789 research outputs found
Superadiabatic dynamical density functional study of Brownian hard-spheres in time-dependent external potentials
Superadiabatic dynamical density functional theory (superadiabatic-DDFT), a
first-principles approach based on the inhomogeneous two-body correlation
functions, is employed to investigate the response of interacting Brownian
particles to time-dependent external driving. Predictions for the
superadiabatic dynamics of the one-body density are made directly from the
underlying interparticle interactions, without need for either adjustable fit
parameters or simulation input. The external potentials we investigate have
been chosen to probe distinct aspects of structural relaxation in dense,
strongly interacting liquid states. Nonequilibrium density profiles predicted
by the superadiabatic theory are compared with those obtained from both
adiabatic DDFT and event-driven Brownian dynamics simulation. Our findings show
that superadiabatic-DDFT accurately predicts the time-evolution of the one-body
density
Mean-Field Theory of Inhomogeneous Fluids
The Barker-Henderson perturbation theory is a bedrock of liquid-state
physics, providing quantitative predictions for the bulk thermodynamic
properties of realistic model systems. However, this successful method has not
been exploited for the study of inhomogeneous systems. We develop and implement
a first-principles 'Barker-Henderson density functional', thus providing a
robust and quantitatively accurate theory for classical fluids in external
fields. Numerical results are presented for the hard-core Yukawa model in three
dimensions. Our predictions for the density around a fixed test particle and
between planar walls are in very good agreement with simulation data. The
density profiles for the free liquid vapour interface show the expected
oscillatory decay into the bulk liquid as the temperature is reduced towards
the triple point, but with an amplitude much smaller than that predicted by the
standard mean-field density functional
Quantifying the Energetics and Length Scales of Carbon Segregation to Fe Symmetric Tilt Grain Boundaries Using Atomistic Simulations
Segregation of impurities to grain boundaries plays an important role in both
the stability and macroscopic behavior of polycrystalline materials. The
research objective in this work is to better characterize the energetics and
length scales involved with the process of solute and impurity segregation to
grain boundaries. Molecular dynamics simulations are used to calculate the
segregation energies for carbon within multiple grain boundary sites over a
database of 125 symmetric tilt grain boundaries in Fe. The simulation results
show that the majority of atomic sites near the grain boundary have segregation
energies lower than in the bulk. Moreover, depending on the boundary, the
segregation energies approach the bulk value approximately 5-12 \AA\ away from
the center of the grain boundary, providing an energetic length scale for
carbon segregation. A subsequent data reduction and statistical representation
of this dataset provides critical information such as about the mean
segregation energy and the associated energy distributions for carbon atoms as
a function of distance from the grain boundary, which quantitatively informs
higher scale models with energetics and length scales necessary for capturing
the segregation behavior of impurities in Fe. The significance of this research
is the development of a methodology capable of ascertaining segregation
energies over a wide range of grain boundary character (typical of that
observed in polycrystalline materials), which herein has been applied to carbon
segregation in a specific class of grain boundaries in iron
Risk factors for brucellosis and knowledge-attitude practice among pastoralists in Afar and Somali regions of Ethiopia
BACKGROUND: Brucellosis is a neglected bacterial zoonotic disease with substantial economic impact on households. Pastoral communities are a potential risk group due to their way of life being closely interlinked with their large livestock herds. METHODOLOGY: A semi-structured questionnaire survey was conducted in households in the pastoral Afar and Somali (SRS) regions. All households had people and animals serologically tested for brucellosis. Questions were related to husbandry, consumption habits, and knowledge-attitude-practice towards the disease and zoonoses. Descriptive statistics and logistic analysis were performed to assess potential risk factors for having households with positive humans and/or animals. RESULT: 647 households were included in the survey. Herd brucellosis prevalence was 40.3 % (15.9-86.3 % in Afar; 4-72.2 % in SRS). Over half (56.3 %) of the households in Afar and 41.8 % in SRS had at least one human reactor. Nearly a quarter of the households (22.8 %), recalled abortions in goats in the last 12 months, whereas 52.5 % and 50.3 % recalled stillborn in all species and membrane retentions respectively. All respondents drank raw milk and discarded animal afterbirths in the direct surroundings with minimal protection. Risk factors for animal reactors were goat herd size, and goat abortion. There was no identified risk factor for having human reactors in households. None of the households knew about brucellosis. CONCLUSION: Although being endemic in Afar and SRS, Brucellosis is not known by the pastoralists. Brucellosis control programs will have to be tailored to the pastoral context, accounting for their mobility, large, multi-species herds and habits
Molecular epidemiology of tuberculosis in the Somali region, eastern Ethiopia
Background: Tuberculosis (TB) is one of the leading causes of morbidity and mortality in low-income countries like Ethiopia. However, because of the limited laboratory infrastructure there is a shortage of comprehensive data on the genotypes of clinical isolates of Mycobacterium tuberculosis (M. tuberculosis) complex (MTBC) in peripheral regions of Ethiopia. The objective of this study was to characterize MTBC isolates in the Somali region of eastern Ethiopia. Methods: A cross-sectional study was conducted in three health institutions between October 2018 and December 2019 in the capital of Somali region. A total of 323 MTBC isolates (249 from pulmonary TB and 74 from extrapulmonary TB) were analyzed using regions of difference 9 (RD 9)-based polymerase chain reaction (PCR) and spoligotyping. Results: Of the 323 MTBC isolates, 99.7% (95% CI: 99.1-100%) were M. tuberculosis while the remaining one isolate was M. bovis based on RD 9-based PCR. Spoligotyping identified 71 spoligotype patterns; 61 shared types and 10 orphans. A majority of the isolates were grouped in shared types while the remaining grouped in orphans. The M. tuberculosis lineages identified in this study were lineage 1, 2, 3, 4, and 7 with the percentages of 7.4, 2.2, 28.2, 60.4, and 0.6%, respectively. Most (87.9%) of the isolates were classified in clustered spoligotypes while the remaining 12.1% isolates were singletons. The predominant clustered spoligotypes identified were SIT 149, SIT 21, SIT 26, SIT 53, and SIT 52, each consisting of 17.6, 13.3, 8.4, 7.4, and 5%, respectively. Lineage 3 and lineage 4, as well as the age group (15-24), were associated significantly with clustering. Conclusion: The MTBC isolated from TB patients in Somali region were highly diverse, with considerable spoligotype clustering which suggests active TB transmission. In addition, the Beijing spoligotype was isolated in relatively higher frequency than the frequencies of its isolation from the other regions of Ethiopia warranting the attention of the TB Control Program of the Somali region
Integrated human-animal sero-surveillance of Brucellosis in the pastoral Afar and Somali regions of Ethiopia
BACKGROUND: Brucellosis is widespread in Ethiopia with variable reported prevalence depending on the geographical area, husbandry practices and animal species. However, there is limited information on the disease prevalence amongst pastoral communities, whose life is intricately linked with their livestock. METHODOLOGY: We conducted an integrated human-animal brucellosis sero-surveillance study in two adjacent pastoral regions, Afar and Somali region (SRS). This cross-sectional study included 13 woredas (districts) and 650 households. Blood samples were collected from people and livestock species (cattle, camel, goats and sheep). Sera were analyzed with C-ELISA for camels and shoats (sheep and goats), with I-ELISA for cattle and IgG ELISA for humans. Descriptive and inferential statistics analyses were performed. RESULTS: A total of 5469 sera were tested by ELISA. Prevalence of livestock was 9.0% in Afar and 8.6% in SRS (ranging from 0.6 to 20.2% at woreda level). In humans, prevalence was 48.3% in Afar and 34.9% in SRS (ranging from 0.0 to 74.5% at woreda level). 68.4% of all households in Afar and 57.5% of households in SRS had at least one animal reactor. Overall, 4.1% of animals had a history of abortion. The proportion of animals with abortion history was higher in seropositive animals than in seronegative animals. Risk factor analysis showed that female animals were significantly at higher risk of being reactors (p = 0.013). Among the species, cattle had the least risk of being reactors (p = 0.014). In humans, there was a clear regional association of disease prevalence (p = 0.002). The older the people, the highest the odds of being seropositive. CONCLUSION: Brucellosis is widespread in humans and animals in pastoral communities of Afar and SRS with the existence of geographical hotspots. No clear association was seen between human and particular livestock species prevalence, hence there was no indication as whether B. abortus or B. melitensis are circulating in these areas, which warrants further molecular research prior to embarking on a national control programs. Such programs will need to be tailored to the pastoral context
The CD95 Receptor: Apoptosis Revisited
CD95 is the quintessential death receptor and, when it is bound by ligand, cells undergo apoptosis. Recent evidence suggests, however, that CD95 mediates not only apoptosis but also diverse nonapoptotic functions depending on the tissue and the conditions
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