The utility of PSMA and PSA immunohistochemistry in the cytologic diagnosis of metastatic prostate carcinoma

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/107502/1/dc23075.pd

Shocked Molecular Gas in the Supernova Remnant HB 21

We report the discovery of the shocked molecular gas in the supernova remnant HB 21. We derive the physical parameters of the shocked gas from CO J=1-0 and J=2-1 line observations. We discuss the correlation of the shocked molecular gas with the previously detected, shocked atomic gas and the associated infrared emission.Comment: 24 pages, 10 figures, To appear in the ApJ, scheduled for the April 10, 2001 issue (v551

Amino-functionalized magnetic chitosan beads to enhance immobilization of potassium copper hexacyanoferrate for selective Cs+ removal and facile recovery

Potassium copper hexacyanoferrate (KCuHCF)-incorporated magnetic chitosan beads (HMC) were synthesized for both selective Cs+ removal in aqueous solutions and facile recovery of the spent adsorbent. To disperse and immobilize large amounts of the KCuHCF, methyl acrylate and diethylenetriamine were sequentially grafted onto the one-step synthesized magnetic chitosan beads. The additional introduction of amino functionality led to the enriched Cu2+ ions on the bead surface to incorporate KCuHCF into the grafting matrix. Consequently, the HMC exhibited a high Cs+ capacity calculated to be 136.47 mg g−1 from the Langmuir model, and the equilibrium was established within 4 h. Moreover, the HMC exhibited excellent stability in a wide pH range from 4 to 11 and an outstanding Cs+ selectivity (>97%) in seawater (1.11 mg L−1 Cs+). From a practical point of view, the HMC was stable during five successive adsorption cycles and easily recovered by magnets, enabling continuous operation to decontaminate a large volume of wastewater

Metal Reduction at Cold Temperatures by Shewanella Isolates from Various Marine Environments

Members of the genus Shewanella capable of reducing metals and forming minerals under cold-temperature conditions were isolated from 3 distinct marine habitats (the coast of Wash- ington State, the Puget Sound, and an iron-rich microbial mat off Hawaii). Cultures of microorgan- isms were isolated at 8°C on nutrient agar medium prepared in artificial seawater. Isolates in this study could use a wide variety of electron acceptors such as oxygen, nitrate, and metals, and reduce various metals coupled to the oxidation of several organic acids, glucose or hydrogen at temperatures down to 0°C. Akaganeite was reduced to either magnetite or siderite, depending on the test condi- tions. The geochemical profiles at the sample sites from which these strains were isolated spanned a temperature range of 1.8 to 11°C, and all showed active oxygen and nitrate reduction as well as metal reduction. This confirms previous reports that sediment microorganisms participating in biogeo- chemical cycles remain active at low temperatures

Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis

The original publication is available at www.springerlink.com.ArticleMOLECULAR AND CELLULAR BIOCHEMISTRY. 276(1-2): 133-141 (2005)journal articl

Current quark mass effects on chiral phase transition of QCD in the improved ladder approximation

Current quark mass effects on the chiral phase transition of QCD is studied in the improved ladder approximation. An infrared behavior of the gluon propagator is modified in terms of an effective running coupling. The analysis is based on a composite operator formalism and a variational approach. We use the Schwinger-Dyson equation to give a ``normalization condition'' for the Cornwall-Jackiw-Tomboulis effective potential and to isolate the ultraviolet divergence which appears in an expression for the quark-antiquark condensate. We study the current quark mass effects on the order parameter at zero temperature and density. We then calculate the effective potential at finite temperature and density and investigate the current quark mass effects on the chiral phase transition. We find a smooth crossover for $T>0$, $\mu=0$ and a first-order phase transition for $\mu>0$, T=0. Critical exponents are also studied and our model gives the classical mean-field values. We also study the temperature dependence of masses of scalar and pseudoscalar bosons. A critical end point in the $T$-$\mu$ plane is found at $T \sim 100$ MeV, $\mu \sim 300$ MeV.Comment: 19 pages, 13 figure

2021 occultations and transits of Linus orbiting (22) Kalliope: I. Polygonal and `cliptracing' algorithm

The satellite Linus orbiting the main-belt asteroid (22) Kalliope exhibited occultation and transit events in late 2021. A photometric campaign was organized and observations were taken by the TRAPPIST-South, SPECULOOS-Artemis, OWL-Net, and BOAO telescopes, with the goal to constrain models of this system. Our dynamical model is complex, with multipoles (up to the order $\ell = 2$), internal tides, and external tides. The model was constrained by astrometry (spanning 2001--2021), occultations, adaptive-optics imaging, calibrated photometry, as well as relative photometry. Our photometric model was substantially improved. A new precise (${<}\,0.1\,{\rm mmag}$) light curve algorithm was implemented, based on polygon intersections, which are computed exactly -- by including partial eclipses and partial visibility of polygons. Moreover, we implemented a `cliptracing' algorithm, based again on polygon intersections, in which partial contributions to individual pixels are computed exactly. Both synthetic light curves and synthetic images are then very smooth. Based on our combined solution, we confirmed the size of Linus, $(28\pm 1)\,{\rm km}$. However, this solution exhibits some tension between the light curves and the PISCO speckle-interferometry dataset. In most solutions, Linus is darker than Kalliope, with the albedos $A_{\rm w} = 0.40$ vs. $0.44$. This is confirmed on deconvolved images. A~detailed revision of astrometric data allowed us to revise also the $J_2 \equiv -C_{20}$ value of Kalliope. Most importantly, a~homogeneous body is excluded. For a differentiated body, two solutions exist: low-oblateness ($C_{20} \simeq -0.12$), with a~spherical iron core, and alternatively, high-oblateness ($C_{20} \simeq -0.22$) with an elongated iron core. These correspond to the low- and high-energy collisions, respectively, studied by means of SPH simulations in our previous work.Comment: Astronomy and Astrophysics, accepte

A literature review on surgery for cervical vagal schwannomas

Cervical vagal schwannoma is a benign, slow-growing mass, often asymptomatic, with a very low lifetime risk of malignant transformation in general population, but diagnosis is still a challenge. Surgical resection is the treatment of choice even if its close relationship with nerve fibres, from which it arises, threats vagal nerve preservation. We present a case report and a systematic review of literature. All studies on surgical resection of cervical vagal schwannoma have been reviewed. Papers matching the inclusion criteria (topic on surgical removal of cervical vagal schwannoma, English language, full text available) were selected. Fifty-three patients with vagal neck schwannoma submitted to surgery were identified among 22 studies selected. Female/male ratio was 1.5 and median age 44 years. Median diameter was 5 cm (range 2 to 10). Most schwannoma were asymptomatic (68.2%) and received an intracapsular excision (64.9%). Postoperative symptoms were reported in 22.6% of patients. Cervical vagal schwannoma is a benign pathology requiring surgical excision, but frequently postoperative complications can affect patients lifelong, so, surgical indications should be based carefully on the balance between risks and benefits

An initial event in insect innate immune response: structural and biological studies of interactions between β-1,3-glucan and the N-terminal domain of β-1,3-glucan recognition protein

In response to invading microorganisms, insect β-1,3-glucan recognition protein (βGRP), a soluble receptor in the hemolymph, binds to the surfaces of bacteria and fungi and activates serine protease cascades that promote destruction of pathogens by means of melanization or expression of antimicrobial peptides. Here we report on the NMR solution structure of the N-terminal domain of βGRP (N-βGRP) from Indian meal moth (Plodia interpunctella), which is sufficient to activate the prophenoloxidase (proPO) pathway resulting in melanin formation. NMR and isothermal calorimetric titrations of N-βGRP with laminarihexaose, a glucose hexamer containing β-1,3 links, suggest a weak binding of the ligand. However, addition of laminarin, a glucose polysaccharide (~ 6 kDa) containing β-1,3 and β-1,6 links that activates the proPO pathway, to N-βGRP results in the loss of NMR cross-peaks from the backbone 15N-1H groups of the protein, suggesting the formation of a large complex. Analytical ultra centrifugation (AUC) studies of formation of N-βGRP:laminarin complex show that ligand-binding induces sel-fassociation of the protein:carbohydrate complex into a macro structure, likely containing six protein and three laminarin molecules (~ 102 kDa). The macro complex is quite stable, as it does not undergo dissociation upon dilution to sub-micromolar concentrations. The structural model thus derived from the present studies for N-βGRP:laminarin complex in solution differs from the one in which a single N-βGRP molecule has been proposed to bind to a triple helical form of laminarin on the basis of an X-ray crystallographic structure of N-βGRP:laminarihexaose complex [Kanagawa, M., Satoh, T., Ikeda, A., Adachi, Y., Ohno, N., and Yamaguchi, Y. (2011) J. Biol. Chem. 286, 29158-29165]. AUC studies and phenoloxidase activation measurements carried out with the designed mutants of N-βGRP indicate that electrostatic interactions involving Asp45, Arg54, and Asp68 between the ligand-bound protein molecules contribute in part to the stability of N-βGRP:laminarin macro complex and that a decreased stability is accompanied by a reduced activation of the proPO pathway. Increased β-1,6 branching in laminarin also results in destabilization of the macro complex. These novel findings suggest that ligand-induced self-association of βGRP:β-1,3-glucan complex may form a platform on a microbial surface for recruitment of downstream proteases, as a means of amplification of the initial signal of pathogen recognition for the activation of the proPO pathway