Line shape of the muH(3p - 1s) hyperfine transitions

The (3p - 1s) X-ray transition to the muonic hydrogen ground state was measured with a high resolution crystal spectrometer. A Doppler effect broadening of the X-ray line was established which could be attributed to different Coulomb de-excitation steps preceding the measured transition. The assumption of a statistical population of the hyperfine levels of the muonic hydrogen ground state was directly confirmed by the experiment and measured values for the hyperfine splitting can be reported. The results allow a decisive test of advanced cascade model calculations and establish a method to extract fundamental strong-interaction parameters from pionic hydrogen experiments.Comment: Submitted to Physical Review Letter

Line shape analysis of the Kβ\beta transition in muonic hydrogen

The K$\beta$ transition in muonic hydrogen was measured with a high-resolution crystal spectrometer. The spectrum is shown to be sensitive to the ground-state hyperfine splitting, the corresponding triplet-to-singlet ratio, and the kinetic energy distribution in the $3p$ state. The hyperfine splitting and triplet-to-singlet ratio are found to be consistent with the values expected from theoretical and experimental investigations and, therefore, were fixed accordingly in order to reduce the uncertainties in the further reconstruction of the kinetic energy distribution. The presence of high-energetic components was established and quantified in both a phenomenological, i.e. cascade-model-free fit, and in a direct deconvolution of the Doppler broadening based on the Bayesian approach.Comment: 22 pages, 21 figure

Pionic Deuterium

The strong interaction shift and broadening in pionic deuterium have been remeasured with high statistics by means of the (3p-1s) X-ray transition using the cyclotron trap and a high-resolution crystal spectrometer. Preliminary results are (-2325+/-31) meV (repulsive) for the shift and (1171+23/-49} meV for the width, which yields precise values for the pion-deuteron scattering length and the threshold parameter for pion production.Comment: Conf. Proc. Few Body 19 (FB19), August 31 - September 5, 2009, Bonn, Germany 9 pages, 13 figure

Precision determination of the dpi -> NN transition strength at threshold

An unusual but effective way to determine at threshold the dpi -> NN transition strength is to exploit the hadronic ground-state broadening in pionic deuterium, accessible by x-ray spectroscopy. The broadening is dominated by the true absorption channel dpi- -> nn, which is related to s-wave pion production pp -> dpi+ by charge symmetry and detailed balance. Using the exotic atom circumvents the problem of Coulomb corrections to the cross section as necessary in the production experiments. Our dedicated measurement finds (1171+23/-49) meV for the broadening yielding (252+5/-11) \mub.Comment: 4 pages, 2 figures, 1 tabl

Line shape of the μ H(3 p - 1 s ) transition

The line shape of the (3p − 1s) X-ray transition in muonic hydrogen was measured for the first time with a high-resolution crystal spectrometer. The assumption of a statistical population of the hyperfine levels was directly confirmed by experiment, and a measured value for the hyperfine splitting is reported. An X-ray line broadening due to Doppler effect could be clearly identified and attributed to different Coulomb de-excitation transitions which precede the measured radiative transition. The results allow a decisive test of advanced cascade model calculations and establish an alternative and "model free” method to extract the strong-interaction parameters from pionic hydrogen dat

The Rewiring of Ubiquitination Targets in a Pathogenic Yeast Promotes Metabolic Flexibility, Host Colonization and Virulence

Funding: This work was funded by the European Research Council [http://erc.europa.eu/], AJPB (STRIFE Advanced Grant; C-2009-AdG-249793). The work was also supported by: the Wellcome Trust [www.wellcome.ac.uk], AJPB (080088, 097377); the UK Biotechnology and Biological Research Council [www.bbsrc.ac.uk], AJPB (BB/F00513X/1, BB/K017365/1); the CNPq-Brazil [http://cnpq.br], GMA (Science without Borders fellowship 202976/2014-9); and the National Centre for the Replacement, Refinement and Reduction of Animals in Research [www.nc3rs.org.uk], DMM (NC/K000306/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments We thank Dr. Elizabeth Johnson (Mycology Reference Laboratory, Bristol) for providing strains, and the Aberdeen Proteomics facility for the biotyping of S. cerevisiae clinical isolates, and to Euroscarf for providing S. cerevisiae strains and plasmids. We are grateful to our Microscopy Facility in the Institute of Medical Sciences for their expert help with the electron microscopy, and to our friends in the Aberdeen Fungal Group for insightful discussions.Peer reviewedPublisher PD

Green Pathways for the Enzymatic Synthesis of Furan-Based Polyesters and Polyamides

The attention towards the utilization of sustainable feedstocks for polymer synthesis has grown exponentially in recent years. One of the spotlighted monomers derived from renewable resources is 2,5-furandicarboxylic acid (FDCA), one of the most promising bio-based monomers, due to its resemblance to petroleum-based terephthalic acid. Very interesting synthetic routes using this monomer have been reported in the last two decades. Combining the use of bio-based monomers and non-toxic chemicals via enzymatic polymerizations can lead to a robust and favorable approach towards a greener technology of bio-based polymer production. In this chapter, a brief introduction to FDCA-based monomers and enzymatic polymerizations is given, particularly focusing on furan-based polymers and their polymerization. In addition, an outline of the recent developments in the field of enzymatic polymerizations is discussed. </p

Transcriptional Profiling of Bacillus anthracis Sterne (34F2) during Iron Starvation

Lack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate within the host. Here we define the transcriptional response of B. anthracis Sterne (34F2) to iron depleted conditions. Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. Two genes encoding putative internalin proteins were chosen for further study. Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. This attenuation was amplified in a double mutant strain. These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study

A Small RNA Controls Expression of the Chitinase ChiA in Listeria monocytogenes

In recent years, more than 60 small RNAs (sRNAs) have been identified in the gram-positive human pathogen Listeria monocytogenes, but their putative roles and mechanisms of action remain largely unknown. The sRNA LhrA was recently shown to be a post-transcriptional regulator of a single gene, lmo0850, which encodes a small protein of unknown function. LhrA controls the translation and degradation of the lmo0850 mRNA by an antisense mechanism, and it depends on the RNA chaperone Hfq for efficient binding to its target. In the present study, we sought to gain more insight into the functional role of LhrA in L. monocytogenes. To this end, we determined the effects of LhrA on global-wide gene expression. We observed that nearly 300 genes in L. monocytogenes are either positively or negatively affected by LhrA. Among these genes, we identified lmo0302 and chiA as direct targets of LhrA, thus establishing LhrA as a multiple target regulator. Lmo0302 encodes a hypothetical protein with no known function, whereas chiA encodes one of two chitinases present in L. monocytogenes. We show here that LhrA acts as a post-transcriptional regulator of lmo0302 and chiA by interfering with ribosome recruitment, and we provide evidence that both LhrA and Hfq act to down-regulate the expression of lmo0302 and chiA. Furthermore, in vitro binding experiments show that Hfq stimulates the base pairing of LhrA to chiA mRNA. Finally, we demonstrate that LhrA has a negative effect on the chitinolytic activity of L. monocytogenes. In marked contrast to this, we found that Hfq has a stimulating effect on the chitinolytic activity, suggesting that Hfq plays multiple roles in the complex regulatory pathways controlling the chitinases of L. monocytogenes