Histological analysis of motoneuron survival and microglia inhibition after nerve root avulsion treated with nerve graft implantation and minocycline: an experimental study

Motor vehicle accidents are the most common cause of injuries involving avulsion of the brachial plexus in humans, resulting in debilitating motor dysfunction. Lack of an established animal model to test drug treatments hinders the introduction of new pharmacological agents. Avulsion injury of cervical ventral roots can be replicated in rats, resulting in a progressive loss of the motoneurons and increase in neurotoxic expression of microglia. This is a report on the effect of prompt nerve implantation and minocycline treatment on the suppression of microglia activation and survival of motoneurons. 20 adult female Sprague-Dawley rats were used for this study, which was approved by the Animal Ethical Committee, USM (approval number /2011/(73)(346)). The animals underwent surgical avulsion of the C6 nerve root, followed by reimplantation with peripheral nerve graft and treatment with intraperitoneal minocycline. At 6 weeks postoperatively, immunohistochemistry using primary antibody Iba1 (microglia) and nicotinamide adenine dinucleotide phosphate diaphorase (NADPh) with neutral-red staining (motoneuron) under flourescence microscopy was performed at the C6 spinal cord segment and then quantified. This study showed signifi cant reduction of microglia expression in the study group; mean ranks of control and study group were 15.2 and 11.6, respectively; U=9.5, Z=3.02, p0.05. This may be due to the effect of the surgery; the surgery has the potential to cause additional trauma to the cord parenchyma, leading to further motoneuron loss and an increase in scarring around the avulsed region, thus impeding regeneration of the motoneuron

Optical characterization on ITO/TiO2/P3HT/Areca Catechu/au for thin film hybrid solar cell

In this research, dye sensitized solar cell is fabricated by a combination of inorganic titanium dioxide nanoparticles sensitized by a locally available natural dye extract from organic Areca Catechu nut. This hybrid solar cells are fabricated accordingly by deposition of ITO/TiO2/P3HT/Areca Catechu/Au by using electrochemical method. The deposition rates of TiO2 are varied from 0.05, 0.07, 0.09 to 0.11 vs-1 whereas the number of scan of each layers are fixed to 5. The absorption spectra analysis is carried out in the wavelength range 200 to 600 nm, showed a wide and significant absorption spectrum in UV and visible regions. Analysis shows that scan rate affects the electrical conductivity of hybrid solar cell. The highest conductivity is recorded at 0.278 Scm-1 corresponding to the scan rate of 0.07 Vs-1at a potential value of 3.5 V

Understanding media literacy in relation to adolescent soft drink consumption behavior

This paper examines relationship between media literacy and the Theory of Planned Behavior (TPB) variables focusing on consumption of soft drink among adolescents in Klang Valley, Malaysia. In addition, this paper also determines soft drink consumption, level of media literacy and the influences of the TPB variables on the intention of soft drink consumption. A cluster sampling method was used in collecting data within the Klang Valley area. The sample consisted of 436 adolescents from secondary school, aged between 13 to 18 years old. Two main scales utilized were Media Literacy (ML) scale and TPB scale. The descriptive, multiple linear regression and Pearson product-moment correlation analyses were carried out to answer the research objectives. Results revealed that 36% of respondents drank a minimum of 1 can, bottle or glass of soft drink for the past seven days and possess good level of media literacy (35%).Meanwhile, 14% of variance in adolescents’ intention of soft drink consumption is explained by TPB variables. Additionally, the total media literacy score towards soft drink advertisement was significantly positively correlated with attitude (r = 0.250, p<.05) and subjective norms (r = 0.174, p<.05). Conversely, total media literacy was significant negatively correlated with intention to consume soft drink (r = -0.19, p<.05) and indicated that media literacy could lower the intention to consume soft drink, thus can reduce soft drink consumption among adolescents. The importance of this study is to help the authorities, soft drink marketers and producers in Malaysia to better understand media literacy and adolescent soft drink consumption behavior

Kenaf Fiber Composite in Automotive Industry: An Overview

Recently, natural fibers become an attractive to automotive industry as an alternative reinforcement for glass fiber reinforced thermoplastics. Additionally, natural fiber components in the automotive industry can provide numerous advantages compared to synthetic conventional such as reduction of weight and cost,  recyclability, renewability and in addition to eco-efficiency. Thus, the use of natural fibers in automotive industry has shown increasingly stringent environmental criteria. Furthermore, amongst grouped bast fibers such as flax, hemp, jute, ramie and kenaf; kenaf fiber seen as potential natural fiber with robust mechanical properties. Kenaf fiber had been explored to enhance desired mechanical properties as an automotive structural components. As usual, natural fibres have some issues and disadvantages when used as reinforcements for polymeric composites. Therefore, some modification performed on fibers such as chemical treatment was carried out. In addition, the use of a coupling agent and a plasticizer can also increase fiber-matrix adhesive bonding

Near infrared spectroscopy (NIRS) applications in medical: non-invasive and invasive leukemia screening

Near Infrared Spectroscopy (NIRS) has been applied as analytical tool in numerous field of study due to its ability in non-invasive application. NIRS with the ability in providing the information on biological molecules shows a high potential as a diagnosis tool in medical as diseased related to biochemistry changes of the cell and tissue. This paper reviewed the application of NIR spectroscopy in leukemia screening and in other medical application. General comparison between invasive and non-invasive NIR spectroscopy method is provided. The author also proposed a new non-invasive NIRS method in leukemia screening and compared it with the previous invasive NIRS method

Impact Modified Oil Palm Empty Fruit Bunch Fiber/Poly(Lactic) Acid Composite

In this study, composites were fabricated from oil palm empty fruit bunch fiber and poly(lactic) acid by extrusionn followed by injection moulding. Surface of the fiber was pre-treated by ultrasound in an alkali medium and treatment efficiency was investigated by scanning electron microscopy (SEM) analysis and Fourier transforms infrared spectrometer (FTIR). Effect of fiber treatment on composite was characterized by tensile strength (TS), tensile modulus (TM) and impact strength (IS). Furthermore, biostrong impact modifier was incorporated into the treated fiber composite to improve its impact properties. Mechanical testing showed an improvement of up to 23.5% and 33.6% respectively for TS and TM of treated fiber composite above untreated fiber composite. On the other hand incorporation of impact modifier led to enhancement of about 20% above the initial IS of the treated fiber composite

Analysis of the biceps brachii muscle by varying the arm movement level and load resistance band

Biceps brachii muscle illness is one of the common physical disabilities that requires rehabilitation exercises in order to build up the strength of the muscle after surgery. It is also important to monitor the condition of the muscle during the rehabilitation exercise through electromyography (EMG) signals. The purpose of this study was to analyse and investigate the selection of the best mother wavelet (MWT) function and depth of the decomposition level in the wavelet denoising EMG signals through the discrete wavelet transform (DWT) method at each decomposition level. In this experimental work, six healthy subjects comprised of males and females (26 ± 3.0 years and BMI of 22 ± 2.0) were selected as a reference for persons with the illness. The experiment was conducted for three sets of resistance band loads, namely, 5 kg, 9 kg, and 16 kg, as a force during the biceps brachii muscle contraction. Each subject was required to perform three levels of the arm angle positions (30°, 90°, and 150°) for each set of resistance band load. The experimental results showed that the Daubechies5 (db5) was the most appropriate DWT method together with a 6-level decomposition with a soft heursure threshold for the biceps brachii EMG signal analysis