Camel Milk and the Prevention of Glucose Cataract, an Organ Culture Study

Purpose: To test if camel milk affects glucose-induced opacity in organ cultured rat and human lenses.Methods: Whole human and rat lenses were cultured in various media containing either 55 mM glucose, camel milk, or a combination of both glucose and milk. Some lenses were cultured in a media containing neither moiety to establish a control. Absorbance spectra of human and rat lenses were measured daily using a visible/ultraviolet light spectrometer. Lens opacities were graded by a blinded grader from photographs taken daily. Aldose reductase activity, catalase activity, glutathione and receptor for advanced glycation end products levels were assayed.Results: The optical density and light scattering intensity of human lenses cultured with glucose were higher after two to four days in organ culture compared with lenses cultured without glucose. Camel milk in the culture media attenuated the glucose-induced increase in optical density, light scattering intensity and opacity grade after two to four days for both human and rat lenses. Aldose reductase activity, catalase activity and glutathione levels were restored but the receptor for advanced glycation end products was similar in rat lenses cultured with glucose compared with those cultured with glucose and camel milk. There were no differences between the assayed moieties in human lenses cultured with glucose or glucose plus milk. Since camel milk restored rat lens glutathione levels, it is possible that camel milk may protect the lens from oxidation and significantly reduce the glucose-induced increase in light scattering of human lenses. Structurally and physiologically, rat lenses are distinct from human lenses, therefore, the rat lens data was highly variable when compared with the human lens data, highlighting the importance of using human lenses in future studies.Conclusions: Camel milk present in the organ culture medium inhibited the glucose-induced opacity in human lenses and restored the amount of glutathione to the same levels of lenses not cultured in glucose. The positive results of the current study leads to future studies to determine the moieties in camel milk that are responsible for cataract inhibition and in vivo studies involving camel milk

Detection of myocardial infarction based on novel deep transfer learning methods for urban healthcare in smart cities

YesOne of the common cardiac disorders is a cardiac attack called Myocardial infarction (MI), which occurs due to the blockage of one or more coronary arteries. Timely treatment of MI is important and slight delay results in severe consequences. Electrocardiogram (ECG) is the main diagnostic tool to monitor and reveal the MI signals. The complex nature of MI signals along with noise poses challenges to doctors for accurate and quick diagnosis. Manually studying large amounts of ECG data can be tedious and time-consuming. Therefore, there is a need for methods to automatically analyze the ECG data and make diagnosis. Number of studies has been presented to address MI detection, but most of these methods are computationally expensive and faces the problem of overfitting while dealing real data. In this paper, an effective computer-aided diagnosis (CAD) system is presented to detect MI signals using the convolution neural network (CNN) for urban healthcare in smart cities. Two types of transfer learning techniques are employed to retrain the pre-trained VGG-Net (Fine-tuning and VGG-Net as fixed feature extractor) and obtained two new networks VGG-MI1 and VGG-MI2. In the VGG-MI1 model, the last layer of the VGG-Net model is replaced with a specific layer according to our requirements and various functions are optimized to reduce overfitting. In the VGG-MI2 model, one layer of the VGG-Net model is selected as a feature descriptor of the ECG images to describe it with informative features. Considering the limited availability of dataset, ECG data is augmented which has increased the classification performance. A standard well-known database Physikalisch-Technische Bundesanstalt (PTB) Diagnostic ECG is used for the validation of the proposed framework. It is evident from experimental results that the proposed framework achieves a high accuracy surpasses the existing methods. In terms of accuracy, sensitivity, and specificity; VGG-MI1 achieved 99.02%, 98.76%, and 99.17%, respectively, while VGG-MI2 models achieved an accuracy of 99.22%, a sensitivity of 99.15%, and a specificity of 99.49%.This project was funded by University of Jeddah, Jeddah, Saudi Arabia (Project number: UJ-02-018-ICGR)

Whole-genome sequencing reveals host factors underlying critical COVID-19

Critical COVID-19 is caused by immune-mediated inflammatory lung injury. Host genetic variation influences the development of illness requiring critical care1 or hospitalization2,3,4 after infection with SARS-CoV-2. The GenOMICC (Genetics of Mortality in Critical Care) study enables the comparison of genomes from individuals who are critically ill with those of population controls to find underlying disease mechanisms. Here we use whole-genome sequencing in 7,491 critically ill individuals compared with 48,400 controls to discover and replicate 23 independent variants that significantly predispose to critical COVID-19. We identify 16 new independent associations, including variants within genes that are involved in interferon signalling (IL10RB and PLSCR1), leucocyte differentiation (BCL11A) and blood-type antigen secretor status (FUT2). Using transcriptome-wide association and colocalization to infer the effect of gene expression on disease severity, we find evidence that implicates multiple genes—including reduced expression of a membrane flippase (ATP11A), and increased expression of a mucin (MUC1)—in critical disease. Mendelian randomization provides evidence in support of causal roles for myeloid cell adhesion molecules (SELE, ICAM5 and CD209) and the coagulation factor F8, all of which are potentially druggable targets. Our results are broadly consistent with a multi-component model of COVID-19 pathophysiology, in which at least two distinct mechanisms can predispose to life-threatening disease: failure to control viral replication; or an enhanced tendency towards pulmonary inflammation and intravascular coagulation. We show that comparison between cases of critical illness and population controls is highly efficient for the detection of therapeutically relevant mechanisms of disease

Effect of periodontal treatment on serum C-reactive protein level in obese and normal-weight women affected with chronic periodontitis

Contains fulltext : 111056.pdf (publisher's version ) (Open Access)OBJECTIVE: To investigate the effect of conventional periodontal therapy on serum C-reactive protein (CRP) level and periodontal status in obese and normal-weight chronic periodontitis patients. METHODS: This is a controlled clinical trial conducted at the King Abdulaziz University Faculty of Dentistry, Jeddah, Kingdom of Saudi Arabia between December 2009 and March 2011. A total of 40 women affected with moderate to severe chronic periodontitis were selected (20 obese [test group] and 20 normal-weight [control]). Smokers, pregnant women, and subjects with any systemic disease were excluded. Serum CRP level and periodontal parameters, including clinical attachment level, probing depth, bleeding on probing and plaque scores were assessed at baseline, and 2 months after non-surgical periodontal treatment. RESULTS: Periodontal therapy was effective in reducing gingival inflammation, as well as serum CRP level in the total sample and within each group. The pre-treatment mean level of serum CRP was 0.78 (+/-0.51) and post- treatment was 0.55 (+/-0.41) mg/l in the total sample (p=0.001). A tendency was observed toward a better systemic response to treatment in normal-weight compared to obese women, however, it was not statistically significant (the mean changes in CRP levels after therapy were 0.28 [+/-0.43] and 0.19 [+/-0.32] mg/l). CONCLUSION: Periodontal treatment is effective in reducing systemic inflammation as measured by serum CRP level, and obesity does not have a major negative impact on response to periodontal therapy

Bone regeneration related to calcium phosphate-coated implants in osteoporotic animal models: a meta-analysis

Contains fulltext : 110417.pdf (publisher's version ) (Closed access)BACKGROUND: Osteoporosis is a frequent human metabolic bone disorder. Prospectively, global ageing of populations will lead to a major increase of subjects being diagnosed with osteoporosis and in need for dental rehabilitation. However, as local osteoporosis of the jaws affects bone quantity and quality of edentulous regions, osseointegration of dental implants might be hampered. Consequently, calcium phosphate ceramic-coated implants have been suggested to compensate for low bone quantity/density and for impaired bone healing in osteoporosis. Nonetheless, up to now no meta-analytical assessment of the relevant preclinical literature to quantify such a possible positive effect has been undertaken. MATERIALS AND METHODS: PubMed search, limited to animal models, to identify a possible positive effect of calcium phosphate-coated implants on bone regeneration, was carried out. Further, the reference lists of related review articles and publications selected for inclusion in this review were systematically screened. The primary outcome variables were bone-to-implant contact percentage as assessed histomorphometrically and mechanical stability testing. RESULTS: The electronic search in the database of the National Library of Medicine resulted in the identification of 2704 titles. These titles were initially screened by the two independent reviewers for possible inclusion, resulting in further consideration of 51 publications. Screening the abstracts led to 22 full-text articles. From these articles, 16 reports were excluded. Finally, six of these original research reports could be selected for evaluation. Additionally, eight publications were identified by manual search. Thus, a total of 14 articles were included for analysis. CONCLUSIONS: It was concluded that (1) in osteoporotic animal models calcium phosphate ceramic-coated implants are associated with improved bone-to-implant healing as compared to noncoated implants. Moreover, (2) essentially due to quality characteristics of the analyzed original research articles a negative impact of osteoporosis on bone-to-implant healing could not be confirmed. Besides, (3) the established positive bone-to-implant healing effect of calcium phosphate ceramic coatings does not differ between osteoporotic and nonosteoporotic, healthy animal models

Attitude of medical students at King Saud Bin Abdulaziz University for Health Sciences toward family medicine as a future specialty.

Contains fulltext : 215388.pdf (publisher's version ) (Open Access)BACKGROUND: Owing to the rising population of the Kingdom of Saudi Arabia, the need for family physicians is growing. The number of family physicians who would be available in the health service in future is dependent on the attitudes of medical students because their choice of specialty is a major factor in satisfying this demand. The aim of the study was to evaluate the attitudes of medical students to family medicine as a future career. MATERIALS AND METHODS: This cross-sectional study was conducted at King Saud Bin Abdulaziz University for Health Sciences. A total of 308 students were randomly selected from problem-based learning groups. Data were collected using a 25-item validated questionnaire, and Excel and SPSS were used for data entry and analysis. Mean and standard deviation were used to describe numerical data and frequencies and percentages to describe categorical data. P < 0.05 was considered statistically significant. RESULTS: A total of 308 medical students, 201 (65.3%) of whom were male, completed the questionnaire. Majority of the students (229 [74.3%]) agreed that family physicians make important contributions to medicine although family medicine was one of the least preferred specialties of the students. CONCLUSIONS: Most students were aware of the importance of family medicine; however, only a few of them chose this specialty. Further studies should be conducted to identify the factors that influence medical students' decisions in their choice of family medicine as a future career

Visualization of calcium phosphate cement in teeth by zero echo time H-1 MRI at high field

Contains fulltext : 184112.pdf (publisher's version ) (Closed access

Osteogenesis around CaP-coated titanium implants visualized using 3D histology and micro-computed tomography

Item does not contain fulltextCalcium phosphate (CaP) coatings can enhance the performance of bone implants in compromised conditions, such as osteoporosis. Therefore, this study compared non-coated vs. CaP-coated (n = 8) titanium implants in osteoporotic ovariectomized (OVX) rats. Bone volume (BV) was assessed using micro-computer tomography (micro-CT) and three-dimensional (3D) histology, in three zones from the implant surface. Bone remodeling was assessed using fluorochrome labels and osteoclast staining. Micro-CT and 3D histology showed a BV reduction in OVX animals, of respectively 22.4 and 10.5%. BV was significantly increased inside all zones around CaP coatings, especially in the inner zone of the OVX animals. Fluorochrome labels were predominantly seen when the coating was applied. Osteoclasts were mainly found in the area remote from the surface of non-coated implants in control animals. For the coated implants, osteoclasts were distributed evenly, and present in direct vicinity of the surface. In conclusion, 3D histology is a suitable technique to obtain data and insight into bone architecture around implants at relatively high resolution. Bone formation was significantly reduced in osteoporotic animals. CaP coatings resulted in a higher BV directly around implants installed in osteoporotic animals, enhanced turnover, and a shift of remodeling activity toward the implant surface. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3463-3473, 2015

Evaluation of Peri-Implant Bone Grafting Around Surface-Porous Dental Implants: An In Vivo Study in a Goat Model

Contains fulltext : 214148.pdf (publisher's version ) (Open Access

Biological Effect of Single or Combined Pharmacological Therapy Using Alendronate and Simvastatin on Implant Osseointegration: An In Vivo Study in Healthy and Osteoporotic Rat Models

Contains fulltext : 221436.pdf (publisher's version ) (Open Access