278 research outputs found
Study on Deterioration Mechanism and Prevention and Curing Techniques of an RC Bunker
In the coal mining industrial environment, the materials of RC bunkers suffer from serious aging problems. The cracking and deterioration of concrete and the corrosion of steel reinforcement lead to a degeneration of structural performance and a decline of structural reliability. In this paper, based on on-site detection and coal mining ground industrial environment, the deterioration characteristics of RC bunkers were tested. The investigation involved the apparent characteristics, carbonized depth, compression strength of concrete, reinforcement distribution and cover thickness, corrosion rate and mechanical properties of reinforcement, decline degree of bunkers, and so on. Then a design review check was done. Combined with the above information, the cause and mechanisms of cracking and damage of the structure were studied; finally, the problems of RC bunkers were targeted and improved. The work provides a reference method for repairing a deteriorated RC bunker in an aggressive service environment
Construction of stable Ta3N5/g-C3N4 metal/non-metal nitride hybrids with enhanced visible-light photocatalysis
In this paper, a novel Ta3N5/g-C3N4 metal/non-metal nitride hybrid was successfully synthesized by a facile impregnation method. The photocatalytic activity of Ta3N5/g-C3N4 hybrid nitrides was evaluated by the degradation of organic dye rhodamine B (RhB) under visible light irradiation, and the result indicated that all Ta3N5/g-C3N4 samples exhibited distinctly enhanced photocatalytic activities for the degradation of RhB than pure g-C3N4. The optimal Ta3N5/g-C3N4 composite sample, with Ta3N5 mass ratio of 2%, demonstrated the highest photocatalytic activity, and its degradation rate constant was 2.71 times as high as that of pure g-C3N4. The enhanced photocatalytic activity of this Ta3N5/g-C3N4 metal/metal-free nitride was predominantly attributed to the synergistic effect which increased visible-light absorption and facilitated the efficient separation of photoinduced electrons and holes. The Ta3N5/g-C3N4 hybrid nitride exhibited excellent photostability and reusability. The possible mechanism for improved photocatalytic performance was proposed. Overall, this work may provide a facile way to synthesize the highly efficient metal/metal-free hybrid nitride photocatalysts with promising applications in environmental purification and energy conversion
Parameter Identification Method for SINS Initial Alignment under Inertial Frame
The performance of a strapdown inertial navigation system (SINS) largely depends on the accuracy and rapidness of the initial alignment. The conventional alignment method with parameter identification has been already applied widely, but it needs to calculate the gyroscope drifts through two-position method; then the time of initial alignment is greatly prolonged. For this issue, a novel self-alignment algorithm by parameter identification method under inertial frame for SINS is proposed in this paper. Firstly, this coarse alignment method using the gravity in the inertial frame as a reference is discussed to overcome the limit of dynamic disturbance on a rocking base and fulfill the requirement for the fine alignment. Secondly, the fine alignment method by parameter identification under inertial frame is formulated. The theoretical analysis results show that the fine alignment model is fully self-aligned with no external reference information and the gyrodrifts can be estimated in real time. The simulation results demonstrate that the proposed method can achieve rapid and highly accurate initial alignment for SINS
Characters of homogentisate oxygenase gene mutation and high clonality of the natural pigment-producing Vibrio cholerae strains
<p>Abstract</p> <p>Background</p> <p>Some microorganisms can produce pigments such as melanin, which has been associated with virulence in the host and with a survival advantage in the environment. In <it>Vibrio cholerae</it>, studies have shown that pigment-producing mutants are more virulent than the parental strain in terms of increased UV resistance, production of major virulence factors, and colonization. To date, almost all of the pigmented <it>V. cholerae </it>strains investigated have been induced by chemicals, culture stress, or transposon mutagenesis. However, during our cholera surveillance, some nontoxigenic serogroup O139 strains and one toxigenic O1 strain, which can produce pigment steadily under the commonly used experimental growth conditions, were obtained in different years and from different areas. The genes VC1344 to VC1347, which correspond to the El Tor strain N16961 genome and which comprise an operon in the tyrosine catabolic pathway, have been confirmed to be associated with a pigmented phenotype. In the present study, we investigated the mechanism of pigment production in these strains.</p> <p>Results</p> <p>Sequencing of the VC1344, VC1345, VC1346, and VC1347 genes in these pigmented strains suggested that a deletion mutation in the homogentisate oxygenase gene (VC1345) may be associated with the pigmented phenotype, and gene complementation confirmed the role of this gene in pigment production. An identical 15-bp deletion was found in the VC1345 gene of all six O139 pigment-producing strains examined, and a 10-bp deletion was found in the VC1345 gene of the O1 strain. Strict sequence conservation in the VC1344 gene but higher variance in the other three genes of this operon were observed, indicating the different stress response functions of these genes in environmental adaption and selection. On the basis of pulsed-field gel electrophoresis typing, the pigment-producing O139 strains showed high clonality, even though they were isolated in different years and from different regions. Additionally all these O139 strains belong to the rb4 ribotype, which contains the O139 strains isolated from diarrheal patients, although these strains are cholera toxin negative.</p> <p>Conclusion</p> <p>Dysfunction of homogentisate oxygenase (VC1345) causes homogentisate accumulation and pigment formation in naturally pigmented strains of <it>V. cholerae</it>. The high clonality of these strains may correlate to an environmental survival advantage in the <it>V. cholerae </it>community due to their pigment production, and may imply a potential protective function of melanin in environmental survival of such strains.</p
Study on Deterioration Mechanism and Prevention and Curing Techniques of an RC Bunker
In the coal mining industrial environment, the materials of RC bunkers suffer from serious aging problems. The cracking and deterioration of concrete and the corrosion of steel reinforcement lead to a degeneration of structural performance and a decline of structural reliability. In this paper, based on on-site detection and coal mining ground industrial environment, the deterioration characteristics of RC bunkers were tested. The investigation involved the apparent characteristics, carbonized depth, compression strength of concrete, reinforcement distribution and cover thickness, corrosion rate and mechanical properties of reinforcement, decline degree of bunkers, and so on. Then a design review check was done. Combined with the above information, the cause and mechanisms of cracking and damage of the structure were studied; finally, the problems of RC bunkers were targeted and improved. The work provides a reference method for repairing a deteriorated RC bunker in an aggressive service environment
High-Level PM2.5/PM10 Exposure Is Associated With Alterations in the Human Pharyngeal Microbiota Composition
Previous studies showed that high concentration of particulate matter (PM) 2.5 and PM10 carried a large number of bacterial and archaeal species, including pathogens and opportunistic pathogens. In this study, pharyngeal swabs from 83 subjects working in an open air farmer’s market were sampled before and after exposure to smog with PM2.5 and PM10 levels up to 200 and 300 μg/m3, respectively. Their microbiota were investigated using high-throughput sequencing targeting the V3–V4 regions of the 16S rRNA gene. The genus level phylotypes was increased from 649 to 767 in the post-smog pharyngeal microbiota, of which 142 were new and detected only in the post-smog microbiota. The 142 new genera were traced to sources such as soil, marine, feces, sewage sludge, freshwater, hot springs, and saline lakes. The abundance of the genera Streptococcus, Haemophilus, Moraxella, and Staphylococcus increased in the post-smog pharyngeal microbiota. All six alpha diversity indices and principal component analysis showed that the taxonomic composition of the post-smog pharyngeal microbiota was significantly different to that of the pre-smog pharyngeal microbiota. Redundancy analysis showed that the influences of PM2.5/PM10 exposure and smoking on the taxonomic composition of the pharyngeal microbiota were statistically significant (p < 0.001). Two days of exposure to high concentrations of PM2.5/PM10 changed the pharyngeal microbiota profiles, which may lead to an increase in respiratory diseases. Wearing masks could reduce the effect of high-level PM2.5/PM10 exposure on the pharyngeal microbiota
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