159 research outputs found

    Assay of genetic diversity of cuttlefish (Sepia pharaonis; Ehrenberg, 1831) populations using microsatellite markers in Bandar Abbas and Bushehr regions

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    In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology laboratory of Khorramshahr University of Marine Science and Technology. Genomic DNA was extracted with CTAB method. The quality and quantity of extracted DNA was assessed by 1% agarose gel electrophoresis and spectrophotometry, respectively. Polymerase chain reaction conducted with 6 pairs of microsatellite primers. PCR products were electrophoresed on 8% polyacrylamide gel and stained with silver nitrate. These primers were shown 4 pairs of polymorph and 2 pairs of monomorp. Allele Sizes were measured in populations then genetic parameter were calculated using Arlequin and Gen Alex Programs and phylogenetic relationship was determinated and drawn using TFPGA Program. Result obtained showed genetic distance and resemblance distance is 0.282 and 0.754, respectively and Weak but significant genetic differentiation was present 0.031 between of populations

    From embodiment to metaphor: A study on social cognitive development and conceptual metaphor in Persian-speaking children

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    This study explores the metaphoric comprehension of normal Persian-speaking children, as well as theories of cognitive development and cultural and social impacts. The researchers discuss the improvement of the understanding of ontological conceptual metaphors through age growth and cognitive development, and how it helps to expand children�s thoughts and knowledge of the world. In this study, 121 normal native Persian-speaking children from the age of 5 to 13 with no language and cognitive disorders participated. Pearson correlation and one-way ANOVA were used to examine the relationships between pairs of variables. The results showed that children start to comprehend abstract concepts and primary ontological metaphors at about 5 years of age, which is in contrast with what Piaget has implied. Children�s metaphorical comprehension improved progressively with age, social, and cognitive development as other studies have also implied, and they understood more complex types of metaphors by age growth. © 2020 IJSCL. All rights reserved

    Runaway electron energy measurement using hard x-ray spectroscopy in "Damavand" tokamak

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    Set of experiments has been developed to study existing runaway electrons in "Damavand" tokamak plasma upon characteristics of hard x-ray emissions produced by collision of the runaway electrons with the plasma particles and limiters. As a first step, spatial distribution of hard x-ray emissions on the equatorial plane of the torus was considered. Obtained spectra of hard x-ray emissions for different alignments of shielded detector indicate isotropic emissivity in the equatorial plane. This is in agreement with wide angle cone of bremsstrahlung radiations, deduced from the mean value of energy of the runaway electrons. The mean energy was calculated from the slope of the energy spectrum of hard x-ray photons. In the second stage in order to investigate time evolution of energy of the runaway electrons, similar technique were applied to obtain hard x-ray energy in every 3 ms intervals, from the beginning to the end of plasma. The mean energy of the runaway electrons increases during the ramp up phase and reaches its maximum between 3 and 9 ms after plasma formation. Also considering the time dependence of the counted photons in each energy range shows that energetic photons are emitted during the ramp up phase of the plasma current in Damavand tokamak

    Structuring communities for sharing human digital memories in a social P2P network

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    A community is sub-network inside P2P networks that partition the network into groups of similar peers to improve performance by reducing network traffic and high search query success rate. Large communities are common in online social networks than traditional file-sharing P2P networks because many people capture huge amounts of data through their lives. This increases the number of hosts bearing similar data in the network and hence increases the size of communities. This article presents a Memory Thread-based Communities for our Entity-based social P2P network that partition the network into groups of peers sharing data belonging to an entity–person, place, object or interest, having its own digital memory or be a part another memory. These connected peers having further similarities by organizing the network using linear orderings. A Memory-Thread is the collection of digital memories having a common reference key and organized according to some form of correlation. The simulation results show an increase in network performance for the proposed scheme along with a decrease in network overhead and higher query success rate compared to other similar schemes. The network maintains its performance even while the network traffic and size increase

    Synthetic long non-coding RNAs [SINEUPs] rescue defective gene expression in vivo

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    Non-coding RNAs provide additional regulatory layers to gene expression as well as the potential to being exploited as therapeutic tools. Non-coding RNA-based therapeutic approaches have been attempted in dominant diseases, however their use for treatment of genetic diseases caused by insufficient gene dosage is currently more challenging. SINEUPs are long antisense non-coding RNAs that up-regulate translation in mammalian cells in a gene-specific manner, although, so far evidence of SINEUP efficacy has only been demonstrated in in vitro systems. We now show that synthetic SINEUPs effectively and specifically increase protein levels of a gene of interest in vivo. We demonstrated that SINEUPs rescue haploinsufficient gene dosage in a medakafish model of a human disorder leading to amelioration of the disease phenotype. Our results demonstrate that SINEUPs act through mechanisms conserved among vertebrates and that SINEUP technology can be successfully applied in vivo as a new research and therapeutic tool for gene-specific up-regulation of endogenous functional proteins

    Association of food allergies, cow�s milk allergy, and asthma with pediatric inflammatory bowel disease

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    There are controversies on the association of childhood allergic diseases with inflammatory bowel diseases (IBD). The aim of this study was to examine the association between food allergy, cow�s milk allergy (CMA), and asthma with pediatric IBD in Iranian population. This case-control study was conducted on 200 individuals less than 18-year-old (100 with IBD and 100 as control group). Medical records, clinical presentation, and laboratory and para-clinical findings related to food allergy, CMA, and asthma were reviewed for all participants in both groups and were recorded. Among 100 children with IBD, 40 had Crohn's disease, and 60 had ulcerative colitis. The frequency of food allergy, cow's milk allergy, and asthma in children with IBD was significantly higher than the control group (P<0.001). Asthma in children with Crohn's disease was significantly more prevalent than children with ulcerative colitis (P=0.008). Food allergy (OR: 22.1, 95 CI: 5.1-95.05, P<0.001), CMA (OR: 15, 95 CI: 3-67, P<0.001), and asthma (OR: 10, 95 CI: 3-37.05, P<0.001) were significantly associated with increased risk of IBD in children. Food allergy, CMA in infancy and asthma are more prevalent in children with different subtypes of IBD. The diagnosis of these risk factors is associated with increased risk of Crohn's disease and ulcerative colitis. © 2018 Tehran University of Medical Sciences. All rights reserved

    Association of food allergies, cow�s milk allergy, and asthma with pediatric inflammatory bowel disease

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    There are controversies on the association of childhood allergic diseases with inflammatory bowel diseases (IBD). The aim of this study was to examine the association between food allergy, cow�s milk allergy (CMA), and asthma with pediatric IBD in Iranian population. This case-control study was conducted on 200 individuals less than 18-year-old (100 with IBD and 100 as control group). Medical records, clinical presentation, and laboratory and para-clinical findings related to food allergy, CMA, and asthma were reviewed for all participants in both groups and were recorded. Among 100 children with IBD, 40 had Crohn's disease, and 60 had ulcerative colitis. The frequency of food allergy, cow's milk allergy, and asthma in children with IBD was significantly higher than the control group (P<0.001). Asthma in children with Crohn's disease was significantly more prevalent than children with ulcerative colitis (P=0.008). Food allergy (OR: 22.1, 95 CI: 5.1-95.05, P<0.001), CMA (OR: 15, 95 CI: 3-67, P<0.001), and asthma (OR: 10, 95 CI: 3-37.05, P<0.001) were significantly associated with increased risk of IBD in children. Food allergy, CMA in infancy and asthma are more prevalent in children with different subtypes of IBD. The diagnosis of these risk factors is associated with increased risk of Crohn's disease and ulcerative colitis. © 2018 Tehran University of Medical Sciences. All rights reserved
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