18 research outputs found
Quantitative nanoscale vortex-imaging using a cryogenic quantum magnetometer
Microscopic studies of superconductors and their vortices play a pivotal role
in our understanding of the mechanisms underlying superconductivity. Local
measurements of penetration depths or magnetic stray-fields enable access to
fundamental aspects of superconductors such as nanoscale variations of
superfluid densities or the symmetry of their order parameter. However,
experimental tools, which offer quantitative, nanoscale magnetometry and
operate over the large range of temperature and magnetic fields relevant to
address many outstanding questions in superconductivity, are still missing.
Here, we demonstrate quantitative, nanoscale magnetic imaging of Pearl vortices
in the cuprate superconductor YBCO, using a scanning quantum sensor in form of
a single Nitrogen-Vacancy (NV) electronic spin in diamond. The sensor-to-sample
distance of ~10nm we achieve allows us to observe striking deviations from the
prevalent monopole approximation in our vortex stray-field images, while we
find excellent quantitative agreement with Pearl's analytic model. Our
experiments yield a non-invasive and unambiguous determination of the system's
local London penetration depth, and are readily extended to higher temperatures
and magnetic fields. These results demonstrate the potential of quantitative
quantum sensors in benchmarking microscopic models of complex electronic
systems and open the door for further exploration of strongly correlated
electron physics using scanning NV magnetometry.Comment: Main text (5 pages, 4 figures) plus supplementary material (5 pages,
6 figures). Comments welcome. Further information under
http://www.quantum-sensing.c
Staphylococcus aureus α-Hemolysin Activates the NLRP3-Inflammasome in Human and Mouse Monocytic Cells
Community Acquired Methicillin Resistant Staphylococcus aureus (CA-MRSA) causes severe necrotizing infections of the skin, soft tissues, and lungs. Staphylococcal α-hemolysin is an essential virulence factor in mouse models of CA-MRSA necrotizing pneumonia. S. aureus α-hemolysin has long been known to induce inflammatory signaling and cell death in host organisms, however the mechanism underlying these signaling events were not well understood. Using highly purified recombinant α-hemolysin, we now demonstrate that α-hemolysin activates the Nucleotide-binding domain and leucine-rich repeat containing gene family, pyrin domain containing 3 protein (NLRP3)-inflammasome, a host inflammatory signaling complex involved in responses to pathogens and endogenous danger signals. Non-cytolytic mutant α-hemolysin molecules fail to elicit NLRP3-inflammasome signaling, demonstrating that the responses are not due to non-specific activation of this innate immune signaling system by bacterially derived proteins. In monocyte-derived cells from humans and mice, inflammasome assembly in response to α-hemolysin results in activation of the cysteine proteinase, caspase-1. We also show that inflammasome activation by α-hemolysin works in conjunction with signaling by other CA-MRSA-derived Pathogen Associated Molecular Patterns (PAMPs) to induce secretion of pro-inflammatory cytokines IL-1β and IL-18. Additionally, α-hemolysin induces cell death in these cells through an NLRP3-dependent program of cellular necrosis, resulting in the release of endogenous pro-inflammatory molecules, like the chromatin-associated protein, High-mobility group box 1 (HMGB1). These studies link the activity of a major S. aureus virulence factor to a specific host signaling pathway. The cellular events linked to inflammasome activity have clear relevance to the disease processes associated with CA-MRSA including tissue necrosis and inflammation
Use of massively parallel molecular dynamics simulations for radiation damage in pyrochlores
DL_POLY_3 is a general purpose molecular dynamics (MD) simulation package designed to simulate systems of the order of tens of millions of particles and beyond by efficiently harnessing the power of modern computer clusters. Here we discuss the package design, functionality and report on performance and capability limits. We then report the application of DL_POLY_3 to study radiation cascades in Gd2Ti2O7 and Gd2Zr2O7, potential materials for high-level radioactive waste storage and discuss problems associated with the analysis of the cascades. We see little direct amorphisation but rather the start of a transition to the fluorite structure which is more pronounced for the Zr than the Ti compound