595 research outputs found
Bayesian hierarchical EMAX model for doseâ response in early phase efficacy clinical trials
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149669/1/sim8167_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149669/2/sim8167.pd
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Reduced wood stiffness and strength, and altered stem form, in young antisense 4CL transgenic poplars with reduced lignin contents
• Reduced lignin content in perennial crops has been sought as a means to
improve biomass processability for paper and biofuels production, but it is unclear
how this could affect wood properties and tree form.
• Here, we studied a nontransgenic control and 14 transgenic events containing
an antisense 4-coumarate:coenzyme A ligase (4CL) to discern the consequences of
lignin reduction in poplar (Populus sp.). During the second year of growth, trees
were grown either free-standing in a field trial or affixed to stakes in a glasshouse.
• Reductions in lignin of up to 40% gave comparable losses in wood strength and
stiffness. This occurred despite the fact that low-lignin trees had a similar wood
density and up to three-fold more tension wood. In free-standing and staked trees,
the control line had twice the height for a given diameter as did low-lignin trees.
Staked trees had twice the height for a given diameter as free-standing trees in the
field, but did not differ in wood stiffness.
• Variation in tree morphogenesis appears to be governed by lignin · environment
interactions mediated by stresses exerted on developing cells. Therefore our
results underline the importance of field studies for assessing the performance of
transgenic trees with modified wood properties.Keywords: tension wood, lignin, stem form, wood strength, buckling safety factor, transgenic poplar, wood stiffnes
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Tyloses and phenolic deposits in xylem vessels impede water transport in low-lignin transgenic poplars: a study by cryo-fluorescence microscopy
Of 14 transgenic poplar genotypes (Populus tremula × Populus alba) with antisense 4-coumarate:coenzyme A ligase that were grown in the field for 2 years, five that had substantial lignin reductions also had greatly reduced xylem-specific conductivity compared with that of control trees and those transgenic events with small reductions in lignin. For the two events with the lowest xylem lignin contents (greater than 40% reduction), we used light microscopy methods and acid fuchsin dye ascent studies to clarify what caused their reduced transport efficiency. A novel protocol involving dye stabilization and cryo-fluorescence microscopy enabled us to visualize the dye at the cellular level and to identify water-conducting pathways in the xylem. Cryo-fixed branch segments were planed in the frozen state on a sliding cryo-microtome and observed with an epifluorescence microscope equipped with a cryo-stage. We could then distinguish clearly between phenolic-occluded vessels, conductive (stain-filled) vessels, and nonconductive (water- or gas-filled) vessels. Low-lignin trees contained areas of nonconductive, brown xylem with patches of collapsed cells and patches of noncollapsed cells filled with phenolics. In contrast, phenolics and nonconductive vessels were rarely observed in normal colored wood of the low-lignin events. The results of cryo-fluorescence light microscopy were supported by observations with a confocal microscope after freeze drying of cryo-planed samples. Moreover, after extraction of the phenolics, confocal microscopy revealed that many of the vessels in the nonconductive xylem were blocked with tyloses. We conclude that reduced transport efficiency of the transgenic low-lignin xylem was largely caused by blockages from tyloses and phenolic deposits within vessels rather than by xylem collapse
Effect of salinity on water relations of wild barley plants differing in salt tolerance
Root hydraulic conductivity was decreased by salinity in barley plants in parallel with slower transpiration rates and a down-regulation of aquaporin expression in the roots. The effects were larger and faster in a more salinity-tolerant line
Use of the Resection Map system as guidance during hepatectomy
Industrial DesignIndustrial Design Engineerin
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