Algunas transferrinas no comunes en bovinos

En un "muestreo" de 3.000 bovinos de distintas razas y mestizos provenientes de la Estación Experimental Agropecuaria de Balcarce (I.N.T.A.), (años 1970-1971), fueron detectados 10 fenotipos de Transferrinas "no-comunes" en esta especie animal. Uno de los fenotipos aparece con dos bandas proteicas lentas D 2 de las cuatro normales, constituyendo un tipo anormal de acuerdo con SPOONER Y RAXTER (1969. Estos autores refieren la acción de un gene epistático recesivo que afecta al ácido siálico vinculado a las dos bandas más veloces. Otros fenotipos se expresan con bandas agregadas y desplazamientos electroforéticos sobre gel de almidón hidrolizado, no coincidentes con los fenotipos conocidos. Frente a la inusitada rareza de los fenotipos observados, se decidió confirmar el hallazgo por marcación con Fe 59, demostrándose la veracidad del descubrimiento en vacunos de nuestro país.In a sample of 3.000 bovines of different breeds and half-breeds from the Estación Experimental Agropecuaria de Balcarce, I.N T A., (years 1970-1971), were detected 10 "not-common" Transferrin phenotypes in this animal species. One of the D 2 phenotypes appears with only two élow protein bands and not the four normal bands, constituting an anormal type according with SPOONER AND BAXTER (1969). These authors refer the recessive epistatic gene action affecting the sialic acid binding to the two faster protein bands. Other phenotypes are expressed with aggregated bands and electrophoretic displacement on hydrolyzed starch gel not-coincident with the acquainted phenotypes. Because of the unusual rarity of the observed phenotypes, it was decided to confirm the finding with Fe 59 added to every serum demonstrating the veracity of the discovery in bovines of our country.Facultad de Ciencias Veterinaria

Analysis of mendelian segregation of erythrocyte phenogroups in criollo cattle by the sire family method

Como continuación de las investigaciones realizadas sobre Inmunogenética en Bovinos Criollos de Argentina, presentamos algunos resultados de segregación de "Marcadores 'rimunogenéticos" o Fenogrupos Sanguíneos paternos por el Método "Toro-Familia" (Stormont et al., 1951; Miller, 1960) que promueven pautas de interés para el reconocimiento e identificación genética de esta singular raza bovina. La segregación y frecuencia de cada fenogrupo, especialmente del Sistema B, inducen a clarificar la Herencia Mendeliana Grupal en estudios raciales específicos. Se analizan 10 locus génicos en correspondencia a 10 Sistemas de Grupos Sanguíneos Eritrocitarios de esta especie animal.As a continuation of the immunogenetic research on Criollo cattle of Argentina, we present segregation results of several Immunogenetic markers or phenogroups of the sire's blood by the Sire Family method. This model method serves as a guide to characterize and genetically identify this unique bovine type. The segregation and frequency of each phenogroup, especially of the B system, clarifies its Mendelian heredity and the comparative position of Criollo cattle in specific breed studies. Ten genetic loci are analyzed corresponding to ten erythro. In the Chapters II and III planification basis are considerated, named objectifies, showing the operative magnitude of the Programmed for different areas of the country, with especial emphasis in the animal dynamics population the programmed actions for the control and eradication of the disease for areas in differents places: rural, urban and suburban. Moreover, a theoric study about the necessary budget and ways to implement it, it's done in basis to global estimation in the diferent areas of the country. In the Chapter IV, themes about Sanitary Education, according to operative level, are elaborated; with according of the proposal objectifies, for the regional and chronological programation. In the Chapter V it is prossed the necessity of legal and reglamentary reformes of the actual system. And the Chapter VI, give sorne indispensables techniques bases for control and evaluation of the elaborated National Plan.Facultad de Ciencias Veterinaria

Algunas transferrinas no comunes en bovinos

En un "muestreo" de 3.000 bovinos de distintas razas y mestizos provenientes de la Estación Experimental Agropecuaria de Balcarce (I.N.T.A.), (años 1970-1971), fueron detectados 10 fenotipos de Transferrinas "no-comunes" en esta especie animal. Uno de los fenotipos aparece con dos bandas proteicas lentas D 2 de las cuatro normales, constituyendo un tipo anormal de acuerdo con SPOONER Y RAXTER (1969. Estos autores refieren la acción de un gene epistático recesivo que afecta al ácido siálico vinculado a las dos bandas más veloces. Otros fenotipos se expresan con bandas agregadas y desplazamientos electroforéticos sobre gel de almidón hidrolizado, no coincidentes con los fenotipos conocidos. Frente a la inusitada rareza de los fenotipos observados, se decidió confirmar el hallazgo por marcación con Fe 59, demostrándose la veracidad del descubrimiento en vacunos de nuestro país.In a sample of 3.000 bovines of different breeds and half-breeds from the Estación Experimental Agropecuaria de Balcarce, I.N T A., (years 1970-1971), were detected 10 "not-common" Transferrin phenotypes in this animal species. One of the D 2 phenotypes appears with only two élow protein bands and not the four normal bands, constituting an anormal type according with SPOONER AND BAXTER (1969). These authors refer the recessive epistatic gene action affecting the sialic acid binding to the two faster protein bands. Other phenotypes are expressed with aggregated bands and electrophoretic displacement on hydrolyzed starch gel not-coincident with the acquainted phenotypes. Because of the unusual rarity of the observed phenotypes, it was decided to confirm the finding with Fe 59 added to every serum demonstrating the veracity of the discovery in bovines of our country.Facultad de Ciencias Veterinaria

Variación del color de macroalga Lessonia trabeculata deshidratada con secador de rayos infrarrojos

Fishmeal concentrates a high percentage of protein and its monetary cost is relatively high, searching for possible alternatives in macroalgae for improvement purposes is an option. Color is a fundamental characteristic of food, however, drying by the Infrared method as an emerging technology for the study of the macroalga Lessonia trabeculata is little studied. For this reason, the degradation and change of color in the drying process by infrared rays (IR) at different temperatures 40°C, 55°C and 70°C were objectively characterized with the color space scale (CIE-L*a *b*), through the use of a spectrophotometer. Drying was carried out with infrared rays in an infrared radiant heating chamber with a power of 4.5kW, 20.5 Amps. The results indicate that the best color treatment determined was for the macroalga Lessonia trabeculata at a temperature of 40°C with values ​​for: L*= 44.758±0.227, a*= -1.564 ± 0.016 and b*= 11.050±0.017; obtaining a first order kinetics for logarithmic scale corresponding to the parameters L*.b* as a function of time, likewise an activation energy value of Ea = 24.062 KJ/mol and the Arrhenius constant k0 = 0.0197 min- was reached. one. It is concluded that there is inverse variability between the color of the macroalgae and the temperature applied in the drying process; being the values ​​of the color parameters L*, a*, b* of the macroalga decreases as the temperature increases, thereby leading to color degradation.La harina de pescado concentra alto porcentaje de proteínas y su costo monetario es relativamente alto, buscar posibles alternativas en las macroalgas con propósitos de mejora es una opción. El color es una característica primordial de los alimentos, sin embargo, el secado por el método Infrarrojo como tecnología emergente para el estudio de la macroalga Lessonia trabeculata es poco estudiado. Por ello, se caracterizó objetivamente la degradación y cambio del color en el proceso de secado por rayos infrarrojos (IR) a diferentes temperaturas 40°C, 55°C y 70°C con la escala de espacio de color (CIE-L*a*b*), a través del uso de un espectrofotómetro. Se procedió a secar con rayos infrarrojos en una cámara de calentamiento radiante infrarrojo de potencia 4.5kW, 20.5 Amperios. Los resultados indican que el mejor tratamiento de color determinado fue para la macroalga Lessonia trabeculata a una temperatura de 40°C con valores para: L*= 44,758±0,227, a*= -1.564 ± 0,016 y b* = 11,050±0,017; obteniendo una cinética de primer orden para escala logarítmica correspondiente a los parámetros L*.b* en función al tiempo, asimismo se alcanzó un valor de Energía de activación de Ea = 24.062 KJ/mol y de la constante de Arrhenius k0 = 0.0197 min-1.  Se concluye que existe variabilidad inversa entre el color de la macroalga y la temperatura aplicada en el proceso de secado; siendo los valores de los parámetros de color L*, a*, b* de la macroalga disminuye a medida que se incrementa la temperatura, acarreando con ello la degradación del color

Strong Directional Out-of-Plane Scattering in Multiple Ionizing Highly Charged Ion-Atom Collisions

The azimuthal ({phi}{sub r}) and polar angle ({theta}{sub r}) scattering of projectiles in coincidence with recoil ions has been studied for 0.53 MeV/u F{sup 8+} + Ne. For high degree of ionization of the target we find the resultant transverse momentum of all electrons emitted into the continuum to increase with the number of ejected electrons and to have a direction mostly not co-planar with the scattering plane

Analysis of mendelian segregation of erythrocyte phenogroups in criollo cattle by the sire family method

Como continuación de las investigaciones realizadas sobre Inmunogenética en Bovinos Criollos de Argentina, presentamos algunos resultados de segregación de "Marcadores 'rimunogenéticos" o Fenogrupos Sanguíneos paternos por el Método "Toro-Familia" (Stormont et al., 1951; Miller, 1960) que promueven pautas de interés para el reconocimiento e identificación genética de esta singular raza bovina. La segregación y frecuencia de cada fenogrupo, especialmente del Sistema B, inducen a clarificar la Herencia Mendeliana Grupal en estudios raciales específicos. Se analizan 10 locus génicos en correspondencia a 10 Sistemas de Grupos Sanguíneos Eritrocitarios de esta especie animal.As a continuation of the immunogenetic research on Criollo cattle of Argentina, we present segregation results of several Immunogenetic markers or phenogroups of the sire's blood by the Sire Family method. This model method serves as a guide to characterize and genetically identify this unique bovine type. The segregation and frequency of each phenogroup, especially of the B system, clarifies its Mendelian heredity and the comparative position of Criollo cattle in specific breed studies. Ten genetic loci are analyzed corresponding to ten erythro. In the Chapters II and III planification basis are considerated, named objectifies, showing the operative magnitude of the Programmed for different areas of the country, with especial emphasis in the animal dynamics population the programmed actions for the control and eradication of the disease for areas in differents places: rural, urban and suburban. Moreover, a theoric study about the necessary budget and ways to implement it, it's done in basis to global estimation in the diferent areas of the country. In the Chapter IV, themes about Sanitary Education, according to operative level, are elaborated; with according of the proposal objectifies, for the regional and chronological programation. In the Chapter V it is prossed the necessity of legal and reglamentary reformes of the actual system. And the Chapter VI, give sorne indispensables techniques bases for control and evaluation of the elaborated National Plan.Facultad de Ciencias Veterinaria

Disentangling signatures of selection before and after European colonization in latin Americans

Throughout human evolutionary history, large-scale migrations have led to intermixing (i.e., admixture) between previously separated human groups. Although classical and recent work have shown that studying admixture can yield novel historical insights, the extent to which this process contributed to adaptation remains underexplored. Here, we introduce a novel statistical model, specific to admixed populations, that identifies loci under selection while determining whether the selection likely occurred post-admixture or prior to admixture in one of the ancestral source populations. Through extensive simulations, we show that this method is able to detect selection, even in recently formed admixed populations, and to accurately differentiate between selection occurring in the ancestral or admixed population. We apply this method to genome-wide SNP data of ∼4,000 individuals in five admixed Latin American cohorts from Brazil, Chile, Colombia, Mexico, and Peru. Our approach replicates previous reports of selection in the human leukocyte antigen region that are consistent with selection post-admixture. We also report novel signals of selection in genomic regions spanning 47 genes, reinforcing many of these signals with an alternative, commonly used local-ancestry-inference approach. These signals include several genes involved in immunity, which may reflect responses to endemic pathogens of the Americas and to the challenge of infectious disease brought by European contact. In addition, some of the strongest signals inferred to be under selection in the Native American ancestral groups of modern Latin Americans overlap with genes implicated in energy metabolism phenotypes, plausibly reflecting adaptations to novel dietary sources available in the Americas

Nitric Oxide-Sensitive Guanylyl Cyclase Is Differentially Regulated by Nuclear and Non-Nuclear Estrogen Pathways in Anterior Pituitary Gland

17β-estradiol (E2) regulates hormonal release as well as proliferation and cell death in the pituitary. The main nitric oxide receptor, nitric oxide sensitive- or soluble guanylyl cyclase (sGC), is a heterodimer composed of two subunits, α and β, that catalyses cGMP formation. α1β1 is the most abundant and widely expressed heterodimer, showing the greater activity. Previously we have shown that E2 decreased sGC activity but exerts opposite effects on sGC subunits increasing α1 and decreasing β1 mRNA and protein levels. In the present work we investigate the mechanisms by which E2 differentially regulates sGC subunits' expression on rat anterior pituitary gland. Experiments were performed on primary cultures of anterior pituitary cells from adult female Wistar rats at random stages of estrous cycle. After 6 h of E2 treatment, α1 mRNA and protein expression is increased while β1 levels are down-regulated. E2 effects on sGC expression are partially dependent on de novo transcription while de novo translation is fully required. E2 treatment decreased HuR mRNA stabilization factor and increased AUF1 p37 mRNA destabilization factor. E2-elicited β1 mRNA decrease correlates with a mRNA destabilization environment in the anterior pituitary gland. On the other hand, after 6 h of treatment, E2-BSA (1 nM) and E2-dendrimer conjugate (EDC, 1 nM) were unable to modify α1 or β1 mRNA levels, showing that nuclear receptor is involved in E2 actions. However, at earlier times (3 h), 1 nM EDC causes a transient decrease of α1 in a PI3k-dependent fashion. Our results show for the first time that E2 is able to exert opposite actions in the anterior pituitary gland, depending on the activation of classical or non-classical pathways. Thus, E2 can also modify sGC expression through membrane-initiated signals bringing to light a new point of regulation in NO/sGC pathway