Применение буровых шламов в изготовлении строительных материалов

Thrombomodulin is a membrane-bound protein that plays an active role in the blood coagulation system by binding thrombin and initiating the protein C anticoagulant pathway. Solulin (TM) is a recombinant soluble derivative of human thrombomodulin. It is used for the treatment of thrombotic disorders. To evaluate the production of this pharmaceutical protein in plants, expression vectors were generated using four different N-terminal signal peptides. Immunoblot analysis of transiently transformed tobacco leaves showed that intact Solulin (TM) could be detected using three of these signal peptides. Furthermore transgenic tobacco plants and BY2 cells producing Solulin (TM) were generated. Immunoblot experiments showed that Solulin (TM) accumulated to maximum levels of 115 and 27 mu g g(-1)supercript stop plant material in tobacco plants and BY2 cells, respectively. Activity tests performed on the culture supernatant of transformed BY2 cells showed that the secreted Solulin (TM) was functional. In contrast, thrombomodulin activity was not detected in total soluble protein extracts from BY2 cells, probably due to inhibitory effects of substances in the cell extract. N-terminal sequencing was carried out on partially purified Solulin (TM) from the BY2 culture supernatant. The sequence was identical to that of Solulin (TM) produced in Chinese hamster ovary cells, confirming correct processing of the N-terminal signal peptide. We have demonstrated that plants and plant cell cultures can be used as alternative systems for the production of an active recombinant thrombomodulin derivative

Applications of Two-Body Dirac Equations to the Meson Spectrum with Three versus Two Covariant Interactions, SU(3) Mixing, and Comparison to a Quasipotential Approach

In a previous paper Crater and Van Alstine applied the Two Body Dirac equations of constraint dynamics to the meson quark-antiquark bound states using a relativistic extention of the Adler-Piran potential and compared their spectral results to those from other approaches, ones which also considered meson spectroscopy as a whole and not in parts. In this paper we explore in more detail the differences and similarities in an important subset of those approaches, the quasipotential approach. In the earlier paper, the transformation properties of the quark-antiquark potentials were limited to a scalar and an electromagnetic-like four vector, with the former accounting for the confining aspects of the overall potential, and the latter the short range portion. A part of that work consisted of developing a way in which the static Adler-Piran potential was apportioned between those two different types of potentials in addition to covariantization. Here we make a change in this apportionment that leads to a substantial improvement in the resultant spectroscopy by including a time-like confining vector potential over and above the scalar confining one and the electromagnetic-like vector potential. Our fit includes 19 more mesons than the earlier results and we modify the scalar portion of the potential in such a way that allows this formalism to account for the isoscalar mesons {\eta} and {\eta}' not included in the previous work. Continuing the comparisons made in the previous paper with other approaches to meson spectroscopy we examine in this paper the quasipotential approach of Ebert, Faustov, and Galkin for a comparison with our formalism and spectral results.Comment: Revisions of earlier versio

Short and long term treatment of asthma with intravenous nutrients

BACKGROUND: Asthma is an increasing problem in this country and others. Although medications for the treatment of asthma abound and are improving, there are inherent risks and side effects with all of them. Intravenous magnesium has been employed in the treatment of acute asthma, but its use has not become universal, nor has it been studied for the treatment of chronic asthma. It is known to be a safe drug with minimal side effects. In this study, the author investigates the use of magnesium and other nutrients in the treatment of both acute and chronic asthma. METHODS: In this non-blinded outcome study, following informed consent, forty-three (43) randomly selected volunteer patients with both acute and chronic asthma were treated with IV infusions described herein. All patients were observed with spirometry 10 minutes post-infusion; two sub-groups of patients were also observed after multiple infusions over a short period of time (less than one month) and a longer period of time (average 5.8 months). Pulmonary function was analyzed by spirometric testing with pre- and post-infusion spirometric measurements with the pre/post group. For longer term (Trend) patients, baseline spirometry measurements were compared to spirometry measurements after patients had received multiple infusions over a period of time. Eight (8) patients were measured for both pre/post and Trend data. RESULTS: The 38 pre-infusion/post-infusion patients with acute and chronic asthma demonstrated an overall average improvement (percentage improvement in percent predicted) of 45%. The 13 patients measured for improvement over time (Trend data, average duration 5.82 months), demonstrated an overall average improvement (percentage improvement in percent predicted) of 57%. Of the 13 patients in the multiple infusion group, 9 patients who received longer-term therapy (average duration of 12.58 months) for chronic asthma demonstrated an overall average improvement of 95% (percentage improvement in percent predicted). CONCLUSION: The use of intravenous treatment with multiple nutrients, including magnesium, for acute and chronic asthma may be of considerable benefit. Pulmonary function improved progressively the longer patients received treatment

Urbane Resilienz gegenüber extremen Wetterereignissen: Gemeinsamer Verbundabschlussbericht des Forschungsprojektes ExTrass

Das Projekt ExTrass hatte zwei Ziele: Das erste Ziel war es, Klimaresilienz in den drei Fallstudienstädten Potsdam, Remscheid und Würzburg messbar zu stärken. Das zweite Ziel war es, Transferpotenziale zwischen Groß- und Mittelstädten in Deutschland zu identifizieren und besser nutzbar zu machen, sodass die Wirkung von Pilotvorhaben über die direkt involvierten Städte hinausgehen kann. Dies sollte in enger Zusammenarbeit mit den Stadtverwaltungen sowie zivilgesellschaftlichen Akteur:innen des Katastrophenschutzes erfolgen. Dabei standen folgende Leitfragen im Fokus: • Wie verbreitet sind Klimaanpassungsaktivitäten in Großstädten und größeren kreisfreien Mittelstädten in Deutschland? • Welche hemmenden und begünstigenden Faktoren beeinflussen die Klimaanpassung? • Welche Maßnahmen der Klimaanpassung werden tatsächlich umgesetzt, und wie kann die Umsetzung verbessert werden? Was behindert? • Inwiefern lassen sich Beispiele guter Praxis auf andere Städte übertragen, adaptieren oder weiterentwickeln

Unifying Results On Hamiltonian Claw-Free Graphs

This work was motivated by many (recent) papers on hamiltonicity of claw-free graphs, i.e. graphs that do not contain K 1;3 as an induced subgraph. By combining ideas from these papers with some new observations, we unify several of the existing sufficiency results, using a new sufficient condition consisting of seven subconditions. If each pair of vertices at distance two of a 2-connected claw-free graph G satisfies at least one of these subconditions, then G is hamiltonian. We also present infinite classes of examples of graphs showing that these subconditions are, in some sense, independent. AMS Subject Classifications (1991): 05 C 45 Key words: claw-free graph, hamiltonian graph, degree condition, local connectivity, forbidden subgraph Part of the work was done while subsets of the authors met at Aachen and Plzen. The work of the second and third author was supported by EC-grant No. 927. 1 1. TERMINOLOGY AND NOTATION We use [1] for terminology and notation not defined here and co..