Person-organization Fit, Kepuasan Kerja, Dan Turnover Intention: Studi Empiris Pada Karyawan Generasi Y Industri Perbankan Di Indonesia

The object of this study is to analyze the influence of person-organization fit toward turnover intention and job satisfaction as mediating variable in the Generation Y banking industry employees in Indonesia. The sample used in this study was 62 people who are classified as Generation Y. Results from this study indicate that thevariable person-organization fit has a positive and significant impact on job satisfaction. Job satisfaction variables also have a significant negative effect toward turnover intention. In addition this study, shows that the influence of person-organization fit toward turnover intention is mediated by job satisfaction

Penggunaan Internet Of Things (IoT) untuk Pemantauan dan Pengendalian Sistem Hidroponik

Internet of things (IoT) sedang marak digunakan dalam perkembangan teknologi saat ini. IoT dapat diartikan sebagai komunikasi antara satu perangkat dengan perangkat lain menggunakan internet. Kemajuan teknologi IoT ini dapat memudahkan berbagai macam pekerjaan, termasuk dalam pengendalian sistem hidroponik, sehingga perawatan tanaman dapat dilakukan dari jarak jauh dan setiap waktu. Hidroponik merupakan solusi terbaik untuk masyarakat di ibukota yang minim lahan untuk penghijauan. Komponen yang dibutuhkan dalam IoT antara lain perangkat yang mempunyai modul IoT, perangkat koneksi ke Internet seperti modem dan router, dan sebuah basis data tempat semuanya terkumpul. Tujuan dari penelitian ini adalah memanfaatkan teknologi IoT untuk memantau dan mengendalikan kondisi tanaman pada sistem hidroponik dari jarak jauh. Pengolahan hasil sensor dari mikrokontroler end device akan dikirim melalui XBee ke mikrokontroler server dan ditampilkan ke web server ThingSpeak. Sebuah aplikasi dibuat untuk smartphone yang terkoneksi dengan ThingSpeak yang dapat memantau dan mengontrol sistem kapan saja dan di mana saja. Kontrol yang dilakukan akan mengirim logika satu atau nol ke ThingSpeak dan diteruskan ke perangkat yang digunaka

Pengaruh Komunikasi Interpersonal Mahasiswa Dan Dosen Pembimbing Skripsi Terhadap Gejala Stres Mahasiswa Dalam Menyusun Skripsi

This study was conducted to determine the influence of interpersonal communication between students and the thesis supervisor students in the stress symptoms thesis. Based on initial observations on Communication department students of Dehasen University year 2010, of the 62 students who have submitted a title and run the thesis counseling process, only 29 students managed to reach the stage of proposal examination. One of the obstacles faced by students is the lack of good interpersonal communication so that interpersonal relationships are not well established. This research is a quantitative descriptive. Sample set by using purposive sampling of the students of Communication Department who were working on thesis, that is 38 students. The results showed that interpersonal communication between students and thesis supervisor has not been effective so that the discussion does not go well. The ineffectiveness of communication activities lead students to experience stress symptoms so that interfere the completion of thesis writing

Situasi Filariasis Setelah Pengobatan Massal Tahun Ketiga di Kabupaten Mamuju Utara

Mass Drug Administration (MDA) of lymphatic filariasis has been carried out for three years in North of Mamuju District. However, achievement of efficacy of this programme was unknown. The implementation of mass treatment to filariasis have been conducted for three years in the District of North Mamuju, but achievement of efficacy of this activity were not known yet. To determine the change of filarial situation as well as knowledge, attitude and behavior of the people to filariasis, after three years mass drug treatment, studies were conducted in March-November 2015, the activities were to collect mass blood fingerprick and interview to the local community. The blood fingerprick was conducted to two selected villages do to age 5 years up old (≥ 5 years), and interviewed was conducted to thirty selected villages do to age 15 years up old (≥ 15 years). The results showed that microfilaria rate in North Mamuju district was 1,39%, and the species was Brugiamalayi. Interviewed to 1,586 respondents indicated that knowledge of filariasis disease and the mass treatment it self were low, similary with the behavior related prevention to drug consumption. On the other hand their behave to prevention, control and filariasis treatment were positif. Microfilaria rate was still higher than 1%, as well as knowledge, attitude and behavior related to prevention and mass drug consumption were particularly still low, those indicated that after three years of mass drug treatment implementation the result did not showed as excpected. It suggested that mass drug implementation in North Mamuju need to be continued until five years,with right procedures and seriously monitoring to the area with chronic and microfilaria positive cases

PENGARUH KOMITMEN ORGANISASI DAN LINGKUNGAN KERJA TERHADAP KINERJA KARYAWAN AMANDA BROWNIES BANDUNG (AMANDA PUSAT)

ABSTRAK Permasalahan ditemukan pada Amanda Brownies Bandung (Amanda Pusat) mengenai rendahnya tingkat kinerja karyawan yang diakibatkan oleh komitmen organisasi dan lingkungan kerja yang masih rendah. Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh komitmen organisasi dan lingkungan kerja terhadap kinerja karyawan Amanda Brownies Bandung (Amanda Pusat) baik secara parsial maupun simultan. Metode penelitian yang digunakan adalah deskriptif dan verifikatif dengan jumlah populasi 127 karyawan dan menggunakan sampel 56 responden. Teknik pengumpulan data yang digunakan adalah observasi, wawancara, dan penyebaran kuisioner. Metode analisis yang digunakan adalah analisis regresi linier berganda dan koefisien determinasi. Hasil penelitian menunjukan bahwa komitmen organisasi, lingkungan kerja dan kinerja karyawan dapat dikatakan kurang baik. Komitmen organisasi dan lingkungan kerja memberikan pengaruh terhadap kinerja karyawan sebesar 77,1%. Variabel independen yang dominan adalan lingkungan kerja dengan pengaruh sebesar 51,4% dan komitmen organisasi pengaruh sebesar 25,8%. Kata Kunci : Komitmen Organisasi, Lingkungan Kerja dan Kinerja Karyawa

In Vitro Reassortment between Endemic H1N2 and 2009 H1N1 Pandemic Swine Influenza Viruses Generates Attenuated Viruses

The pandemic H1N1 (pH1N1) influenza virus was first reported in humans in the spring of 2009 and soon thereafter was identified in numerous species, including swine. Reassortant viruses, presumably arising from the co-infection of pH1N1 and endemic swine influenza virus (SIV), were subsequently identified from diagnostic samples collected from swine. In this study, co-infection of swine testicle (ST) cells with swine-derived endemic H1N2 (MN745) and pH1N1 (MN432) yielded two reassortant H1N2 viruses (R1 and R2), both possessing a matrix gene derived from pH1N1. In ST cells, the reassortant viruses had growth kinetics similar to the parental H1N2 virus and reached titers approximately 2 log10 TCID50/mL higher than the pH1N1 virus, while in A549 cells these viruses had similar growth kinetics. Intranasal challenge of pigs with H1N2, pH1N1, R1 or R2 found that all viruses were capable of infecting and transmitting between direct contact pigs as measured by real time reverse transcription PCR of nasal swabs. Lung samples were also PCR-positive for all challenge groups and influenza-associated microscopic lesions were detected by histology. Interestingly, infectious virus was detected in lung samples for pigs challenged with the parental H1N2 and pH1N1 at levels significantly higher than either reassortant virus despite similar levels of viral RNA. Results of our experiment suggested that the reassortant viruses generated through in vitro cell culture system were attenuated without gaining any selective growth advantage in pigs over the parental lineages. Thus, reassortant influenza viruses described in this study may provide a good system to study genetic basis of the attenuation and its mechanism

Influenza H5 Hemagglutinin DNA Primes the Antibody Response Elicited by the Live Attenuated Influenza A/Vietnam/1203/2004 Vaccine in Ferrets

Priming immunization plays a key role in protecting individuals or populations to influenza viruses that are novel to humans. To identify the most promising vaccine priming strategy, we have evaluated different prime-boost regimens using inactivated, DNA and live attenuated vaccines in ferrets. Live attenuated influenza A/Vietnam/1203/2004 (H5N1) candidate vaccine (LAIV, VN04 ca) primed ferrets efficiently while inactivated H5N1 vaccine could not prime the immune response in seronegative ferrets unless an adjuvant was used. However, the H5 HA DNA vaccine alone was as successful as an adjuvanted inactivated VN04 vaccine in priming the immune response to VN04 ca virus. The serum antibody titers of ferrets primed with H5 HA DNA followed by intranasal vaccination of VN04 ca virus were comparable to that induced by two doses of VN04 ca virus. Both LAIV-LAIV and DNA-LAIV vaccine regimens could induce antibody responses that cross-neutralized antigenically distinct H5N1 virus isolates including A/HongKong/213/2003 (HK03) and prevented nasal infection of HK03 vaccine virus. Thus, H5 HA DNA vaccination may offer an alternative option for pandemic preparedness

Characterization of an Artificial Swine-Origin Influenza Virus with the Same Gene Combination as H1N1/2009 Virus: A Genesis Clue of Pandemic Strain

Pandemic H1N1/2009 influenza virus, derived from a reassortment of avian, human, and swine influenza viruses, possesses a unique gene segment combination that had not been detected previously in animal and human populations. Whether such a gene combination could result in the pathogenicity and transmission as H1N1/2009 virus remains unclear. In the present study, we used reverse genetics to construct a reassortant virus (rH1N1) with the same gene combination as H1N1/2009 virus (NA and M genes from a Eurasian avian-like H1N1 swine virus and another six genes from a North American triple-reassortant H1N2 swine virus). Characterization of rH1N1 in mice showed that this virus had higher replicability and pathogenicity than those of the seasonal human H1N1 and Eurasian avian-like swine H1N1 viruses, but was similar to the H1N1/2009 and triple-reassortant H1N2 viruses. Experiments performed on guinea pigs showed that rH1N1 was not transmissible, whereas pandemic H1N1/2009 displayed efficient transmissibility. To further determine which gene segment played a key role in transmissibility, we constructed a series of reassortants derived from rH1N1 and H1N1/2009 viruses. Direct contact transmission studies demonstrated that the HA and NS genes contributed to the transmission of H1N1/2009 virus. Second, the HA gene of H1N1/2009 virus, when combined with the H1N1/2009 NA gene, conferred efficient contact transmission among guinea pigs. The present results reveal that not only gene segment reassortment but also amino acid mutation were needed for the generation of the pandemic influenza virus