Diagnosis and control of an outbreak of leptospirosis in goats with reproductive failure

Submitted by Repositório Arca ([email protected]) on 2018-07-25T12:28:46Z No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Bio-0014.pdf: 142153 bytes, checksum: 1271076cf3da5ed6df17ef99ccabed66 (MD5)Approved for entry into archive by Priscila Nascimento ([email protected]) on 2018-10-04T20:17:31Z (GMT) No. of bitstreams: 2 Bio-0014.pdf: 142153 bytes, checksum: 1271076cf3da5ed6df17ef99ccabed66 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2018-10-04T20:17:31Z (GMT). No. of bitstreams: 2 Bio-0014.pdf: 142153 bytes, checksum: 1271076cf3da5ed6df17ef99ccabed66 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2012Universidade Federal Fluminense. Laboratory of Veterinary Bacteriology. Niterói, RJ, Brasil. / Universidade Federal Fluminense. Department of Clinical Pathology and Reproduction. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Este estudo apresenta um rebanho de caprinos com falha reprodutiva no período 2009-2010, em que houve abortos (22/50; 44%), reabsorção embrionária (6/50; 12%) e óbitos neonatais (2/50; 4%) . O diagnóstico de leptospirose foi feito com base na sorologia (teste de aglutinação microscópica - MAT), cultura bacteriana e reação em cadeia da polimerase (PCR). Antibioticoterapia, protocolos específicos de vacinação e mudanças nas práticas de manejo foram instigados. Um ano após o surto, os métodos diagnósticos foram repetidos e o desempenho reprodutivo foi re-analisado. Logo após o surto, 61/125 (48,8%) das cabras foram soropositivas para Leptospira. Isolados puros de Leptospira não foram obtidos, mas o teste de PCR Leptospira foi positivo em 48/50 (96%) amostras de urina. Após 1 ano, apenas 4,2% eram soropositivos e a ocorrência de problemas reprodutivos diminuiu cerca de 10 vezes, embora cinco cabras (10,4%) permanecessem PCR-positivas. Uma abordagem de gestão ampla, incluindo métodos de diagnóstico sorológico e molecular, vacinação, tratamento com antibióticos e alteração de alguns aspectos ambientais, foi fundamental para o controle deste surto, minimizando as falhas reprodutivas subsequentes e as perdas econômicas.This study presents a Brazilian goat herd with reproductive failure over 2009–2010, in which there were abortions (22/50; 44%), embryonic resorption (6/50; 12%) and neonatal deaths (2/50; 4%). A diagnosis of leptospirosis was made, based on serology (microscopic agglutination test – MAT), bacterial culture, and polymerase chain reaction (PCR). Antibiotic therapy, specific vaccination protocols and changes in management practices were instigated. One year after the outbreak, diagnostic methods were repeated and reproductive performance re-analysed. Soon after the outbreak, 61/125 (48.8%) of the goats were seropositive for Leptospira. Pure isolates of Leptospira were not obtained, but Leptospira PCR testing was positive in 48/50 (96%) urine samples. After 1 year only 4.2% were seropositive and the occurrence of reproductive problems decreased roughly 10-fold, although five goats (10.4%) remained PCR-positive. A broad-based management approach, including serological and molecular diagnostic methods, vaccination, antibiotic treatment, and alteration of some environmental aspects, were critical to the control of this outbreak, thereby minimising subsequent reproductive failures and economic losses

Molecular analysis of leptospires from serogroup Sejroe obtained from asymptomatic cattle in Rio de Janeiro — Brazil reveals genetic proximity to serovar Guaricura

International audienceBovine leptospirosis causes substantial reproductive failure in cattle, mainly due to infections with serovar (sv) Hardjo infection. Notwithstanding, other serovars from the serogroup (sg) Sejroe could also have important roles in bovine leptospirosis. The objective was to investigate genetic diversity of serogroup Sejroe isolates obtained from asymptomatic cattle in the state of Rio de Janeiro, Brazil. Urine and vaginal fluid (VF) were collected from clinically healthy cattle immediately after slaughter. Five isolates were recovered and characterized (serogrouping) as belonging to sg Sejroe. Sequencing of rrs and secY genes further identified them as Leptospira santarosai. Analysis of secY sequences indicated a high level of sequence homology to sv Guaricura strains. Based on culture and sequence data, we inferred that other members of sg Sejroe may be important in bovine leptospiral infection, particularly genotypes of L. santarosai serovar Guaricura

Molecular Characterization and Serology of Leptospira kirschneri (Serogroup Grippotyphosa) Isolated from Urine of a Mare Post-Abortion in Brazil

Submitted by Repositório Arca ([email protected]) on 2019-03-07T16:36:27Z No. of bitstreams: 7 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) F13-Hamond_et_al-2016-Zoonoses_and_Public_Health.pdf: 187163 bytes, checksum: c311630a753665769ff3a0cb63227d61 (MD5) F134-12917_2014_Article_288.pdf: 1788215 bytes, checksum: 1685ec53b64ec2893274c3feca0ba7cf (MD5) F136-Larentis2012_Article_OptimizationOfMediumFormulatio.pdf: 390655 bytes, checksum: 9f66902ef26e992ec20fbcabbe2737c6 (MD5) F135-pone.0081953.pdf: 1863983 bytes, checksum: 8c2749e2c38abeb0d8c140b59662aa2b (MD5) F131-1-s2.0-S1045105605001065-main.pdf: 156930 bytes, checksum: 35991b0ca5fd175a05cae8a94746f9ee (MD5) F138-1-s2.0-S0264410X10008212-main.pdf: 135707 bytes, checksum: 66e22f02acfb34fb2e8c18433a05941d (MD5)Approved for entry into archive by monique santos ([email protected]) on 2019-04-01T17:39:58Z (GMT) No. of bitstreams: 7 F13-Hamond_et_al-2016-Zoonoses_and_Public_Health.pdf: 187163 bytes, checksum: c311630a753665769ff3a0cb63227d61 (MD5) F134-12917_2014_Article_288.pdf: 1788215 bytes, checksum: 1685ec53b64ec2893274c3feca0ba7cf (MD5) F136-Larentis2012_Article_OptimizationOfMediumFormulatio.pdf: 390655 bytes, checksum: 9f66902ef26e992ec20fbcabbe2737c6 (MD5) F135-pone.0081953.pdf: 1863983 bytes, checksum: 8c2749e2c38abeb0d8c140b59662aa2b (MD5) F131-1-s2.0-S1045105605001065-main.pdf: 156930 bytes, checksum: 35991b0ca5fd175a05cae8a94746f9ee (MD5) F138-1-s2.0-S0264410X10008212-main.pdf: 135707 bytes, checksum: 66e22f02acfb34fb2e8c18433a05941d (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2019-04-01T17:39:58Z (GMT). No. of bitstreams: 7 F13-Hamond_et_al-2016-Zoonoses_and_Public_Health.pdf: 187163 bytes, checksum: c311630a753665769ff3a0cb63227d61 (MD5) F134-12917_2014_Article_288.pdf: 1788215 bytes, checksum: 1685ec53b64ec2893274c3feca0ba7cf (MD5) F136-Larentis2012_Article_OptimizationOfMediumFormulatio.pdf: 390655 bytes, checksum: 9f66902ef26e992ec20fbcabbe2737c6 (MD5) F135-pone.0081953.pdf: 1863983 bytes, checksum: 8c2749e2c38abeb0d8c140b59662aa2b (MD5) F131-1-s2.0-S1045105605001065-main.pdf: 156930 bytes, checksum: 35991b0ca5fd175a05cae8a94746f9ee (MD5) F138-1-s2.0-S0264410X10008212-main.pdf: 135707 bytes, checksum: 66e22f02acfb34fb2e8c18433a05941d (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2016Universidade Federal Fluminense. Department of Microbiology and Parasitology. Laboratory of Veterinary Bacteriology. Niterói, RJ, Brasil.Universidade Federal Fluminense. Department of Microbiology and Parasitology. Laboratory of Veterinary Bacteriology. Niterói, RJ, Brasil.Institut Pasteur. Unite de Biologie des Spiroch etes. National Reference Center. Paris, France.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Unite de Biologie des Spiroch etes. National Reference Center. Paris, France.Universidade Federal Fluminense. Department of Microbiology and Parasitology. Laboratory of Veterinary Bacteriology. Niterói, RJ, Brasil.A strain ofLeptospira kirschneri(serogroup Grippotyphosa) was cultured fromurine of a mare post-abortion in Brazil and characterized by serogrouping, multi-ple-locus variable-number tandem repeat analysis, PGFE, and sequencing ofgenesrrsandsecY. Strains ofL. kirschnerihave apparently never been recoveredfrom horses in tropical area, only in Europe and USA. Knowledge of local epi-demiology is important to interpret genetic profiles of leptospires circulating inan area