Chemical composition and biological activities of Daucus aureus essential oils from Eastern Algeria

The aim of this study was to investigate the chemical composition of the essential oils of three populations of Daucus aureus from three sites in Eastern Algeria (Setif, Constantine and Oum Elbouaghi) and to test their antibacterial and antioxidant activities. The essential oils were obtained by hydrodistillation and analyzed by GC and GC/MS. The major components were sabinene (30.6% and 36.2%), n-nonane (8.0% and 7.9%), α-pinene (5.5% and 6.3%) and 4-terpineol (4.4% and 6.0%) in D. aureus from Setif and Constantine populations essential oils, respectively; whereas, α-pinene (19.4%), β-pinene (12.0%) and p-cymene (12.2%) were the major components in Oum Elbouaghi essential oil population. The chemical compositions of D. aureus from Eastern Algeria are markedly different from those from Western Algeria, and likely represent new chemotypes. The antimicrobial activity of the essential oils was evaluated against four bacteria and one fungus, using the disc-diffusion method and minimal inhibitory concentration (MIC), whereas, the antioxidant activity of the essential oils was evaluated using the DPPH test. The results showed that the oils have an antimicrobial activity against the microorganisms tested, with minimal inhibitory concentration (MIC) values between 0.97 and 3.23 mg/mL and weaker antioxidant and DPPH radical scavenging activities were found in comparison to butylated hydroxyl toluene (BHT)

Two new triterpenoid saponins from the leaves of Bupleurum lancifolium (Apiaceae)

Chemical investigation of the leaves of Bupleurum lancifolium led to the isolation and identification of two triterpenoid saponins previously undescribed named 3-O-[α-L-rhamnopyranosyl (1 → 4)-β-D-glucopyranosyl] echinocystic acid 28-O-β-D-glucopyranosyl ester (1) and 3-O-[α-L-rhamnopyranosyl (1 → 4)-β-D-glucopyranosyl] oleanolic acid 28-O-β-D-glucopyranosyl ester (2) along with the two known compounds isorhamnetin 3-rutinoside (3) and rutin (4). Their structures were elucidated by different spectroscopic methods, including HRESIMS analysis as well as 1D and 2D NMR experiments

Anti-AGEs Algerian vegetal extracts. Phytochemical study of Daucus aureus desf. (Apiaceae)

International audienc

Senecipyrrolidine, an unusual pyrrolidine alkaloid isolated from Jacobaea gigantea (Desf.) Pelser (Asteraceae)

Alkaloids and phenolic compounds are among the most biologically active natural products from the Jacobaea/Senecio genera (Asteraceae). To isolate original natural products directly from Jacobaea gigantea crude polar extracts, centrifugal partition chromatography (CPC) was used. Previously, we reported the phytochemical study of J. gigantea (syn. Senecio giganteus) n-butanol extract using various classical chromatographical techniques combined with CPC. Herein major constituents from the J. gigantea crude ethyl acetate extract and further compounds from the n-butanol extract were purified in only one step using this technique. A new pyrrolidine alkaloid, named senecipyrrolidine was isolated along with thirteen known compounds – chiro-inositol, three phenolic acids, six flavonoids, two quinones and emiline, another pyrrolidine alkaloid – from crude n-butanol or ethyl acetate extracts. Pyrrolidine alkaloids were isolated for the first time in the Jacobaea/Senecio genera and were probably biogenetically related to the two isolated quinones derivatives jacaranone and 3a-hydroxy-3,3a,7,7a-tetrahydrobenzofuran-2,6-dione, isolated in this species

Fast counter current chromatography of n-butanolic fraction from Senecio giganteus (Asteraceae)

Phenolic derivatives such as quinones, acid-phenols and flavonoids were successfully isolated from a n-butanolic fraction of Senecio giganteus Desf. (Asteraceae) flowers, namely jacaranone (1), 3a-hydroxy-3,3a,7,7a-tetrahydrobenzofuran-2,6-dione (2), chlorogenic acid (3), hyperoside (4), quercetin 3-O-β-D-robinobioside (5), isorhamnetin-3-O-β-D-glucuronide (6), quercetin-3-O-β-D-glucuronide (7), and isorhamnetin-3-O-β-D-glucuronide-6″-methyl ester (8). These compounds were purified through either classical polyamide filtration followed by fractionation on Si gel, or through fast centrifugal partition chromatography (FCPC). Using FCPC, the major compounds could be readily isolated from the crude n-butanolic fraction. Compounds 1-8 were identified by means of spectroscopic and spectrometric analysis (UV, 1H, 13C and 2D NMR, and MS). This work described for the first time the phytochemical composition of this endemic Algerian plant

Chemical composition of essential oil from Lavandula stoechas and Lavandula multifida growing wild in Algeria

The essential oils of the aerial parts of Lavandula multifida and L. stoechas were extracted at the full bloom stage by hydrodistillation and theirs chemical compositions were estimated by means of gas chromatography–mass spectrometry (GC–MS). A total of 46 and 67 constituents were identified representing 95.5% and 98.2% of the total oils, respectively. The main components of L. multifida oil were carvacrol (63.8%), beta-bisabolene (8.7%), spathulenol (6.2%), caryophyllene oxide (3.6%) and linalool (2.9%). The oil of L. stoechas was dominated by fenchone (63.9%), camphor (7.8%), 1,8-cineole (5.3%) and myrtenyl acetate (4.2%

Essential oil analysis of the aerial parts of Sideritis incana and Calamintha hispidula

The aerial parts of Sideritis incana and Calamintha hispidula at the flowering stage were submitted to hydrodistillation in a Clevenger–type apparatus for 3 hours and the chemical composition of the essential oil was analyzed by GC coupled to GC-MS. The essential oil contained a total of 99 constituents for S. incana and 31 for C. hispidula representing 95.7% and 99.6 of the total oils, rerspectively. The mains components of S. incana oil were linalool (25.2), cedrol (13.7%), geraniol (7%) and α-terpineol (5.4%). The chemical constituents of the oil from C. hispidula were predominated by pulegone (43.2%), isomenthone (36%), piperitone (3.2%), limonene (2.6%) and 4-terpineol (2.5%). The results revealed that the oil of the plants is characterized by the presence of many important components which could be applied in food, pharmaceutical and perfume industr