VIETNAMESE STUDENT RESEARCHERS’ EXPECTATIONS OF THEIR SUPERVISOR AND SUPERVISION PROCESS

In Vietnam, scientific research is no longer just the work of scientists, graduate students, scholars, or lecturers; tertiary students are also encouraged to conduct scientific research. Therefore, the scientific research work of students receives more attention from educators. The research on carrying out scientific research of students is also therefore increasing. However, researchers do not seem to have paid enough attention to the role of supervisors during the supervision process. The evidence is that there are few studies on supervisors in Vietnam. Therefore, this study was conducted to learn about the role of supervisors from the student's perspective and expectations. Specifically, this study was conducted quantitatively with the use of a questionnaire consisting of 49 questions with a 5-point Likert scale. A total of 100 English-major students at a university in Southwest Vietnam participated in this study by answering the questionnaire. The results from the questionnaire show that students had high expectations from their supervisors. Specifically, students expect their supervisor to be someone who respects their opinions, has good scientific research knowledge, can give constructive comments, and is always willing to help them when needed. Based on research findings, supervisors are encouraged to participate in professional development training related to scientific research to improve their research knowledge and skills. Along with that, supervisors need to be aware of their role during the process of guiding students to do scientific research.  Article visualizations

Pencil lead graphite electrochemically modified with polyglutamic acid as a sensor for detection of enrofloxacin in aqueous media

This study investigates the modification of pencil lead graphite electrodes with polyglutamic acid using an effective and fast static method to develop a sensor for the detection of enrofloxacin (ENR). The successful fabrication of pGA on the electrode surface was confirmed by scanning electron microscopy, energy dispersive X-ray analysis, and Fourier-transform infrared spectroscopy. The conditions of electrochemical modification, including the applied potentials and number of cycles in the potentiostatic process, were systematically investigated to determine their effects on the ENR electrochemical response. The pH of the electrolyte media was also explored to elucidate the electrochemical reaction mechanism of ENR. The developed electrochemical sensor was evaluated using square wave stripping voltammetry for ENR detection. Under optimal conditions, the sensor demonstrated good reproducibility with a relative standard deviation of 4.3% (from five measurements) for ENR signal detection. A linear relationship between ENR concentration and its peak current was observed in the concentration range of 0.1 to 5 µM, with a high correlation coefficient of 0.9988. The limit of detection for ENR using the sensor was 0.12 µM. Our findings provide valuable insights into the design and optimisation of pencil lead graphite electrode-based sensors for ENR detection in aqueous media

Employment, Earnings and Social Protection for Female Workers in Vietnam’s Informal Sector

The informal sector plays an important role in employment creation and poverty reduction among female laborers in Vietnam. Currently, around 70 percent to 80 percent of women in Vietnam work in the informal sector, of which about 60 percent are in the agriculture and 20 percent are in the non-agricultural sectors that are mostly affiliated with small-size production and service units. By investigating the employment, earnings, and social protection policy on female labor in Vietnam’s informal sector, this research is able to answer the following research questions: 1) How important is the role of female labor in the informal sector? 2) What are the important factors that influence job opportunities for women in the informal sector? 3) What are the policy implications on female labor in Vietnam's informal sector? 4) What are the causes of work insecurities among females in Vietnam's informal sector? 5) What areas of social protection are most needed by women workers? 6) How does one address these principal needs for social protection among female workers

Employment, Earnings and Social Protection for Female Workers in Vietnam’s Informal Sector

The informal sector plays an important role in employment creation and poverty reduction among female laborers in Vietnam. Currently, around 70 percent to 80 percent of women in Vietnam work in the informal sector, of which about 60 percent are in the agriculture and 20 percent are in the non-agricultural sectors that are mostly affiliated with small-size production and service units. By investigating the employment, earnings, and social protection policy on female labor in Vietnam’s informal sector, this research is able to answer the following research questions: 1) How important is the role of female labor in the informal sector? 2) What are the important factors that influence job opportunities for women in the informal sector? 3) What are the policy implications on female labor in Vietnam's informal sector? 4) What are the causes of work insecurities among females in Vietnam's informal sector? 5) What areas of social protection are most needed by women workers? 6) How does one address these principal needs for social protection among female workers

Assessment of physical land suitability by GIS-based fuzzy AHP for rubber plantation at the Nam Dong district, Thua Thien Hue province

This research was conducted to determine the main influences and physical factors of land suitability for rubber plantation in the Nam Dong district, Thua Thien Hue province. Six factors such as soil type, soil texture, soil thickness, slope, soil pH and soil organic matter content were considered. Results indicate that soil thickness is has the highest role on the land suitability analysis while soil pH has the lowest. The physical land suitability of rubber plantation was divided into 4 levels: very suitable (10.1%), suitable (15.5%), slightly suitable (3.6%), and currently not suitable (70,8%). This research provides important information for rubber cultivation in projected agricultural land use planning of the Nam Dong district.Nghiên cứu này được thực hiện nhằm xác định sự ảnh hưởng của các yếu tố tự nhiên đến sự thích nghi đất đai của loại hình sử dụng đất trồng cây cao su trên địa bàn huyện Nam Đông, tỉnh Thừa Thiên Huế. Có 6 yếu tố được xem xét trong nghiên cứu này bao gồm: loại đất, thành phần cơ giới, tầng dày canh tác, độ dốc, độ chua và hàm lượng mùn trong đất. Kết quả nghiên cứu chỉ ra rằng tầng dày canh tác là yếu tố có ảnh hưởng lớn nhất đến việc sử dụng đất trồng cây cao su, trong khi đó độ chua là yếu tố có ảnh hưởng ít nhất. Sự thích nghi tự nhiên của loại hình sử dụng đất trồng cây cao su được chia thành 4 mức độ bao gồm rất thích nghi (10,1%), thích nghi (15,5%), tương đối thích nghi (3,6%) và hiện taị không thích nghi (70,8%). Nghiên cứu này cung cấp những thông tin cần thiết và hữu ích cho việc quy hoạch sử dụng đất trồng cây cao su trên địa bàn huyện Nam Đông

The responsibility of C-terminal domain in the thermolabile haemolysin activity of Vibrio parahaemolyticus and inhibition treatments by Phellinus sp. extracts

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 μg of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh

Changing epidemiology and antimicrobial susceptibility of bloodstream infections at a Vietnamese infectious diseases hospital (2010–2020)

Bloodstream infection (BSI) poses a global health problem, with diverse organisms and rising antimicrobial resistance (AMR). Here, we characterized trends in BSI prevalence, AMR, and antibiotic use at a Vietnamese infectious diseases hospital from 2010 to 2020. Among 108,303 cultured blood samples, 8.8% were positive, yielding 7995 pathogens. Of 7553 BSI cases, 86.4% were community-acquired. BSI prevalence varied from 17 to 35 cases/1000 admissions/year, highest in HIV/hepatitis wards and patients >60. The in-hospital mortality or hospice discharge outcome was 21.3%. The top three pathogens, E. coli (24%), K. pneumoniae (8.7%) and S. aureus (8.5%) exhibited increasing prevalence and multidrug resistance. Pathogens like Cryptococcus neoformans (8.4%), Talaromyces marneffei (6.7%), and Salmonella enterica (6.5%) declined. E. coli and K. pneumoniae were prevalent in older adults with community-acquired BSIs. Antibiotic use reached 842.6 DOT/1000 PD and significantly reduced after an antibiotic control policy. Enhanced surveillance and antimicrobial stewardship are crucial for managing BSIs in Vietnam

Outpatient antibiotic prescribing for acute respiratory infections in Vietnamese primary care settings by the WHO AWaRe (Access, Watch and Reserve) classification: An analysis using routinely collected electronic prescription data

Background: This study aims to investigate patterns of antibiotic prescribing and to determine patient-specific factors associated with the choice of antibiotics by the World Health Organization's Access-Watch-Reserve (WHO AWaRe) class for acute respiratory infections (ARIs) in rural primary care settings in northern Vietnam. Methods: We retrospectively reviewed health records for outpatients who were registered with the Vietnamese Health Insurance Scheme, visited one of 112 commune health centres in 6 rural districts of Nam Dinh province, Vietnam during 2019, and were diagnosed with ARIs. Patient-level prescription data were collected from the electronic patient databases. We used descriptive statistics to investigate patterns of antibiotic prescribing, with the primary outcomes including total antibiotic prescriptions and prescriptions by WHO AWaRe group. We identified patient-specific factors associated with watch-group antibiotic prescribing through multivariable logistic regression analysis. Findings: Among 193,010 outpatient visits for ARIs observed in this study, 187,144 (97.0%) resulted in an antibiotic prescription, of which 172,976 (92.5%) were access-antibiotics, 10,765 (5.6%) were watch-antibiotics, 3366 (1.8%) were not-recommended antibiotics. No patients were treated with reserve-antibiotics. The proportion of watch-antibiotic prescription was highest amongst children under 5-years old (18.1%, compared to 9.5% for 5–17-years, 4.9% for 18–49-years, 4.3% for 50–64-years, and 3.7% for 65-and-above-years). In multivariable logistic regression, children, district, ARI-type, comobid chronic respiratory illness, and follow-up visit were associated with higher likelihood of prescribing watch-group antibiotics. Interpretation: The alarmingly high proportion of antibiotic prescriptions for ARIs in primary care, and the frequent use of watch-antibiotics for children, heighten concerns around antibiotic overuse at the community level. Antimicrobial stewardship interventions and policy attention are needed in primary care settings to tackle the growing threat of antibiotic resistance

Implementation of point-of-care testing of C-reactive protein concentrations to improve antibiotic targeting in respiratory illness in Vietnamese primary care: a pragmatic cluster-randomised controlled trial

Background In previous trials, point-of-care testing of C-reactive protein (CRP) concentrations safely reduced antibiotic use in non-severe acute respiratory infections in primary care. However, these trials were done in a research-oriented context with close support from research staff, which could have influenced prescribing practices. To better inform the potential for scaling up point-of-care testing of CRP in respiratory infections, we aimed to do a pragmatic trial of the intervention in a routine care setting. Methods We did a pragmatic, cluster-randomised controlled trial at 48 commune health centres in Viet Nam between June 1, 2020, and May 12, 2021. Eligible centres served populations of more than 3000 people, handled 10–40 respiratory infections per week, had licensed prescribers on site, and maintained electronic patient databases. Centres were randomly allocated (1:1) to provide point-of-care CRP testing plus routine care or routine care only. Randomisation was stratified by district and by baseline prescription level (ie, the proportion of patients with suspected acute respiratory infections to whom antibiotics were prescribed in 2019). Eligible patients were aged 1–65 years and visiting the commune health centre for a suspected acute respiratory infection with at least one focal sign or symptom and symptoms lasting less than 7 days. The primary endpoint was the proportion of patients prescribed an antibiotic at first attendance in the intention-to-treat population. The per-protocol analysis included only people who underwent CRP testing. Secondary safety outcomes included time to resolution of symptoms and frequency of hospitalisation. This trial is registered with ClinicalTrials.gov, NCT03855215. Findings 48 commune health centres were enrolled and randomly assigned, 24 to the intervention group (n=18 621 patients) and 24 to the control group (n=21 235). 17 345 (93·1%) patients in the intervention group were prescribed antibiotics, compared with 20 860 (98·2%) in the control group (adjusted relative risk 0·83 [95% CI 0·66–0·93]). Only 2606 (14%) of 18 621 patients in the intervention group underwent CRP testing and were included in the per-protocol analysis. When analyses were restricted to this population, larger reductions in prescribing were noted in the intervention group compared with the control group (adjusted relative risk 0·64 [95% CI 0·60–0·70]). Time to resolution of symptoms (hazard ratio 0·70 [95% CI 0·39–1·27]) and frequency of hospitalisation (nine in the intervention group vs 17 in the control group; adjusted relative risk 0·52 [95% CI 0·23–1·17]) did not differ between groups. Interpretation Use of point-of-care CRP testing efficaciously reduced prescription of antibiotics in patients with non-severe acute respiratory infections in primary health care in Viet Nam without compromising patient recovery. The low uptake of CRP testing suggests that barriers to implementation and compliance need to be addressed before scale-up of the intervention. Funding Australian Government, UK Government, and the Foundation for Innovative New Diagnostics

A generic assay for whole-genome amplification and deep sequencing of enterovirus A71

Enterovirus A71 (EV-A71) has emerged as the most important cause of large outbreaks of severe and sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infection, and may provide pivotal information for vaccine development. Despite the public health burden of EV-A71, relatively few EV-A71 complete-genome sequences are available for analysis and from limited geographical localities. The availability of an efficient procedure for whole-genome sequencing would stimulate effort to generate more viral sequence data. Herein, we report for the first time the development of a next-generation sequencing based protocol for whole-genome sequencing of EV-A71 directly from clinical specimens. We were able to sequence viruses of subgenogroup C4 and B5, while RNA from culture materials of diverse EV-A71 subgenogroups belonging to both genogroup B and C was successfully amplified. The nature of intra-host genetic diversity was explored in 22 clinical samples, revealing 107 positions carrying minor variants (ranging from 0 to 15 variants per sample). Our analysis of EV-A71 strains sampled in 2013 showed that they all belonged to subgenogroup B5, representing the first report of this subgenogroup in Vietnam. In conclusion, we have successfully developed a high-throughput next-generation sequencing-based assay for whole-genome sequencing of EV-A71 from clinical samples