Evaluation of Hungarian Wines for Resveratrol by Overpressured Layer Chromatography

A method, including solid phase extraction sample preparation, overpressured layer chromatographic separation and subsequent densitometric evaluation, was developed for measurement of total resveratrol (cis- and trans-isomers) content of wine. The amount of resveratrol was determined in wine samples from different winemaking regions of Hungary. The total resveratrol was high in Hungarian red wines (3.6–11 mg/L), and much lower in white ones (0.04–1.5 mg/L)

Badania nad otrzymywaniem produktów granulowanych

Granular protein products whi.ch can resemble fish roe and which may be used as car.r,iers of flavouring substan,ce for meat and fruit aroma respectiveli, are made by dropping of deaerated protein suspension on hot vegetable oil. The main ingredients are dried bovine blood plasma, dried wheat gluten and egg protein. The influence of these ingriedients of following functional properties were investigated: viscosity of the protein suspension, their ability to form droplets, th,e firmness and sphere-shape of the formed protein bodies. The results show that the production of desirable texture characteristics is possible aind that bovine blood plasma influences greatly the firmness of the protein bodies after a short heating time; vital gluten increases this firmness after a long heat treatment. Blood plasma shows a positive effect on the sphere-shape of the protein bodies and favours the simulating of fish roe.Produkty białkowe zgranulowane przypominające ikrę rybią, które mogą być użyte jako nośniki substancji aromatycznych do mięsa i aromatu owoców zostały wykonane przez wpuszczanie odpowietrzonej zawiesiny białka do gorącego oleju roślinnego. Głównym składnikiem jest suszona plazma krwi wołowej, suszony gluten i białko jaja. Badano wpływ tych składników na właściwości funkcjonalne, a mianowicie: lepkość zawiesiny białkowej, zdolność jej do tworzenia kropli, twardość i tendencja do utrzymywania kształtu utworzonych tworów białkowych. Wyniki wskazują, że wytwarzanie tekstury o pożądanej charakterystyce jest możliwe i że wołowa plazma krwi wpływa w bardzo dużym stopniu na twardość ciał białkowych po krótkim czasie ogrzewania, a witalny gluten zwiększa twardość po dłuższym okresie ogrzewania. Plazma krwi daje pozytywny efekt odnośnie utrzymywania kształtu ciał białkowych i sprzyja upodobnianiu się do ikry rybiej

An open source based high content screening method for cell biology laboratories investigating cell spreading and adhesion

BACKGROUND: Adhesion dependent mechanisms are increasingly recognized to be important for a wide range of biological processes, diseases and therapeutics. This has led to a rising demand of pharmaceutical modulators. However, most currently available adhesion assays are time consuming and/or lack sensitivity and reproducibility or depend on specialized and expensive equipment often only available at screening facilities. Thus, rapid and economical high-content screening approaches are urgently needed. RESULTS: We established a fully open source high-content screening method for identifying modulators of adhesion. We successfully used this method to detect small molecules that are able to influence cell adhesion and cell spreading of Swiss-3T3 fibroblasts in general and/or specifically counteract Nogo-A-Δ20-induced inhibition of adhesion and cell spreading. The tricyclic anti-depressant clomipramine hydrochloride was shown to not only inhibit Nogo-A-Δ20-induced cell spreading inhibition in 3T3 fibroblasts but also to promote growth and counteract neurite outgrowth inhibition in highly purified primary neurons isolated from rat cerebellum. CONCLUSIONS: We have developed and validated a high content screening approach that can be used in any ordinarily equipped cell biology laboratory employing exclusively freely available open-source software in order to find novel modulators of adhesion and cell spreading. The versatility and adjustability of the whole screening method will enable not only centers specialized in high-throughput screens but most importantly also labs not routinely employing screens in their daily work routine to investigate the effects of a wide range of different compounds or siRNAs on adhesion and adhesion-modulating molecules