Genome-Wide Association Study and Selective Sweep Analysis Reveal the Genetic Architecture of Body Weights in a Chicken F2 Resource Population

Rapid growth is one of the most important economic traits in broiler breeding programs. Identifying markers and genes for growth traits may not only benefit marker-assisted selection (MAS)/genomic selection (GS) but also provide important information for understanding the genetic architecture of growth traits in broilers. In the present study, an F2 resource population derived from a cross between the broiler and Baier yellow chicken (a Chinese local breed) was used and body weights from 1 to 12 weeks of age [body weight (BW) 1–BW12)] were measured. A total of 519 F2 birds were genome re-sequenced, and a combination of genome-wide association study (GWAS) and selective sweep analysis was carried out to characterize the genetic architecture affecting chicken body weight comprehensively. As a result, 1,539 SNPs with significant effects on body weights at different weeks of age were identified using a genome-wide efficient mixed-model association (GEMMA) package. These SNPs were distributed on chromosomes 1 and 4. Besides, windows under selection identified for BW1–BW12 varied from 1,581 to 2,265. A total of 42 genes were also identified with significant effects on BW1–BW12 based on both GWAS and selective sweep analysis. Among these genes, diacylglycerol kinase eta (DGKH), deleted in lymphocytic leukemia (DLEU7), forkhead box O17 (FOXO1), karyopherin subunit alpha 3 (KPNA3), calcium binding protein 39 like (CAB39L), potassium voltage-gated channel interacting protein 4 (KCNIP4), and slit guidance ligand 2 (SLIT2) were considered as important genes for broiler growth based on their basic functions. The results of this study may supply important information for understanding the genetic architecture of growth traits in broilers

Environmental Impact of Various Rice Cultivation Methods in Northeast China through Life Cycle Assessment

Rice, a crucial staple in China, is cultivated through various techniques, including seedling transplanting, dry direct seeding, and film mulching. Despite its significance, rice production is a considerable environmental burden. Using a life cycle assessment (LCA) methodology, this study aimed to evaluate the environmental impacts of four rice cultivation methods (transplanting rice, dry direct-seeding rice, dry direct-seeding rice with polyethylene film (PE), and dry direct-seeding rice with biodegradable film) in Northeast China. The results indicate that the magnitude of environmental impacts among treatments was consistent across years. The potential values of all environmental impacts of the four different cultivation methods of rice in the 2021 field trial were smaller than the results of the same cultivation method of rice system in the 2022 field trial. Among the four rice cultivation methods, the consumption of energy showed inconsistency over the two years, with the highest energy consumption in the first year being for dry seeding with PE film and in the second year for dry seeding without film. Additionally, transplanting exhibited the highest impact on water resource consumption and climate change. Dry direct-seeding rice displayed the highest eutrophication and ecotoxicity. Dry direct-seeding rice with a biodegradable film had the least impact in terms of acidification. Moreover, dry direct-seeding rice with a biodegradable film minimized water consumption and greenhouse gas emissions without compromising yield

Dry Direct-Seeded Rice Yield and Water Use Efficiency as Affected by Biodegradable Film Mulching in the Northeastern Region of China

In the realm of agriculture, biodegradable films are emerging as a promising substitute for traditional polyethylene (PE) films. Despite their potential, there has been a notable lack of extensive research on their effectiveness in the context of dry direct-seeded rice cultivation. Addressing this gap, a comprehensive biennial study was conducted in the northeastern regions of China, focusing on the ‘Baonong 5’ rice variety. This study meticulously compared three distinct cultivation methods: (1) employing biodegradable film mulching, (2) using conventional plastic film mulching, and (3) cultivating without any mulch. The findings revealed that biodegradable film mulching significantly enhanced soil moisture control, increased leaf area, and improved rice yield and water utilization efficiency (p < 0.05) compared to the plots without mulch. Notably, there was no marked difference in outcomes between the plastic film mulching and the unmulched plots. This research underscores the profound benefits of biodegradable film in rice cultivation, particularly from an environmental sustainability perspective. This innovative method not only boosts agricultural productivity but also addresses critical environmental challenges like climate change and water conservation. The application of biodegradable mulch has proven to be remarkably effective in improving irrigation efficiency and crop water conservation, leading to enhanced rice development and higher yields. The study recorded a substantial increase in water productivity—30% in 2021 and between 52.85% and 60% in 2022—compared to traditional cultivation practices. Furthermore, the use of biodegradable mulch resulted in significantly higher rice yields than the non-mulched plots, thus contributing to increased profitability. Such methods not only yield higher crop outputs but also mitigate environmental issues like water pollution and help alleviate prevalent water shortages in rice farming

Elevated DKK1 expression is an independent unfavorable prognostic indicator of survival in head and neck squamous cell carcinoma

Purpose: DKK1 is an antagonist of the Wnt signaling pathway that has various roles in human physiology. Notably, aberrant DKK1 expression is observed in several cancers. In this retrospective study, we assessed the association between DKK1 expression levels and head and neck squamous cell carcinoma (HNSCC) and its prognostic value. Materials and methods: Using RNA-seq data from HNSCC tumors (N=520) and adjacent normal tissue (N=44) in The Cancer Genome Atlas, we evaluated DKK1 expression levels. Additionally, we evaluated the association of DKK1 expression levels and pathophysiological features of patients with HNSCC and the value of DKK1 expression for prediction of overall survival (OS). We also explored the correlation between DKK1 expression and methylation of its promoter in HNSCC. Results: DKK1 expression was significantly upregulated in HNSCC compared with normal tissues. Moreover, DKK1 expression was significantly associated with smoking, alcohol abuse, sex, human papillomavirus status, tumor site, tumor invasion, and pathologic stage in HNSCC patients. Kaplan-Meier curves showed that high DKK1 expression was correlated with inferior OS. In addition, univariate and multivariate analyses showed that elevated DKK1 expression was an independent prognostic factor for poor OS (HR: 1.85, 95% CI: 1.31-2.62, P<0.001). Regression analysis identified a strong negative correlation between DKK1 expression and methylation of its promoter. Conclusion: These findings support the hypothesis that elevated DKK1 expression is modulated via methylation of its promoter and indicate that DKK1 expression is a highly informative prognostic biomarker for patients with HNSCC

Associations of Transcription Factor 21 Gene Polymorphisms with the Growth and Body Composition Traits in Broilers

This study aims to identify molecular marker loci that could be applied in broiler breeding programs. In this study, we used public databases to locate the Transcription factor 21 (TCF21) gene that affected the economically important traits in broilers. Ten single nucleotide polymorphisms were detected in the TCF21 gene by monoclonal sequencing. The polymorphisms of these 10 SNPs in the TCF21 gene were significantly associated (p &lt; 0.05) with multiple growth and body composition traits. Furthermore, the TT genotype of g.-911T&gt;G was identified to significantly increase the heart weight trait without affecting the negative traits, such as abdominal fat and reproduction by multiple methods. Thus, it was speculated that the g.-911T&gt;G identified in the TCF21 gene might be used in marker-assisted selection in the broiler breeding program

Experimental and Simulation Research on the Preparation of Carbon Nano-Materials by Chemical Vapor Deposition

Carbon nano-materials have been widely used in many fields due to their electron transport, mechanics, and gas adsorption properties. This paper introduces the structure and properties of carbon nano-materials the preparation of carbon nano-materials by chemical vapor deposition method (CVD)&mdash;which is one of the most common preparation methods&mdash;and reaction simulation. A major factor affecting the material structure is its preparation link. Different preparation methods or different conditions will have a great impact on the structure and properties of the material (mechanical properties, electrical properties, magnetism, etc.). The main influencing factors (precursor, substrate, and catalyst) of carbon nano-materials prepared by CVD are summarized. Through simulation, the reaction can be optimized and the growth mode of substances can be controlled. Currently, numerical simulations of the CVD process can be utilized in two ways: changing the CVD reactor structure and observing CVD chemical reactions. Therefore, the development and research status of computational fluid dynamics (CFD) for CVD are summarized, as is the potential of combining experimental studies and numerical simulations to achieve and optimize controllable carbon nano-materials growth

Safety and immunogenicity of a quadrivalent, inactivated, split-virion influenza vaccine (IIV4-W) in healthy people aged 3-60 years: a phase III randomized clinical noninferiority trial

Background A quadrivalent split influenza vaccine IIV4-W against both influenza A and B viruses is urgently needed. Methods To evaluate the safety and immunogenicity of IIV4-W in people aged 3–60 years, 2400 participants recruited in a double-blind phase III trial and were randomly assigned to the IIV4-W, TIV1 and TIV2 groups. The immunogenicity indicators were measured at 28 days postvaccination and for 180 days for safety follow-up. Results Adverse events (AEs) occurred in 162 (20.28%), 116 (14.55%) and 123 (15.41%) participants in the IIV4-W, TIV1 and TIV2 groups, respectively. All these AEs were mild and self-limiting, and no serious AEs related to the vaccines were observed. IIV4-W elicited a non-inferior immune response for matched strains (the lower limit of 95% CI for GMT ratio >0.67, for SCR and SPR difference >-10%) and superior immune response for the additional B strains (the lower limit of 95% CI for GMT ratio >1.5, for SCR difference >10%) versus TIVs. The lower limit of the 95% confidence interval of the GMT increase fold, the seroconversion rate and the seroprotection rate exceeded 2.5, 40% and 70% for the four strains in IIV4-W respectively. Conclusions IIV4-W was noninferior to the TIV-matched strains and was superior to the additional B strain. IIV4-W was safe in the participants and elicited high antibody titers