29,120 research outputs found
Heat sink capability of jet A fuel - Heat transfer and coking studies
Heat sink capabilities of Jet-A fuel - heat transfer and coking studie
Indicators of replicative damage in equine tendon fibroblast monolayers
<p>Background:
Superficial digital flexor tendon (SDFT) injuries of horses usually follow cumulative matrix microdamage; it is not known why the reparative abilities of tendon fibroblasts are overwhelmed or subverted. Relevant in vitro studies of this process require fibroblasts not already responding to stresses caused by the cell culture protocols. We investigated indicators of replicative damage in SDFT fibroblast monolayers, effects of this on their reparative ability, and measures that can be taken to reduce it.</p>
<p>Results:
We found significant evidence of replicative stress, initially observing consistently large numbers of binucleate (BN) cells. A more variable but prominent feature was the presence of numerous gammaH2AX (γH2AX) puncta in nuclei, this being a histone protein that is phosphorylated in response to DNA double-stranded breaks (DSBs). Enrichment for injury detection and cell cycle arrest factors (p53 (ser15) and p21) occurred most frequently in BN cells; however, their numbers did not correlate with DNA damage levels and it is likely that the two processes have different causative mechanisms. Such remarkable levels of injury and binucleation are usually associated with irradiation, or treatment with cytoskeletal-disrupting agents.</p>
<p>Both DSBs and BN cells were greatest in subconfluent (replicating) monolayers. The DNA-damaged cells co-expressed the replication markers TPX2/repp86 and centromere protein F. Once damaged in the early stages of culture establishment, fibroblasts continued to express DNA breaks with each replicative cycle. However, significant levels of cell death were not measured, suggesting that DNA repair was occurring. Comet assays showed that DNA repair was delayed in proportion to levels of genotoxic stress.</p>
<p>Conclusions:
Researchers using tendon fibroblast monolayers should assess their “health” using γH2AX labelling. Continued use of early passage cultures expressing initially high levels of γH2AX puncta should be avoided for mechanistic studies and ex-vivo therapeutic applications, as this will not be resolved with further replicative cycling. Low density cell culture should be avoided as it enriches for both DNA damage and mitotic defects (polyploidy). As monolayers differing only slightly in baseline DNA damage levels showed markedly variable responses to a further injury, studies of effects of various stressors on tendon cells must be very carefully controlled.</p>
A research program to reduce interior noise in general aviation airplanes. Design of an acoustic panel test facility
The design, construction, and costs of a test facility for determining the sound transmission loss characteristics of various panels and panel treatments are described. The pressurization system and electronic equipment used in experimental testing are discussed as well as the reliability of the facility and the data gathered. Tests results are compared to pertinent acoustical theories for panel behavior and minor anomalies in the data are examined. A method for predicting panel behavior in the stiffness region is also presented
Experimental Study of the Role of Atomic Interactions on Quantum Transport
We report an experimental study of quantum transport for atoms confined in a
periodic potential and compare between thermal and BEC initial conditions. We
observe ballistic transport for all values of well depth and initial
conditions, and the measured expansion velocity for thermal atoms is in
excellent agreement with a single-particle model. For weak wells, the expansion
of the BEC is also in excellent agreement with single-particle theory, using an
effective temperature. We observe a crossover to a new regime for the BEC case
as the well depth is increased, indicating the importance of interactions on
quantum transport.Comment: 4 pages, 3 figure
Role of Activity in Human Dynamics
The human society is a very complex system; still, there are several
non-trivial, general features. One type of them is the presence of power-law
distributed quantities in temporal statistics. In this Letter, we focus on the
origin of power-laws in rating of movies. We present a systematic empirical
exploration of the time between two consecutive ratings of movies (the
interevent time). At an aggregate level, we find a monotonous relation between
the activity of individuals and the power-law exponent of the interevent-time
distribution. At an individual level, we observe a heavy-tailed distribution
for each user, as well as a negative correlation between the activity and the
width of the distribution. We support these findings by a similar data set from
mobile phone text-message communication. Our results demonstrate a significant
role of the activity of individuals on the society-level patterns of human
behavior. We believe this is a common character in the interest-driven human
dynamics, corresponding to (but different from) the universality classes of
task-driven dynamics.Comment: 5 pages, 6 figures. Accepted by EP
Invariant quantum discord in qubit-qutrit systems under local dephasing
We investigate the dynamics of quantum discord and entanglement for a
class of mixed qubit-qutrit states assuming that only the qutrit is under the action of a dephasing channel. We demonstrate that even though the entanglement in the qubit-qutrit state disappears in a finite time interval, partial coherence left in the system enables quantum discord to remain invariant throughout the whole time evolution
High-resolution imaging of ultracold fermions in microscopically tailored optical potentials
We report on the local probing and preparation of an ultracold Fermi gas on
the length scale of one micrometer, i.e. of the order of the Fermi wavelength.
The essential tool of our experimental setup is a pair of identical,
high-resolution microscope objectives. One of the microscope objectives allows
local imaging of the trapped Fermi gas of 6Li atoms with a maximum resolution
of 660 nm, while the other enables the generation of arbitrary optical dipole
potentials on the same length scale. Employing a 2D acousto-optical deflector,
we demonstrate the formation of several trapping geometries including a tightly
focussed single optical dipole trap, a 4x4-site two-dimensional optical lattice
and a 8-site ring lattice configuration. Furthermore, we show the ability to
load and detect a small number of atoms in these trapping potentials. A site
separation of down to one micrometer in combination with the low mass of 6Li
results in tunneling rates which are sufficiently large for the implementation
of Hubbard-models with the designed geometries.Comment: 15 pages, 6 figure
Stimulated Raman scattering in an optical parametric oscillator based on periodically poled MgO-doped stoichiometric LiTaO3
The evolution versus pump power of the spectrum of a singly resonant optical
parametric oscillator based on an MgO-doped periodically poled stoichiometric
lithium tantalate crystal is observed. The onset of cascade Raman lasing due to
stimulated Raman scattering in the nonlinear crystal is analyzed. Spurious
frequency doubling and sum-frequency generation phenomena are observed and
understood. A strong reduction of the intracavity Raman scattering is obtained
by a careful adjustment of the cavity losses.Comment: 6 figure
Preparing the foundations for video-based, practice-placement support: establishing the role from a students’ perspective
Currently, many placement-based health programme students within the UK are supported through face-to-face visits from university staff. Whilst cited in literature as being of value, the face-to-face nature of this contact is not supported. Alternatives including video-based communications methods offer the potential for cost effective, environmentally responsible support. However, in order to establish the fitness for purpose of alternative approaches, the content and purpose of current support needs to be understood. This project aimed to investigate student perceptions of the ideal content and purpose of clinical support visits, and alternatives to the current face-to-face approach. Fifty-six Physiotherapy undergraduate students responded to questionnaires with a further nine participating in a follow-up focus group. Participants emphasised the value of the visit in guiding learning, ensuring progression and resolving arising issues, and highlighted concerns over alternative approaches. Focus group participants discussed the importance of personal and professional confidence in directing requirements for support, and went on to propose a menu of options for methods of communication.Whilst limited in some applications, video technologies may be one of the options. Overall, however, this project supports the need for consideration of individualised learning journeys within curriculum planning
- …