318 research outputs found
Binding of small interfering RNA molecules is crucial for RNA interference suppressor activity of rice hoja blanca virus NS3 in plants
The NS3 protein of rice hoja blanca tenuivirus represents a viral suppressor of RNAi that sequesters small interfering (si)RNAs in vitro. To determine whether this siRNA binding property is the critical determinant for the suppressor activity of NS3, an alanine point mutational analysis was performed and the resulting mutant proteins were tested for both siRNA binding ability and RNAi suppressor activity in plants. Alanine substitutions of lysine residues at position 173-175 resulted in mutant proteins that lost both their affinity for siRNAs and their RNAi suppressor activity in planta. This indicates that siRNA binding of NS3 is indeed essential for the suppressor function of NS3 and that residues at position 173-175 are involved in the siRNA binding and suppressor activit
The influence of additives on the current carrying capacity of bulk YBa2Cu3OX
We studied the influence of using different powders and several additives on the shaping process and critical current density of sintered bulk YBa2Cu3OX. It was found that all additives used facilitated the shaping process. Silver and most organic additives influenced the critical current density only little, Zinc reduced it to zero, but an ethylenebisstearoylamide compound doubled the critical current density as compared to samples without additives
First principles modelling of magnesium titanium hydrides
Mixing Mg with Ti leads to a hydride Mg(x)Ti(1-x)H2 with markedly improved
(de)hydrogenation properties for x < 0.8, as compared to MgH2. Optically, thin
films of Mg(x)Ti(1-x)H2 have a black appearance, which is remarkable for a
hydride material. In this paper we study the structure and stability of
Mg(x)Ti(1-x)H2, x= 0-1 by first-principles calculations at the level of density
functional theory. We give evidence for a fluorite to rutile phase transition
at a critical composition x(c)= 0.8-0.9, which correlates with the
experimentally observed sharp decrease in (de)hydrogenation rates at this
composition. The densities of states of Mg(x)Ti(1-x)H2 have a peak at the Fermi
level, composed of Ti d states. Disorder in the positions of the Ti atoms
easily destroys the metallic plasma, however, which suppresses the optical
reflection. Interband transitions result in a featureless optical absorption
over a large energy range, causing the black appearance of Mg(x)Ti(1-x)H2.Comment: 22 pages, 9 figures, 4 table
Noise properties of direct current SQUIDs with quasiplanar YBa2Cu3O7 Josephson junctions
We describe the noise performance of dc SQUIDs fabricated with quasiplanar rampâtype Josephson junctions on the basis of câaxisâoriented YBa2Cu3O7/PrBa2Cu3O7 thinâfilm heterostructures. The noise spectrum of the dc SQUIDs was measured with dcâ and acâbias schemes at different temperatures and showed values below 10â5 Ί0/Hz1/2 down to frequencies of about 1 Hz at 70 K. Up to now for the magnetic fluxnoise and the energy resolution obtained at 1 kHz and 77 K the best values were 2.5Ă10â6, Ί0/Hz1/2 and 3Ă10â31 J/Hz, respectively. A study of the white and 1/fnoises of the SQUIDs was performed. The influence of magnetic flux, bias current, high static magnetic fields, and aging on the SQUID noise were investigated. The junctions and devices do not degrade due to aging in air or thermal cycling
Using differential reinforcement of high rates of behavior to improve work productivity : a replication and extension
Background: Due to deficits in adaptive and cognitive functioning, productivity may pose challenges for individuals with intellectual disability in the workplace.Method: Using a changingâcriterion embedded in a multiple baseline across particiâpants design, we examined the effects of differential reinforcement of high rates of behaviour (DRH) on the rate of data entry (i.e., productivity) in four adults with intelâlectual disability.Results: Although the DRH procedure increased the rate of correct data entry in all four participants, none of the participants achieved the criterion that we set with novice undergraduate students.Conclusions: Our results indicate that DRH is an effective intervention to increase rate of correct responding in individuals with intellectual disability, but that achievâing the same productivity as workers without disability may not always be possible
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