\u3ci\u3eCamelina sativa\u3c/i\u3e promoters of seed development genes resemble \u3ci\u3eArabidopsis thaliana\u3c/i\u3e orthologs

Camelina sativa, a relative of the model plant Arabidopsis thaliana, has gained commercial interest in recent years for its seed oil. Camelina oil is a desirable ingredient in animal feed and for cooking due to its rich concentration of omega-3 fatty acids. It is also emerging as an eco-friendly jet biofuel. However, since C. sativa has only recently entered the public eye, regulation of its seed oil synthesis remains largely unexplored. A. thaliana, on the other hand, has been extensively studied. Promoters directly upstream of genes harbor hundreds of cis-acting regulatory DNA elements. These motifs are often tissue-specific and associated with specific biological processes. Many motifs are also induced by hormones, which regulate development. Comparing promoter motifs of four prominent seed development genes, FUS3, LEC1, LEC2, and ABI3, in A. thaliana to their orthologs in C. sativa revealed significant similarity between the two. This suggests that regulation of seed development in C. sativa may be similar to that of A. thaliana. Notably, seed-specific motifs associated with storage proteins and carbon metabolism are most highly represented in both species. However, C. sativa seed-specific promoters were found to harbor a greater composition of motifs induced by abscisic acid, which has been implicated in seed maturation and dormancy. Moving forward, expression analysis of these genes in various C. sativa tissue types will further validate their function. Understanding specific regulatory factors modulating seed development will reveal molecular targets to improve camelina oil yield for industrial applications

Cognitive Motivation for Unaccusative Verbs with Objects in Existential Construction in Chinese

This study applies conceptual blending and grammatical blending to analyze the meaning and structure construction of the NP1+Vi+NP2 construction in existential construction in Chinese. We found that the NP1+Vi+NP2 construction in existential sentences is the result of conceptual integration and grammatical blending of two subevents with basic grammatical structure of NP1+Vt+NP2 and NP2+Vi respectively. By discussing process of semantic construction and syntactic realization, we derive that the structure of existential sentences is integrated by the input spaces of "the existing object exists (or lies on some status)" and "somewhere exists something". It can concludes that the emergent meaning is "somewhere exists the existing object lying on some status" through analyzing the blending characters. This proves that conceptual integration and grammatical blending theories are animate and have mighty explanatory power in this specific linguistic phenomenon

Factors affecting physicians’ attitudes towards patient-centred care: a cross-sectional survey in Beijing

OBJECTIVE: Patient-centred care has been raised as an important component in providing high-quality healthcare services. This research aims to measure physicians’ attitudes towards patient-centred care in Chinese healthcare settings and to identify the sociodemographic predictors of their attitudes using an exploratory research design. DESIGN: A cross-sectional survey. SETTING: Twelve hospitals in Beijing, China. PARTICIPANTS: 1290 physicians from 12 hospitals in Beijing were invited to take part in the survey using snowball sampling methods. There was a response rate of 84% (n=1084), of which 1053 responses (82%) were valid and included in this research. METHODS: This research used a survey containing a previously validated 6-point Likert scale called ‘Chinese-revised Patient-Practitioner Orientation Scale’ (CR-PPOS). Descriptive statistics and multivariable logistic regression analyses were performed to measure participants’ attitudes and to identify the sociodemographic predictors of Chinese physicians’ attitudes towards patient-centred care. RESULTS: Gender, professional title (ie, seniority) and hospital type influence Chinese physicians’ attitudes towards patient-centred care. Female physicians, physicians with intermediate titles and those who work in tertiary (ie, top-level) hospitals tend to have higher patient-centred attitudes (OR=1.532, 95% CI 1.160 to 2.022; OR=2.089, 95% CI 1.206 to 3.618; OR=2.198, 95% CI 1.465 to 3.297) than male physicians with other titles, and than those who work in first, secondary or private hospitals. Physicians working in non-surgical departments, those who have received training in doctor–patient communication, and those who are satisfied with their income obtained high patient-centred scores, both on the overall CR-PPOS and its two subscales. CONCLUSIONS: This research identified sociodemographic predictors of Chinese physicians’ attitudes towards patient-centred care. The findings contribute to knowledge of factors to be considered in reforming medical education and the Chinese healthcare system to improve physician–patient relationships and provide high-quality healthcare to patients. However, these findings are exploratory in nature and require further investigation to establish their validity and generalisability

Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

Tumor necrosis factor receptor-associated factors (TRAFs) have been studied in a few mollusks and participate in various biological processes, like apoptosis, immune response, stress, and inflammatory response. However, TRAFs’ function and mechanism of pearl oysters (Pinctada fucata martensii) are still unclear. In this study, the novel PmTRAF2 and PmTRAF4 from P. f. martensii were cloned by rapid amplification of complementary DNA ends and their mRNA expression were analyzed by quantitative real-time PCR (qPCR). The interacting protein of PmTRAF2 was verified by the yeast two-hybrid assay. The result shows that full-length of PmTRAF2 and PmTRAF4 cDNA were 2055 bp and 2365 bp, respectively. The deduced PmTRAF2 and PmTRAF4 proteins contain TRAF-type zinc finger domain and MATH domain, while PmTRAF4 lacks a RING finger domain. Multiple sequence alignment revealed that PmTRAF2 and PmTRAF4 had high homology with the ortholog of other species. Phylogenic analysis indicated that PmTRAF4 clustered with the homolog protein of Mytilus edulis and Mytilus galloprovincialis, and PmTRAF2 has the closest genetic relationship to Crassostrea gigas TRAF2. The qPCR analysis revealed that PmTRAF2 and PmTRAF4 were expressed in all six tissues, and both of them were significantly expressed in hepatopancreas and gill (p < 0.01). Under lipopolysaccharide (LPS) stimulation, polyinosinic acid (PolyI:C) stimulation, and nucleus insertion surgery, the transcripts of PmTRAF2, PmTRAF3, PmTRAF4 and PmTRAF6 in hepatopancreas were markedly changed at corresponding time points. These results have indicated that these genes may play a role in P. f. martensii innate immunity. Yeast two-hybrid assays show that PmTRAF2 interacts with PmTRAF6 but not PmTRAF3, potentially affecting downstream immune signaling pathways. Our findings provide new perspectives for further investigation of TRAFs’ immune mechanisms in bivalves

Subcellular localization and function study of a secreted phospholipase C from Nocardia seriolae

Fish nocardiosis is a chronic systemic granulomatous disease, andNocardia seriolaeis the main pathogen that causes this disease. But the pathogenesis and virulence factors ofN. seriolaeare not fully understood. A phospholipase C (PLC), which was likely to be a secreted protein targeting host cell mitochondria, was found by the bioinformatics analysis on the whole genome sequence ofN. seriolae. In order to determine the subcellular localization and study the preliminary function of PLC fromN. seriolae(NsPLC), the gene cloning, secreted protein identification, subcellular localization in host cells and apoptosis detection of NsPLC were carried out in this study. The results showed that NsPLC was a secreted protein by mass spectrometry analysis of extracellular products fromN. seriolae. Subcellular localization of NsPLC-GFP fusion protein in FHM cells revealed that the green fluorescence exhibited a punctate distribution near the nucleus and did not co-localize with mitochondria. In addition, apoptosis assay suggested that apoptosis was induced in FHM cells by the overexpression of NsPLC. This study may lay the foundation for further study on the function of NsPLC and promote the understanding of the virulence factors and pathogenic mechanism ofN. seriolae

(Z)-N-{(E)-10-[(2,6-Diisopropyl­phen­yl)­imino]-9,10-dihydro­phenanthren-9-yl­idene}-2,6-dimethyl­aniline

The title compound, C34H34N2, adopts a Z,E configuration with respect to the N=C—C=N backbone, with an N—C—C—N torsion angle of 41.1 (4)° The dihedral angle between the benzene rings in the 9,10-dihydro­phenanthrene moiety is 18.0 (1)°

Poplar Allene Oxide Synthase 1 Gene Promoter Drives Rapid and Localized Expression by Wounding

Promoters play critical roles in controlling the transcription of genes and are important as tools to drive heterologous expression for biotechnological applications. In addition to core transcription factor-binding motifs that assist in the binding of RNA polymerases, there are specific nucleotide sequences in a promoter region to allow regulation of gene expression. The allene oxide synthase (AOS) gene family are cytochrome P450s that are responsive to a variety of environmental stress, making them good candidates for the discovery of inducible promoters. Populus AOS homologs separate phylogenetically into two clades. Based on the 19 promoter motifs with significant abundance differences between the two clades, Clade I AOS genes are likely more responsive to hormones, salt, and pathogen, whereas clade II homologs are likely inducible by water stress. In this study, an upstream promoter from a Clade I poplar AOS encoding gene (AOS1) was cloned and used to drive the expression of a ß-glucuronidase (GUS) gene in Arabidopsis. AOS is an essential enzyme in the lipoxygenase pathway that is responsible for the production of many nonvolatile oxylipins in plants, including the jasmonates, which are regulatory phytohormones coordinating a variety of biological and stress response functions. Consistent with AOS transcript expression patterns, we found that the poplar AOS1 promoter drives rapid and localized expression by wounding. The study provides insight on the responsive elements in the poplar AOS promoters, but more importantly identifies a strong wound-inducible and localized promoter for future applications. Key Message: Populus AOSs separate phylogenetically into two clades, which show significant abundance differences in 19 promoter motifs. * AOS1 is predominantly expressed in growing vascular tissue in Populus * Populus AOS1 promoter drives rapid and localized expression by wounding in Arabidopsis