Non-redundant functions of H2A.Z.1 and H2A.Z.2 in chromosome segregation and cell cycle progression.

© 2021 The Authors. H2A.Z is a H2A-type histone variant essential for many aspects of cell biology, ranging from gene expression to genome stability. From deuterostomes, H2A.Z evolved into two paralogues, H2A.Z.1 and H2A.Z.2, that differ by only three amino acids and are encoded by different genes (H2AFZ and H2AFV, respectively). Despite the importance of this histone variant in development and cellular homeostasis, very little is known about the individual functions of each paralogue in mammals. Here, we have investigated the distinct roles of the two paralogues in cell cycle regulation and unveiled non-redundant functions for H2A.Z.1 and H2A.Z.2 in cell division. Our findings show that H2A.Z.1 regulates the expression of cell cycle genes such as Myc and Ki-67 and its depletion leads to a G1 arrest and cellular senescence. On the contrary, H2A.Z.2, in a transcription-independent manner, is essential for centromere integrity and sister chromatid cohesion regulation, thus playing a key role in chromosome segregation.The Wellcone Trust. Grant Number: 210742/Z/18/Z; Kidscan Children's Cancer Research (Kidscan

Ethnic profiling in the Netherlands? A reflection on expanding preventive powers, ethnic profiling and a changing social and political context

Criminal Justice: Legitimacy, accountability, and effectivit

Tjerita Rossinna

Cover contains Swatoe tjerita jang amat bagoes dan betoel soeda kedjadian di Betawi. Ini tjerita baik di boeat toeladan pada sekalian orang jang berhati kedjem

Tjerita Rossinna

Cover contains Swatoe tjerita jang amat bagoes dan betoel soeda kedjadian di Betawi. Ini tjerita baik di boeat toeladan pada sekalian orang jang berhati kedjem

The ROF+ methodology for grease life testing

Very often, the service life of grease lubricated rolling bearings is determined by the so called “grease life”. This can be tested on R0F test rigs, which have been available for this since more than 40 years. Recently, this technology has been updated and is now called R0F+. The R0F+ can be used as a reference for grease life/relubrication intervals or as a research tool. In this paper a description of the test rig and the test methodology will be given. Also the reliability of the rig will be described by means of a comparison of six test rigs. The test philosophy will be illustrated using the so-called “traffic light” and the “grease performance factor” concepts. The R0F+ goes beyond “standard grease life testing”. The rig is a most valuable tool in grease lubrication research due to its flexibility in varying load, speed, temperature and bearing type. This will be illustrated with some examples

Expansion of off-site percutaneous coronary intervention centres significantly reduces ambulance driving time to primary PCI in the Netherlands

INTRODUCTION: In patients with ST-elevation myocardial infarction (STEMI), percutaneous coronary intervention (PCI)-mediated reperfusion is preferred over pharmacoinvasive reperfusion with fibrinolysis if transfer to a PCI centre can be ensured in ≤120 min. We evaluated the ambulance driving time to primary PCI centres in the Netherlands and assessed to what extent ambulance driving times were impacted by the expansion of off-site PCI centres. METHODS AND RESULTS: We calculated the driving routes from every Dutch postal code to the nearest PCI centre with (on-site) or without (off-site) surgical back-up. We used data from ambulance records to estimate the ambulance driving time on each route. There were 16 on-site and 14 off-site PCI centres. The median (interquartile range) time to on-site PCI centres was 18.8 min (12.2-26.3) compared with 14.9 min (8.9-20.9) to any PCI centre (p 120 min were only seen in non-mainland areas. CONCLUSION: Based on a computational model, timely ambulance transfer to a PCI centre within 120 min is available to almost all STEMI patients in the Netherlands. Expansion of off-site PCI has significantly reduced the driving time to PCI centres