly higher in Raji in comparison with K562 cell line (P < 0.05). Lot of green fluorescence signals was observed due to the selectively binding of QD-rituximab to CD20 tumor markers which overexpressed in tumor tissues and a few signals observed on the defined healthy ones. Based on these observations the cut-off point was 46.8 dots and the sensitivity, specificity, positive predictive value, and negative predictive value were 100, 89.5, 91.3, and 100, respectively (LR+, 9.52; LR�, 0). The QD -rituximab could be beneficial as a bioimaging tool with high sensitivity to provide an accurate molecular imaging technique for identifying CD20 tumor markers for early diagnosis of the patients with DLBCL. © 2018 Wiley Periodicals, Inc

Database of rocking shallow foundation performance: Slow-cyclic and monotonic loading

Many physical model tests have examined the performance of rocking foundations during cyclic and seismic loading. These tests varied in model size, testing equipment, superstructure properties, footing shape, supporting soil environment, and loading protocol. “FoRCy, Foundation Rocking database of Cyclic and Monotonic Loading” is a new database (published at https://datacenterhub.org/), summarizing the results of monotonic and slow-cyclic loading tests of rocking foundations. The database consists of columns identifying testing equipment and facility, soil, superstructure, and system properties, as well as loading protocol and results. The database contains 456 records (rows), each one being unique in either model configuration or loading amplitude. To illustrate its value, this article shows correlations between (1) settlement, rotation, and factor of safety with respect to bearing capacity and (2) moment and cumulative rotation for shallow footings. Data indicate that the rotation required to mobilize the moment capacity is surprisingly constant (about 0.01 radians) for a wide range of experiments

EERI Virtual Earthquake Reconnaissance Team (VERT): Phase 1 Response to M5.7 Magna Earthquake

The Earthquake Engineering Research Institute (EERI) Virtual Earthquake Reconnaissance Team (VERT) is a subcommittee of the Learning from Earthquakes Committee. VERT performs virtual reconnaissance after an earthquake to provide timely multi-disciplinary information to the reconnaissance community to assist in decisions being made after an event. This document is the Phase 1 report for the Magna Earthquake

EERI Virtual Earthquake Reconnaissance Team (VERT): Phase 1 Response to M7.4 Crucecita, Oaxaca, Mexico Earthquake

The Virtual Earthquake Reconnaissance Team (VERT) is a sub-committee of the Learning from Earthquakes (LFE) Committee of the Earthquake Engineering Research Institute (EERI). The VERT Phase 1 report summarizes damage due to an earthquake on a variety of topics including hospitals, roads, geotechnical damage, lifelines, and fires following earthquakes

EERI: Lugu, Taiwan September 18, 2022, Mw 6.9 Earthquake Preliminary Virtual Reconnaissance Report (PVRR)

On September 18th, 2022, at 2:44 pm local time, a moment magnitude 6.9 earthquake struck 42.7 km north of Taitung City, Taiwan. The epicentral coordinates reported by the U.S. Geological Survey and the Taiwanese Central Weather Bureau Seismological Center were 23.159°N 121.316°E and 23.14°N 121.20°E, respectively. The USGS reported a hypocenter depth of 10.0 km, whereas the Central Weather Bureau Seismological Center reported a depth of 7.0 km. The earthquake was preceded by a magnitude 6.5 earthquake in the same area 17 hours earlier. There have been several earthquakes of magnitude 5.0 and larger in the sequence. This earthquake caused some geotechnical failures and damage to infrastructure, mainly bridges, railroads, and highways. Damage to buildings was observed, but it was primarily concentrated on older non-ductile structures and non-structural component damage. At least one building collapsed and hundreds more had some damage. The earthquake resulted in at least one death and 171 earthquake related injuries. The earthquake highlighted the vulnerabilities of older non-ductile concrete buildings in seismic regions, concrete bridges, and non-structural building components during an earthquake. This event also highlighted the importance of an earthquake early warning system. The purpose of this Preliminary Virtual Reconnaissance Report (PVRR) is to provide a summary of the characteristics of the event, the seismicity of the impacted region, and an overview of the main effects of the earthquake as collected from publicly available information