Plasma lipids and lipoproteins in the grass-cutter, thryonomys swznderianus, in captivity'

The grass-cutter, Thryonomys sinderiaruls (TEMMINCK 1827), an african hystricomorph rodent, represents a popular meat, much sought after in Africa south of the Sahara. Unfortunately, breeding of this animal is far from being acceptable in terms of effíiency, probably because of the insufficient infarmation available on its biology and physiology. Lipid and lipoprotein profiles were done on the plasma or serum of 49 male grass-cutters. We showed that: 1. The concentrations of lipids and lipoproteins were a quarter to half the values found in man; 2. The serum lipoproteins could be separated into 4 distinct fractions by discontinuous polyacrylamide gel electrophoresis; 3. The grass-cutter could be classified as HDL mammal; 4. Human and grass-cutter apolipoproteins A-IV, B, C-III, and E carry partial immunological crossr eactivity . Further characterization of the grass-cutter lipoproteins is needed as is the investigation of the role of diet composition on these profilesLa rata de cañaveral, Thryonomys swinderianui (TEMMINCK 1827), roedor histricomorfo africano representa una popular fuente de alimentación, muy apreciada en África al sur del Sahara Desgraciadamente, la eficacia de la crianza de este animal está lejos de ser aceptada, probablemente debido a que no se tiene suficiente información sobre su biología y su fisiología. En este estudio, se ha realizado un perfil lipídico y lipoproteico plasmático sobre 49 ratas de cañaveral machos. Los resultados muestran que: 1. Las concentraciones en lípidos y lipoproteínas de la rata de cañaveral se sitúan entre 114 a 112 de los valores encontrados en el hombre. 2. Las lipoproteínas del animal pueden separarse en 4 fracciones distintas por electroforesis sobre gel de poliacrilamida en gradiente discontinuo. 3. La rata & cañaveral puede ser considerado como un «mamífero de HDL». 4. Las apolipoproteínas A-IV, B, C-III y E del hombre y de la rata de cañaveral presentan una identidad antig6nica común. Un análisis más detallado de las lipoproteínas de este animal deber6 ser realizado, así como la investigación de la influencia que tiene el régimen alimentario en estos perfiles

Mallotojaponins B and C: Total Synthesis, Antiparasitic Evaluation, and Preliminary SAR Studies

The first total syntheses of mallotojaponin B and C as well as several analogues have been achieved. Biological evaluation of the synthesized compounds against <i>Plasmodium falciparum</i> and <i>Trypanosoma brucei</i> have also been carried out

Detection, characterization, and screening of heme-binding molecules by mass spectrometry for malaria drug discovery

Contains fulltext : 110844.pdf (publisher's version ) (Open Access)Drug screening for antimalarials uses heme biocrystallization inhibition methods as an alternative to parasite cultures, but they involve complex processes and cannot detect artemisinin-like molecules. The described method detects heme-binding compounds by mass spectrometry, using dissociation of the drug-heme adducts to evaluate putative antiplasmodial activity. Applied to a chemical library, it showed a good hit-to-lead ratio and is an efficient early stage screening for complex mixtures like natural extracts

2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations

International audienceNonsense mutations cause about 10% of genetic disease cases, and no treatments are available. Nonsense mutations can be corrected by molecules with nonsense mutation readthrough activity. An extract of the mushroom Lepista inversa has recently shown high-efficiency correction of UGA and UAA nonsense mutations. One active constituent of this extract is 2,6-diaminopurine (DAP). In Calu-6 cancer cells, in which TP53 gene has a UGA nonsense mutation, DAP treatment increases p53 level. It also decreases the growth of tumors arising from Calu-6 cells injected into immunodeficient nude mice. DAP acts by interfering with the activity of a tRNA-specific 2'-O-methyltransferase (FTSJ1) responsible for cytosine 34 modification in tRNATrp. Low-toxicity and high-efficiency UGA nonsense mutation correction make DAP a good candidate for the development of treatments for genetic diseases caused by nonsense mutations