Markers of automaticity in sleep-associated consolidation of novel words

Two experiments investigated effects of sleep on consolidation and integration of novel form-meaning mappings using size congruity and semantic distance paradigms. Both paradigms have been used in previous studies to measure automatic access to word meanings. When participants compare semantic or physical font size of written word-pairs (e.g. BEE–COW), judgments are typically faster if relative sizes are congruent across both dimensions. Semantic distance effects are also found for wellestablished words, with semantic size judgements faster for pairs that differ substantially on this dimension. English-speaking participants learned novel form-meaning mappings with Mandarin (Experiment 1) or Malay (Experiment 2) words and were tested following overnight sleep or a similar duration awake. Judgements on English words controlled for circadian effects. The sleep group demonstrated selective stronger size congruity and semantic distance effects for novel word-pairs. This benefit occurred in Experiment 1 for semantic size comparisons of novel words, and in Experiment 2 on comparisons where novel pairs had large distances and font differences (for congruity effects) or in congruent trials (for semantic distance effects). Conversely, these effects were equivalent across sleep and wake for English words. Experiment 2 included polysomnography data and revealed that changes in the strength of semantic distance and congruity effects were positively correlated with slow-wave sleep and sleep spindles respectively. These findings support systems consolidation accounts of declarative learning and suggest that sleep plays an active role in integrating new words with existing knowledge, resulting in increased automatic access of the acquired knowledge

Regulation of the cardiomyocyte transcriptome vs translatome by endothelin-1 and insulin: translational regulation of 5' terminal oligopyrimidine tract (TOP) mRNAs by insulin

Background: Changes in cellular phenotype result from underlying changes in mRNA transcription and translation. Endothelin-1 stimulates cardiomyocyte hypertrophy with associated changes in mRNA/protein expression and an increase in the rate of protein synthesis. Insulin also increases the rate of translation but does not promote overt cardiomyocyte hypertrophy. One mechanism of translational regulation is through 5' terminal oligopyrimidine tracts (TOPs) that, in response to growth stimuli, promote mRNA recruitment to polysomes for increased translation. TOP mRNAs include those encoding ribosomal proteins, but the full panoply remains to be established. Here, we used microarrays to compare the effects of endothelin-1 and insulin on the global transcriptome of neonatal rat cardiomyocytes, and on mRNA recruitment to polysomes (i.e. the translatome). Results: Globally, endothelin-1 and insulin (1 h) promoted >1.5-fold significant (false discovery rate 1.25-fold significant changes in expression in total and/or polysomal RNA induced by endothelin-1 or insulin, respectively, of which ~35% of endothelin-1-responsive and ~56% of insulin-responsive transcripts were translationally regulated. Of mRNAs for established proteins recruited to polysomes in response to insulin, 49 were known TOP mRNAs with a further 15 probable/possible TOP mRNAs, but 49 had no identifiable TOP sequences or other consistent features in the 5' untranslated region. Conclusions: Endothelin-1, rather than insulin, substantially affects global transcript expression to promote cardiomyocyte hypertrophy. Effects on RNA recruitment to polysomes are subtle, with differential effects of endothelin-1 and insulin on specific transcripts. Furthermore, although insulin promotes recruitment of TOP mRNAs to cardiomyocyte polysomes, not all recruited mRNAs are TOP mRNAs

Energy Balance of Laying Hens under Different Climates

A series of short term (3-4 weeks) energy balance experiments on laying hens were carried out under temperate and tropical climates. The results indicated that hens on ME restriction could produce egg while at the same time maintained positive energy balance. This was possible because nutrient requirements to maintain higher body weight which resulted from energy restriction, could have been reduced. Total heat production data obtained for temperate and tropical climates were 172 and 187 kcal(kgo.75 respectively

Wave Propagation in Lossy and Superconducting Circular Waveguides

We present an accurate approach to compute the attenuation of waves, propagating in circular waveguides with lossy and superconducting walls. A set of transcendental equation is developed by matching the fields at the surface of the wall with the electrical properties of the wall material. The propagation constant kz is found by numerically solving for the root of the equation. The complex conductivity of the superconductor is obtained from the Mattis-Bardeen equations. We have compared the loss of TE11 mode computed using our technique with that using the perturbation and Stratton’s methods. The results from the three methods agree very well at a reasonable range of frequencies above the cutoff. The curves, however, deviate below cutoff and at millimeter wave frequencies. We attribute the discrepancies to the dispersive effect and the presence of the longitudinal fields in a lossy waveguide. At frequencies below the gap, the superconducting waveguide exhibits lossless transmission behavior. Above the gap frequency, Cooper-pair breaking becomes dominant and the loss increases significantly

Who Researches Functional Literacy?

The purpose of our study was to discover who researches functional literacy. This study was situated within a larger systematic literature review. We searched seven electronic databases and identified 90 sources to answer our larger question regarding how functional literacy is defined and conceptualized as well as the specific question pertinent to this paper. An analytic template guided our data collection. The analysis was completed by using a spreadsheet to examine two levels of institutional affiliation: 1) the larger institution such as a university or government unit, and 2) the department, program, or division within the institution. Findings showed the 209 authors of these 90 sources represent a diverse set of fields. Further, it appears that multidisciplinary research is being conducted. We discuss implications of these findings and call for more interdisciplinary research

Nuclear Dbf2-related protein kinases (NDRs) in isolated cardiac myocytes and the myocardium: activation by cellular stresses and by phosphoprotein serine-/threonine-phosphatase inhibitors

The nuclear Dbf2-related protein kinases 1 and 2 (NDR1/2) are closely-related AGC family kinases that are strongly conserved through evolution. In mammals, they are activated inter alia by phosphorylation of an hydrophobic domain threonine-residue [NDR1(Thr-444)/NDR2(Thr-442)] by an extrinsic protein kinase followed by autophosphorylation of a catalytic domain serine-residue [NDR1(Ser-281)/NDR2(Ser-282)]. We examined NDR1/2 expression and regulation in primary cultures of neonatal rat cardiac myocytes and in perfused adult rat hearts. In myocytes, transcripts for NDR2, but not NDR1, were induced by the hypertrophic agonist, endothelin-1. NDR1(Thr-444) and NDR2(Thr-442) were rapidly phosphorylated (maximal in 15-30 min) in myocytes exposed to some phosphoprotein Ser-/Thr-phosphatase 1/2 inhibitors (calyculin A, okadaic acid) and, to a lesser extent, by hyperosmotic shock, low concentrations of H(2)O(2), or chelerythrine. In myocytes adenovirally-transduced to express FLAG-NDR2 (which exhibited a mainly-cytoplasmic localisation), the same agents increased FLAG-NDR2 activity as assessed by in vitro protein kinase assays, indicative of FLAG-NDR2(Ser-282/Thr-442) phosphorylation. Calyculin A-induced phosphorylation of NDR1(Thr-444)/NDR2(Thr-442) and activation of FLAG-NDR2 were inhibited by staurosporine, but not by other protein kinase inhibitors tested. In ex vivo rat hearts, NDR1(Thr-444)/NDR2(Thr-442) were phosphorylated in response to ischaemia-reperfusion or calyculin A. From a pathological viewpoint, we conclude that activities of NDR1 and NDR2 are responsive to cytotoxic stresses in heart preparations and this may represent a previously-unidentified response to myocardial ischaemia in vivo

Exploring the action landscape with trial world-lines

The Hamilton action principle, also known as the principle of least action, and Lagrange equations are an integral part of advanced undergraduate mechanics. At present, substantial efforts are ongoing to suitably incorporate the action principle in introductory physics courses. Although the Hamilton principle is oft stated as "the action for any nearby trial world-line is greater than the action for the classical world-line", the landscape of action in the space of world-lines is rarely explored. Here, for three common problems in introductory physics - a free particle, a uniformly accelerating particle, and a simple harmonic oscillator - we present families of trial world-lines, characterized by a few parameters, that evolve continuously from their respective classical world-lines. With explicit analytical expressions available for the action, they permit a graphical visualization of the action landscape in the space of nearby world-lines. Although these trial world-lines form only a subset of the space of all nearby world-lines, they provide a pedagogical tool that complements the traditional Lagrange equation approach and is well-suited for advanced undergraduate students.Comment: 9 pages, 6 figures, significant structural revisio

Attenuation in Rectangular Waveguides with Finite Conductivity Walls

We present a fundamental and accurate approach to compute the attenuation of electromagnetic waves propagating in rectangular waveguides with finite conductivity walls. The wavenumbers kx and ky in the x and y directions respectively, are obtained as roots of a set of transcendental equations derived by matching the tangential component of the electric field (E) and the magnetic field (H) at the surface of the waveguide walls. The electrical properties of the wall material are determined by the complex permittivity ε, permeability μ, and conductivity σ. We have examined the validity of our model by carrying out measurements on the loss arising from the fundamental TE10 mode near the cutoff frequency. We also found good agreement between our results and those obtained by others including Papadopoulos’ perturbation method across a wide range of frequencies, in particular in the vicinity of cutoff. In the presence of degenerate modes however, our method gives higher losses, which we attribute to the coupling between modes as a result of dispersion