A longitudinal study of intonation in an a cappella singing quintet

Objective The skill to control pitch accurately is an important feature of performance in singing ensembles as it boosts musical excellence. Previous studies analyzing single performance sessions provide inconclusive and contrasting results on whether singers in ensembles tend to use a tuning system which deviates from equal temperament for their intonation. The present study observes the evolution of intonation in a newly formed student singing quintet during their first term of study. Methods/Design A semiprofessional singing quintet was recorded using head-worn microphones and electrolaryngograph electrodes to allow fundamental frequency (fo) evaluation of the individual voices. In addition, a camcorder was used to record verbal interactions between singers. The ensemble rehearsed a homophonic piece arranged for the study during five rehearsal sessions over four months. Singers practiced the piece for 10 minutes in each rehearsal, and performed three repetitions of the same pieces pre-rehearsal and post-rehearsal. Audio and electrolaryngograph data of the repeated performances, and video recordings of the rehearsals were analyzed. Aspects of intonation were then measured by extracting the fo values from the electrolaryngograph and acoustic signal, and compared within rehearsals (pre and post) and between rehearsals (rehearsals 1 to 5), and across repetitions (take 1 to 3). Time-stamped transcriptions of rehearsal discussions were used to identify verbal interactions related to tuning, the tuning strategies adopted, and their location (bar or chord) within the piece. Results/Discussion Tuning of each singer was closer to equal temperament than just intonation, but the size of major thirds was slightly closer to just intonation, and minor thirds closer to equal temperament. These findings were consistent within and between rehearsals, and across repetitions. Tuning was highlighted as an important feature of rehearsal during the study term, and a range of strategies were adopted to solve tuning related issues. This study provides a novel holistic assessment of tuning strategies within a singing ensemble, furthering understanding of performance practices as well as revealing the complex approach needed for future research in this area. These findings are particularly important for directors and singers to tailor rehearsal strategies that address tuning in singing ensembles, showing that approaches need to be context driven rather than based on theoretical ideal

A Longitudinal Study Investigating Synchronization in a Singing Quintet

Research suggests that synchronization between musicians during ensemble performances can be affected by the rhythmic or tonal complexity of the piece being performed and by group roles such as leader-follower relationships. Since previous studies have mostly been conducted within single performance sessions, developmental aspects of interpersonal synchronization in ensembles remain underinvestigated. This longitudinal study followed a newly formed singing ensemble from initial rehearsals to the performance stage in order to investigate the evolution of synchronization between advanced singing students during a university term of study in relation to the musical content of the piece and leader-follower relationships. An advanced postgraduate singing quintet was recorded using head-worn microphones and laryngograph electrodes to allow fundamental frequency evaluation of the individual voices. The quintet, formed to complete a 1-year Master’s programme in ensemble singing, rehearsed two pieces composed for the study, during five rehearsals over 3 months. Singers practised the same pieces in a randomized order across rehearsals and performed three repetitions of the same pieces before and after each rehearsal, resulting in six recordings per piece/rehearsal. Audio and laryngograph data of the repeated performances were collected, and synchronization was measured by extracting note times from the fundamental frequency values. The asynchronies of the two pieces before and after rehearsals were calculated and compared both within rehearsals (pre and post) and between rehearsals (rehearsals 1-5). Results demonstrate an increase in the precision of synchronization over the course of study, depending on the piece being rehearsed, and a more variable synchronization for the more rhythmically complex piece. Results also show changes in the distribution of the tendency to precede all co-performers across rehearsals, which became equally distributed among the musicians during the last rehearsal. The results reported here could have important implications for the tailoring of rehearsal strategies that could improve interpersonal synchronization between musicians during ensemble performances

Perception of synchronization in singing ensembles

Recent investigations analysing synchronization in singing ensembles have shown that the precision of synchronization during singing duo performances is better in the presence of visual contact between the singers than without. Research has also shown that synchronisation improves with practice across rehearsals in a newly formed singing quintet. However, whether listeners’ perceptions of synchronization reflect the different patterns of synchronization that were observed during ensemble performance with and without visual contact and across rehearsals has not yet been investigated. This study aims to analyse the perception of the synchronization associated with altered visual contact and amount of rehearsal during singing duo and quintet performances respectively, for listeners with different levels of musical expertise. A set of fifty-eight singing recordings selected from duo and quintet ensemble performances, collected from previous investigations of interpersonal synchrony, was presented to 33 listeners, including non-experts (university students with little or no music training), performers in the group (singers who performed the pieces used for the study), and other musicians (advanced music students). Participants were required to listen to each trial and judge the level of “togetherness” on a sliding scale from zero to 100. Results show that listeners, irrespective of their musical training and performance experience, perceived differences in the synchronization in the duo tokens depending on the presence/absence of visual contact between singers; on the other hand, the smaller asynchrony patterns measured across rehearsals in the singing quintet recordings were not perceived. This study contributes to our understanding of perceptions of synchronization by individuals with different levels of musical expertise, and underscores the perceptual salience of synchronization, regardless of musical experience

Group Singing as a Resource for the Development of a Healthy Public

A growing body of evidence points to a wide range of benefits arising from participation in group singing. Group singing requires participants to engage with each other in a simultaneous musical dialogue in a pluralistic and emergent context, creating a coherent cultural expression through the reflexive negotiation of (musical) meaning manifest in the collective power of the human voice. As such, group singing might be taken – both literally and figuratively – as a potent form of ‘healthy public’, creating an ‘ideal’ community which participants can subsequently mobilise as a positive resource for everyday life. The experiences of a group of singers (n=78) who had participated in an outdoor singing project were collected and analysed using a three-layer research design consisting of: distributed data generation and interpretation, considered against comparative data from other singing groups (n=88); a focus group workshop (n=11); an unstructured interview (n=2). The study confirmed an expected perception of the social bonding effect of group singing, highlighting affordances for interpersonal attunement and attachment alongside a powerful individual sense of feeling ‘uplifted’. This study presents a novel perspective on group singing, highlighting the importance of participant experience as a means of understanding music as a holistic and complex adaptive system. It validates findings about group singing from previous studies - in particular the stability of the social bonding effect as a less variant characteristic in the face of environmental and other situational influences, alongside its capacity for mental health recovery. It establishes a subjective sociocultural and musical understanding of group singing, by expanding on these findings to centralise the importance of individual experience, and the consciousness of that experience as descriptive self-awareness. The ways in which participants describe and discuss their experiences of group singing and its benefits points to a complex interdependence between a number of musical, neurobiological and psychosocial mechanisms which might be independently and objectively analysed. An emerging theory is that at least some of the potency of group singing is as a resource where people can rehearse and perform ‘healthy’ relationships, further emphasising its potential as a resource for healthy publics

Allergens induce enhanced bronchoconstriction and leukotriene production in C5 deficient mice

BACKGROUND: Previous genetic analysis has shown that a deletion in the complement component 5 gene-coding region renders mice more susceptible to allergen-induced airway hyperresponsiveness (AHR) due to reduced IL-12 production. We investigated the role of complement in a murine model of asthma-like pulmonary inflammation. METHODS: In order to evaluate the role of complement B10 mice either sufficient or deficient in C5 were studied. Both groups of mice immunized and challenged with a house dust extract (HDE) containing high levels of cockroach allergens. Airways hyper-reactivity was determined with whole-body plesthysmography. Bronchoalveolar lavage (BAL) was performed to determine pulmonary cellular recruitment and measure inflammatory mediators. Lung homogenates were assayed for mediators and plasma levels of IgE determined. Pulmonary histology was also evaluated. RESULTS: C5-deficient mice showed enhanced AHR to methylcholine challenge, 474% and 91% increase above baseline Penh in C5-deficient and C5-sufficient mice respectively, p < 0.001. IL-12 levels in the lung homogenate (LH) were only slightly reduced and BAL IL-12 was comparable in C5-sufficient and C5-deficient mice. However, C5-deficient mice had significantly higher cysteinyl-leukotriene levels in the BAL fluid, 1913 +/- 246 pg/ml in C5d and 756 +/- 232 pg/ml in C5-sufficient, p = 0.003. CONCLUSION: These data demonstrate that C5-deficient mice show enhanced AHR due to increased production of cysteinyl-leukotrienes

Anaphylatoxin C3a receptors in asthma

The complement system forms the central core of innate immunity but also mediates a variety of inflammatory responses. Anaphylatoxin C3a, which is generated as a byproduct of complement activation, has long been known to activate mast cells, basophils and eosinophils and to cause smooth muscle contraction. However, the role of C3a in the pathogenesis of allergic asthma remains unclear. In this review, we examine the role of C3a in promoting asthma. Following allergen challenge, C3a is generated in the lung of subjects with asthma but not healthy subjects. Furthermore, deficiency in C3a generation or in G protein coupled receptor for C3a abrogates allergen-induced responses in murine models of pulmonary inflammation and airway hyperresponsiveness. In addition, inhibition of complement activation or administration of small molecule inhibitors of C3a receptor after sensitization but before allergen challenge inhibits airway responses. At a cellular level, C3a stimulates robust mast cell degranulation that is greatly enhanced following cell-cell contact with airway smooth muscle (ASM) cells. Therefore, C3a likely plays an important role in asthma primarily by regulating mast cell-ASM cell interaction

Exploring the potential of virtual reality technology to investigate the health and well being benefits of group singing

There is a growing body of academic research aiming to quantify and understand the associated health and well being benefits of group singing. The social interaction is known to strongly contribute to perceived improvements to mental and physical health but there are also indications that singing together elicits better well being outcomes that other community activities. This paper introduces the Vocal Interaction in an Immersive Virtual Acoustic (VIIVA) system, which allows the user to take part in a group singing activity in 360 degree virtual reality, hearing themselves in the recorded venue alongside the other singers. The VIIVA is intended to make group singing accessible to those unable to attend real community choirs but also as a tool for experimental research into the health and well being benefits of group singing. This paper describes the system and presents a number of methodologies and applications which are discussed in relation to three ongoing research projects. Preliminary work indicates that the VIIVA system and the devised setups provide a promising tool with which to study the health and well being benefits of group singing, and in particular to control for the social interactions inherent in real group singing activities

Identifying Key Factors Associated with Aggression on Acute Inpatient Psychiatric Wards

Aggressive behaviour is a critical issue for modern acute psychiatric services, not just because of the adverse impact it has on patients and staff, but also because it puts a financial strain on service providers. The aim of this study was to assess the relationship of patient violence to other variables: patient characteristics, features of the service and physical environment, patient routines, staff factors, the use of containment methods, and other patient behaviours. A multivariate cross sectional design was utilised. Data were collected for a six month period on 136 acute psychiatric wards in 26 NHS Trusts in England. Multilevel modelling was conducted to ascertain those factors most strongly associated with verbal aggression, aggression toward objects, and physical aggression against others. High levels of aggression were associated with a high proportion of patients formally detained under mental health legislation, high patient turnover, alcohol use by patients, ward doors being locked, and higher staffing numbers (especially qualified nurses). The findings suggest that the imposition of restrictions on patients exacerbates the problem of violence, and that alcohol management strategies may be a productive intervention. Insufficient evidence is available to draw conclusions about the nature of the link between staffing numbers and violence

Targeting IL-1β and IL-17A driven inflammation during influenza-induced exacerbations of chronic lung inflammation.

For patients with chronic lung diseases, such as chronic obstructive pulmonary disease (COPD), exacerbations are life-threatening events causing acute respiratory distress that can even lead to hospitalization and death. Although a great deal of effort has been put into research of exacerbations and potential treatment options, the exact underlying mechanisms are yet to be deciphered and no therapy that effectively targets the excessive inflammation is available. In this study, we report that interleukin-1β (IL-1β) and interleukin-17A (IL-17A) are key mediators of neutrophilic inflammation in influenza-induced exacerbations of chronic lung inflammation. Using a mouse model of disease, our data shows a role for IL-1β in mediating lung dysfunction, and in driving neutrophilic inflammation during the whole phase of viral infection. We further report a role for IL-17A as a mediator of IL-1β induced neutrophilia at early time points during influenza-induced exacerbations. Blocking of IL-17A or IL-1 resulted in a significant abrogation of neutrophil recruitment to the airways in the initial phase of infection or at the peak of viral replication, respectively. Therefore, IL-17A and IL-1β are potential targets for therapeutic treatment of viral exacerbations of chronic lung inflammation