19,210 research outputs found

    Thinking out of the box?What teachers must do to foster higher order thinking skills in accounting graduates.

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    This paper addresses the concern that accounting education is inadequate in equipping accounting graduates for the challenges of today' s workforce. The study posits that students need to be taught higher-order thinking skills and to engage in deep learning in order to succeed in today's workplace. The underlying belief is deep learning is hard work and unless students are taught critical thinking skills, they cannot initiate deep learning, let alone sustain the effort

    A system to enrich for primitive streak-derivatives, definitive endoderm and mesoderm, from pluripotent cells in culture

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    Two lineages of endoderm develop during mammalian embryogenesis, the primitive endoderm in the pre-implantation blastocyst and the definitive endoderm at gastrulation. This complexity of endoderm cell populations is mirrored during pluripotent cell differentiation in vitro and has hindered the identification and purification of the definitive endoderm for use as a substrate for further differentiation. The aggregation and differentiation of early primitive ectoderm-like (EPL) cells, resulting in the formation of EPL-cell derived embryoid bodies (EPLEBs), is a model of gastrulation that progresses through the sequential formation of primitive streak-like intermediates to nascent mesoderm and more differentiated mesoderm populations. EPL cell-derived EBs have been further analysed for the formation of definitive endoderm by detailed morphological studies, gene expression and a protein uptake assay. In comparison to embryoid bodies derived from ES cells, which form primitive and definitive endoderm, the endoderm compartment of embryoid bodies formed from EPL cells was comprised almost exclusively of definitive endoderm. Definitive endoderm was defined as a population of squamous cells that expressed Sox17, CXCR4 and Trh, which formed without the prior formation of primitive endoderm and was unable to endocytose horseradish peroxidase from the medium. Definitive endoderm formed in EPLEBs provides a substrate for further differentiation into specific endoderm lineages; these lineages can be used as research tools for understanding the mechanisms controlling lineage establishment and the nature of the transient intermediates formed. The similarity between mouse EPL cells and human ES cells suggests EPLEBs can be used as a model system for the development of technologies to enrich for the formation of human ES cell-derived definitive endoderm in the future.Sveltana Vassilieva, Hweee Ngee Goh, Kevin X. Lau, James N. Hughes, Mary Familari, Peter D. Rathjen and Joy Rathje

    Eficácia do ivermectin no controle do piolho (Haematopinus tuberculatus) em búfalos.

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    Perfil hemático de bezerros búfalos lactentes naturalmente parasitados pelo Neoascaris vitulorum.

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    bitstream/item/31934/1/CPATU-BP69.pd

    Electron interferometry with nano-gratings

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    We present an electron interferometer based on near-field diffraction from two nanostructure gratings. Lau fringes are observed with an imaging detector, and revivals in the fringe visibility occur as the separation between gratings is increased from 0 to 3 mm. This verifies that electron beams diffracted by nanostructures remain coherent after propagating farther than the Talbot length zT=2d2/λz_T = 2d^2/\lambda = 1.2 mm, and hence is a proof of principle for the function of a Talbot-Lau interferometer for electrons. Distorted fringes due to a phase object demonstrates an application for this new type of electron interferometer.Comment: 4 pgs, 6 figure
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