Hemp (Cannabis sativa L.) as a Resource for Green Cosmetics: Yield of Seeds and Fatty Acids Composition of 20 Varieties under the Growing Conditions of Organic Farming in Austria

The interest in hemp (non-drug Cannabis sativa L.) for skin care and cosmetic use is due to the high content of oil, especially unsaturated fatty acids in seed with technological and therapeutic effects. In a field trial on an organic farm, seed weight and content of fatty acids of 20 hemp varieties were surveyed on three different harvest dates. The dry matter seed yields ranged from 27-149 g m2. The varieties Ferimon-12, Fedora-19, and Bialobreszie produced high seed yields on all three harvest dates but yields were not significantly different from a large group of other varieties. Contents of palmitic acid range from 3.1 to 4.1%, of stearic acid from 0.1 to 1.9%, of oleic acid from 3.7 to 9.2%, of linoleic acid from 44.8 to 60.2%, of α-linolenic acid from 18.2 to 27.4%, and of γ-linolenic acid from 1.6 to 4.7%. The genotype has no significant influence on fatty acid content. All 20 varieties tested show high quantities of fatty acid depending on the harvest date, so that no variety can be favored. Results confirm that hemp is a very good source of fatty acids for skin care and cosmetic use

Serodiagnosis of infectious mononucleosis by using recombinant Epstein-Barr virus antigens and enzyme-linked immunosorbent assay technology

Four recombinant, diagnostically useful Epstein-Barr virus (EBV) proteins representative of the viral capsid antigen (p150), diffuse early antigen (p54), the major DNA-binding protein (p138), and the EBV nuclear antigen (p72) (W. Hinderer, H. Nebel-Schickel, H.H. Sonneborn, M. Motz, R. Kühbeck, and H. Wolf, J. Exp. Clin. Cancer Res. 7[Suppl.]:132, 1988) were used to set up individual enzyme-linked immunosorbent assays (ELISAs) for the qualitative and quantitative detection of immunoglobulin M (IgM) and IgG antibodies. In direct comparison with results obtained by standard immunofluorescence or immunoperoxidase assays, it was then shown that the recombinant EBV ELISAs provide the means for specific and sensitive serodiagnosis of infectious mononucleosis (IM) caused by EBV. The most useful markers in sera from such patients proved to be IgM antibodies against p54, p138, and p150. Additional positive markers for recent or ongoing IM apparently were IgG antibodies against p54 and p138. In contrast, anti-p72 IgG had a high preference for sera from healthy blood donors and, therefore, can be considered indicative of past exposure to the virus. Altogether, the individual ELISAs proved to be as specific and at least as sensitive for the diagnosis of IM as the currently available standard techniques are. Moreover, our findings suggest that, by combining individual test antigens, a workable ELISA system consisting of three assays (IgM against p54, p138, and p150; IgG against p54 and p138; and IgG against p72) can be established for the standardized rapid diagnosis of acute EBV infections

Nonlinear spin-polarized transport through a ferromagnetic domain wall

A domain wall separating two oppositely magnetized regions in a ferromagnetic semiconductor exhibits, under appropriate conditions, strongly nonlinear I-V characteristics similar to those of a p-n diode. We study these characteristics as functions of wall width and temperature. As the width increases or the temperature decreases, direct tunneling between the majority spin bands decreases the effectiveness of the diode. This has important implications for the zero-field quenched resistance of magnetic semiconductors and for the design of a recently proposed spin transistor.Comment: 5 pages, 3 figure

HD 169142 in the eyes of ZIMPOL/SPHERE

We present new data of the protoplanetary disc surrounding the Herbig Ae/Be star HD 169142 obtained in the very broad-band (VBB) with the Zurich imaging polarimeter (ZIMPOL), a sub-system of the Spectro-Polarimetric High-contrast Exoplanet REsearch instrument (SPHERE) at the Very Large Telescope (VLT). Our Polarimetric Differential Imaging (PDI) observations probe the disc as close as 0.03" (3.5au) to the star and are able to trace the disc out to ~1.08" (~126au). We find an inner hole, a bright ring bearing substructures around 0.18" (21au), and an elliptically shaped gap stretching from 0.25" to 0.47" (29-55au). Outside of 0.47", the surface brightness drops off, discontinued only by a narrow annular brightness minimum at ~0.63"-0.74" (74-87au). These observations confirm features found in less-well resolved data as well as reveal yet undetected indications for planet-disc interactions, such as small-scale structures, star-disk offsets, and potentially moving shadows.Comment: Accepted for publication in MNRA

Studies on processing, particle formation, and immunogenicity of the HIV-1 gag gene product: a possible component of a HIV vaccine

Antigens in a particulate conformation were shown to be highly immunogenic in mammals. For this reason, the particle forming capacity of derivatives of the HIV-1 group specific core antigen p55 gag was assayed and compared dependent on various expression systems: recombinant bacteria, vaccinia- and baculoviruses were established encoding the entire core protein p55 either in its authentic sequence or lacking the myristylation consensus signal. Moreover, p55 gag was expressed in combination with the protease (p55-PR) or with the entire polymerase (p55-pol), respectively. Budding of 100-160 nm p55 core particles, resembling immature HIV-virions, was observed in the eucaryotic expression systems only. In comparison to the vaccinia virus driven expression of p55 in mammalian cells, considerably higher yields of particulate core antigen were obtained by infection of Spodoptera frugiperda (Sf9) insect cells with the recombinant Autographa californica nuclear polyhedrosis (AcMNPV) baculovirus. Mutation of the NH2-terminal myristylation signal sequence prevented budding of the immature core particles. Expression of the HIV p55-PR gene construct by recombinant baculovirus resulted in complete processing of the p55 gag precursor molecule in this system. The introduction of an artificial frameshift near the natural frameshift site resulted in constitutive expression of the viral protease and complete processing of p55, both in Escherichia coli and in vaccinia virus infected cells. Interestingly, significant processing of p55 resembling that of HIV infected H9 cells could also be achieved in the vaccinia system by fusing the entire pol gene to the gag gene. Moreover, processing was not found to be dependent on amino-terminal myristylation of the gag procursor molecule, which is in contrast to observations with type C and type D retrovirus. However, complete processing of p55 into p24, p17, p9 and p6 abolished particle formation. Purified immature HIV-virus like particles were highly immunogenic in rabbits, leading to a strong humoral immune response after immunization. Empty immature p55 gag particles represent a noninfectious and attractive candidate for a basic vaccine component