Demonstrations of maternal effects of Atlantic cod: combining the use of unique mesocosm and novel molecular techniques - a new EU-project

One of the effects of fishing is a reduction in average age and size in exploited fish stocks, leading to an increasing proportion of recruit spawners in the stock. Current management practice assumes equal viability of offspring from first time spawners and from repeat spawners, despite the fact that that first time spawners often produce smaller eggs than older spawners. The aim of this EU-project is to follow offspring from families of first time spawning and older cod, reared under identical and semi-natural conditions in marine enclosures (mesocosms). The parental origin of the larvae is identified using microsatellite DNA methodology. The advantage of this approach, compared to traditional laboratory rearing, is that rearing conditions are close to natural conditions, and all larvae are reared in the same environment. This eliminates the tank-to-tank variability often observed in traditional rearing experiments. The fish are reared from hatching, through the larval and juvenile stages, until sexual maturity. Growth rates, survival and nutritional condition will be measured using methods such as RNA/DNA ratio and otolith micro increment analysis. The results will be related to parental origin and quality measures of the eggs. It is intended to incorporate the results into management models for improvement of fishery management strategies. In this paper we will focus on a description of the projec

Culture optimization for the emergent zooplanktonic model organism Oikopleura dioica

The pan-global marine appendicularian, Oikopleura dioica, shows considerable promise as a candidate model organism for cross-disciplinary research ranging from chordate genetics and evolution to molecular ecology research. This urochordate, has a simplified anatomical organization, remains transparent throughout an exceptionally short life cycle of less than 1 week and exhibits high fecundity. At 70 Mb, the compact, sequenced genome ranks among the smallest known metazoan genomes, with both gene regulatory and intronic regions highly reduced in size. The organism occupies an important trophic role in marine ecosystems and is a significant contributor to global vertical carbon flux. Among the short list of bona fide biological model organisms, all share the property that they are amenable to long-term maintenance in laboratory cultures. Here, we tested diet regimes, spawn densities and dilutions and seawater treatment, leading to optimization of a detailed culture protocol that permits sustainable long-term maintenance of O. dioica, allowing continuous, uninterrupted production of source material for experimentation. The culture protocol can be quickly adapted in both coastal and inland laboratories and should promote rapid development of the many original research perspectives the animal offers

Can limited ocean mixing buffer rapid climate change?

It has been argued that diapycnal mixing has a strongly stabilizing role in the global thermohaline circulation (THC). Negative feedback between THC transport and low-latitude buoyancy distribution is present in theory based on thermocline scaling, but is absent from Stommel's classical model. Here, it is demonstrated that these two models can be viewed as opposite limits of a single theory. Stommel's model represents unlimited diapycnal mixing, whereas the thermocline scaling represents weak mixing. The latter limit is more applicable to the modern ocean, and previous studies suggest that it is associated with a more stable THC. A new box model, which can operate near either limit, is developed to enable explicit analysis of the transient behaviour. The model is perturbed from equilibrium with an increase in surface freshwater forcing, and initially behaves as if the only feedbacks are those present in Stommel's model. The response is buffered by any upper ocean horizontal mixing, then by propagation of salinity anomalies, each of which are stabilizing mechanisms. However, negative feedback associated with limited diapycnal mixing only prevents thermohaline catastrophe in a modest parameter domain. This is because the time-scale associated with vertical advective-diffusive balance is much longer than the time required for the THC to change mode. The model is then tuned to allow equilibrium THC transport to be independent of the rate of mixing. The equilibrium surface salinity difference controls the classical THC-transport/salinity positive feedback, whereas the equilibrium interior density difference controls the mean-flow negative feedback. When mixing is strong, unrealistic vertical homogenization occurs, causing a convergence in surface and interior meridional gradients. This reduces positive feedback, and increases stability, in the tuned model. Therefore, Stommel's model appears to overestimate, rather than underestimate, THC stability to high-frequency changes in forcing.<br/