FEEDBACK CONTROL OF FED-BATCH CULTURES USING RAMAN SPECTROSCOPY FOR METABOLITE ESTIMATION

The monitoring and control of mammalian cell culture processes are critical for the safe and cost-effective development of biotherapeutics in drug development. Current control using off line sampling is infrequent and can miss important metabolic shifts which can impact product quality and consistency. Raman spectroscopy can provide in-line monitoring of multiple metabolites and critical process parameters to allow automation and process improvements. In this research a generic model was developed for monitoring multiple cell lines with a platform Chinese hamster ovary (CHO) cell process. Parameter estimation was then used for the implementation of feedback control. Two nutrient feeds were added based on the culture needs as measured approximately every hour for viable cell and glucose concentrations without operator intervention. This demonstrated comparable, if not improved, control compared to historically adjusting feeds based on daily off-line sampling

Hybrid Motor Drives: Characterization and Control

The purpose of this thesis was to explore the behavior in power sharing and control of Hybrid Motor Drives. In this research, a solar-based hybrid adjustable-speed pump, which has been developed in the laboratory, was used to investigate the power-sharing in hybrid (dual-input) motor-drive systems. The laboratory test setup contained a DC-DC module connected to the DC-bus capacitors of a 2.5hp 230V PWM-based adjustable-speed motor-drive. The experimental results demonstrate that the power-sharing of a Photovoltaic (PV) array/DC-DC converter is not a function of motor speed in hybrid solar-based motor-drives, as long as the power contribution of the AC-grid stays above zero. In these conditions, the PV-DC-DC module behaves like a current source, and the dynamic behavior of the motor is decoupled from the dynamic behavior of the AC-grid, given that the PV-DC-DC module can provide the motor load

Feedback control of intensified fed-batch mammalian cell culture using inline raman spectroscopy

Please click Additional Files below to see the full abstract

A completely automated high inoculation density fed batch process that accommodates clonal diversity and routinely doubles space time yield as compared to low inoculation density processes

Please click Additional Files below to see the full abstract

Slime Bash Social: A Tactile Manipulative for Child and Youth Play

The play product of slime has gained increasing popularity amongst children and youth throughout the United States. Slime can be thought of as a tactile manipulative that can be shaped, stretched, expanded, lumped together, twisted, squeezed, squashed, folded, rolled, shredded, twirled, swirled, pounded, and cut or pulled apart. As a learning tool, slime can and does support STEM initiatives and programs. Slime can be created using simple ingredients that are easily measured, combined, and prepared by children and youth. In addition, slime can incorporate components such as glitter, beads, shaving cream, color dyes and other items. This paper offers an interpretation of slime as a tactile manipulative which can be tied to STEM, art and other initiatives as well as combining with other program areas and formats. The paper also presents program examples drawn from throughout the United States. One program known as the Slime Bash Social sponsored by the University of Northern Iowa’s Institute for Youth Leaders is highlighted in this practical article. This program incorporated slime with other learning activities of dance and singing performances

Controlling pCO2 in high density perfusion cultures

Please click Additional Files below to see the full abstract

Forest degradation drives widespread avian habitat and population declines

In many regions of the world, forest management has reduced old forest and simplified forest structure and composition. We hypothesized that such forest degradation has resulted in long-term habitat loss for forest-associated bird species of eastern Canada (130,017 km2) which, in turn, has caused bird-population declines. Despite little change in overall forest cover, we found substantial reductions in old forest as a result of frequent clear-cutting and a broad-scale transformation to intensified forestry. Back-cast species distribution models revealed that breeding habitat loss occurred for 66% of the 54 most common species from 1985 to 2020 and was strongly associated with reduction in old age classes. Using a long-term, independent dataset, we found that habitat amount predicted population size for 94% of species, and habitat loss was associated with population declines for old-forest species. Forest degradation may therefore be a primary cause of biodiversity decline in managed forest landscapes

The Ursinus Weekly, November 7, 1974

The Great Pumpkin comes to U.C. • Course Directory Catalogue Committee formed by U.S.G.A. • Antigone is ProTheatre\u27s production • Union does it again • Criminal law featured as Forum topic • Letters to the editor: Epitaph on an Ursinus transfer • Pages from Ursinus past: A legend • Perusing Pfahler\u27s pigeons • New Economics head • Equus reviewed • Concerts: Bizarre and sublime • Record review • Nate DuPree: Can a black man find happiness at Ursinus? • Martha Franklin: A half century of service to our students and campus • Readin\u27, ritin\u27 and new math • Introducing campus leaders • Football wrapup: Swarthmore; Widener • Hockey teams win the big games! • Harriers 6-4 on seasonhttps://digitalcommons.ursinus.edu/weekly/1024/thumbnail.jp