Mechanical strain induces growth of vascular smooth muscle cells via autocrine action of PDGF.

The effect of cyclic mechanical strain on growth of neonatal rat vascular smooth muscle (VSM) cells were examined. Cells were grown on silicone elastomer plates subjected to cyclic strain (60 cycle/min) by application of a vacuum under the plates. A 48 h exposure to mechanical strain increased the basal rate of thymidine incorporation by threefold and increased cell number by 40% compared with cells grown on stationary rubber plates. Strain also increased the rate of thymidine incorporation in response to alpha-thrombin (from 15- to 33-fold), but not to PDGF. As determined by thymidine autoradiography, strain alone induced a fourfold increase in labeled nuclei at the periphery of dishes, where strain is maximal, and a 2-3-fold increase at the center of dishes. Strain appeared to induce the production of an autocrine growth factor(s), since conditioned medium from cells subjected to strain induced a fourfold increase in DNA synthesis in control cells. Western blots of medium conditioned on the cells subjected to strain indicate that the cells secrete both AA and BB forms of PDGF in response to strain. Northern blots of total cell RNA from cells exposed to strain for 24 h show increased steady-state level of mRNA for PDGF-A. Lastly, polyclonal antibodies to the AA form of PDGF reduced by 75% the mitogenic effect of strain and polyclonal antibodies to AB-PDGF reduced mitogenicity by 50%. Antibodies to bFGF did not significantly reduce the strain-induced thymidine incorporation. Thus, the mechanism of strain-induced growth appears to involve the intermediary action of secreted PDGF

The practice of flexible practice: discussion paper

The term flexible practice describes a way of addressing the needs of GPs to spend time with family or on activities outside their time at work. It has been reported that GPs spend an average of 51.4 hours working per week (CDHFS, 1996) and other data suggest that rural general practitioners work in excess of this. However, it is not just an issue of total working hours. Flexible practice also relates to issues such as quality of life, ability to take leave at short notice, the option to work parttime, ways of dispersing on call duties between different practitioners and health services, easy entry and graceful exit from practices. Other terms used to describe flexible practice arrangements are sustainable practice or sustainable model of practice. Women rural GPs, in particular, have noted that their greatest stress was the conflict between their career and their personal life (Tolhurst et al., 1998, Kilmartin et al., 2002) as they most often carry the main responsibility for the care and rearing of children (Levitt and McEwin, 2001). The three issues contributing to this stress were described as total hours worked, time on call and not finding enough time to keep up their professional knowledge (Tolhurst et al., 1998). Women also commented that the least satisfying part of medical practice was lack of time for family and personal life. Childcare options are often very limited in rural areas, which further contributes to the problem (Tolhurst et al., 1998). In response to these issues, âflexibility was identified as the key to the development and construction of policies and programs to support female GPs in rural and remote practiceâ (Levitt and McEwin, 2001). Lippert (2002) reiterates this from her own research, noting the need for greater flexibility in practice and training arrangements and valuing varied working styles and practice arrangements

The role of electron and phonon temperatures in the helicity-independent all-optical switching of GdFeCo

Ultrafast optical heating of the electrons in ferrimagnetic metals can result in all-optical switching (AOS) of the magnetization. Here we report quantitative measurements of the temperature rise of GdFeCo thin films during helicity-independent AOS. Critical switching fluences are obtained as a function of the initial temperature of the sample and for laser pulse durations from 55 fs to 15 ps. We conclude that non-equilibrium phenomena are necessary for helicity-independent AOS, although the peak electron temperature does not play a critical role. Pump-probe time-resolved experiments show that the switching time increases as the pulse duration increases, with 10 ps pulses resulting in switching times of ~sim 13 ps. These results raise new questions about the fundamental mechanism of helicity-independent AOS.Comment: 18 pages, 6 figures and supplementary material

An Investigation on Cooling of CZT Co-Planar Grid Detectors

The effect of moderate cooling on CdZnTe semiconductor detectors has been studied for the COBRA experiment. Improvements in energy resolution and low energy threshold were observed and quantified as a function of temperature. Leakage currents are found to contribute typically $\sim$5 keV to the widths of photopeaks.Comment: 14 pages, 9 figures. Accepted for publication in Nuclear Inst. and Methods in Physics Research,

Revisiting conditional accounting conservatism in state-controlled firms

This study explores the demand for conditional accounting conservatism from equity shareholders in state-controlled firms. An investigation of state-controlled firms listed on Hong Kong Stock Exchange from 1997 to 2013 shows that the extent of conditional conservatism in state-controlled firms increases when the leverage ratio decreases. It is also found that the high control rights held by the government in state-controlled firms are associated with high conditional conservatism. In addition, further analyses document an offsetting effect between high control rights and firm leverage; a reinforcing effect between high control rights and year of incorporation after 1992; and a substituting effect between high control rights and dividend payments. These findings suggest that the demand from equity shareholders, in addition to the debt demand, can be an important determinant of conditional conservatism and examination of these differing sources of demand can enhance the understanding on accounting conservatism in state-controlled firms

Tracking ALMA System Temperature with Water Vapor Data at High Frequency

The ALMA observatory is now putting more focus on high-frequency observations (frequencies from 275-950 GHz). However, high-frequency observations often suffer from rapid variations in atmospheric opacity that directly affect the system temperature $T_{sys}$. Current observations perform discrete atmospheric calibrations (Atm-cals) every few minutes, with typically 10-20 occurring per hour for high frequency observation and each taking 30-40 seconds. In order to obtain more accurate flux measurements and reduce the number of atmospheric calibrations (Atm-cals), a new method to monitor $T_{sys}$ continuously is proposed using existing data in the measurement set. In this work, we demonstrate the viability of using water vapor radiometer (WVR) data to track the $T_{sys}$ continuously. We find a tight linear correlation between $T_{sys}$ measured using the traditional method and $T_{sys}$ extrapolated based on WVR data with scatter of 0.5-3%. Although the exact form of the linear relation varies among different data sets and spectral windows, we can use a small number of discrete $T_{sys}$ measurements to fit the linear relation and use this heuristic relationship to derive $T_{sys}$ every 10 seconds. Furthermore, we successfully reproduce the observed correlation using atmospheric transmission at microwave (ATM) modeling and demonstrate the viability of a more general method to directly derive the $T_{sys}$ from the modeling. We apply the semi-continuous $T_{sys}$ from heuristic fitting on a few data sets from Band 7 to Band 10 and compare the flux measured using these methods. We find the discrete and continuous $T_{sys}$ methods give us consistent flux measurements with differences up to 5%. Furthermore, this method has significantly reduced the flux uncertainty due to $T_{sys}$ variability for one dataset, which has large precipitable water vapor (PWV) fluctuation, from 10% to 0.7%.Comment: 24 pages, 18 figures, accepted to PAS

Similarity solutions for unsteady shear-stress-driven flow of Newtonian and power-law fluids : slender rivulets and dry patches

Unsteady flow of a thin film of a Newtonian fluid or a non-Newtonian power-law fluid with power-law index N driven by a constant shear stress applied at the free surface, on a plane inclined at an angle α to the horizontal, is considered. Unsteady similarity solutions representing flow of slender rivulets and flow around slender dry patches are obtained. Specifically, solutions are obtained for converging sessile rivulets (0 < α < π/2) and converging dry patches in a pendent film (π/2 < α < π), as well as for diverging pendent rivulets and diverging dry patches in a sessile film. These solutions predict that at any time t, the rivulet and dry patch widen or narrow according to |x|3/2, and the film thickens or thins according to |x|, where x denotes distance down the plane, and that at any station x, the rivulet and dry patch widen or narrow like |t|−1, and the film thickens or thins like |t|−1, independent of N

On the degrees of freedom of lattice electrodynamics

Using Euler's formula for a network of polygons for 2D case (or polyhedra for 3D case), we show that the number of dynamic\textit{\}degrees of freedom of the electric field equals the number of dynamic degrees of freedom of the magnetic field for electrodynamics formulated on a lattice. Instrumental to this identity is the use (at least implicitly) of a dual lattice and of a (spatial) geometric discretization scheme based on discrete differential forms. As a by-product, this analysis also unveils a physical interpretation for Euler's formula and a geometric interpretation for the Hodge decomposition.Comment: 14 pages, 6 figure

High Fill-Out, Extreme Mass Ratio Overcontact Binary Systems. X. The new discovered binary XY Leonis Minoris

The new discovered short-period close binary star, XY LMi, was monitored photometrically since 2006. It is shown that the light curves are typical EW-type and show complete eclipses with an eclipse duration of about 80 minutes. By analyzing the complete B, V, R, and I light curves with the 2003 version of the W-D code, photometric solutions were determined. It is discovered that XY LMi is a high fill-out, extreme mass ratio overcontact binary system with a mass ratio of q=0.148 and a fill-out factor of f=74.1%, suggesting that it is on the late evolutionary stage of late-type tidal-locked binary stars. As observed in other overcontact binary stars, evidence for the presence of two dark spots on both components are given. Based on our 19 epoches of eclipse times, it is found that the orbital period of the overcontact binary is decreasing continuously at a rate of dP/dt=-1.67\times10^{-7}\,days/year, which may be caused by the mass transfer from the primary to the secondary or/and angular momentum loss via magnetic stellar wind. The decrease of the orbital period may result in the increase of the fill-out, and finally, it will evolve into a single rapid-rotation star when the fluid surface reaching the outer critical Roche Lobe.Comment: 19 pages, 4 figures, 9 table