Short term effects of irradiance on the growth of Pterocladiella capillacea (Gelidiales, Rhodophyta)

Pterocladiella capillacea has been economically exploited for agar extraction in the Azores for many years. Harvesting dropped to a full stop in the early 1990s due to a population collapse, but restarted in 2013. Since then it has been intensively harvested and overexploitation must be prevented, with both sustainable harvesting and effective cultivation practices. This study represents the first attempt to determine optimal conditions for P. capillacea production in the Azores, and evaluates its vegetative growth in two experiments using von Stosch’s medium designed to test entire thallus and tips portions response to different irradiances (30, 70 and 150 μmol photons m¯² s¯¹). The best relative growth rate (RGR) was recorded at 150 μmol photons m¯² s¯¹ for the entire thalli and tips after two-weeks and three-weeks, respectively, indicating that an acclimation period is necessary to assure the growth of this alga under experimental conditions. Higher RGR was obtained at higher irradiance (3.98 ± 2.10% fm day¯¹), but overall, growth rates were low or negative. Epiphytes were a serious problem towards the end of the entire thallus experiments, where Feldmannia irregularis proliferate at all irradiances. Future cultivation approaches complemented with other relevant environmental factors (e.g. pH, photoperiod, salinity), are recommended.FCT – Fundação para a Ciência e Tecnologia projects UID/BIA/00329/2013, 2015 - 2018 and UID/BIA/00329/2019, CIRN (Centro de Investigação de Recursos Naturais, University of the Azores), and CIIMAR (Interdisciplinary Centre of Marine and Environmental Research, Porto, Portugal). RFP was supported by a doctoral grant M3.1.2/F/024/2011, Fundo Regional para a Ciência e Tecnologia.info:eu-repo/semantics/publishedVersio

Opportunities for Seaweed Aquaculture Development in the Azores

Jornadas "Ciência nos Açores – que futuro? Tema Ciências Naturais e Ambiente", Ponta Delgada, 7-8 de Junho de 2013.As macroalgas marinhas têm sido largamente usadas para diversos fins. Nos Açores, várias espécies têm sido usadas tradicionalmente na alimentação humana (e.g. Fucus spiralis, Porphyra spp., Laurencia spp. e Osmundea spp.) e para extracção de compostos com interesse na indústria dos ficocolóides (Pterocladiella capilacea e Gelidium spp.). As exigências no controlo da qualidade e as práticas actuais de colheita de macroalgas marinhas selvagens na Europa levantam preocupações ambientais sérias que tornam premente a necessidade se implementarem métodos de produção de biomassa controlados, como é o caso da aquacultura de macroalgas marinhas. Apesar da importância da exploração sustentável dos recursos marinhos existentes nos Açores, não existe informação sobre a viabilidade do cultivo de macroalgas marinhas no Arquipélago. O conhecimento sobre os requisitos básicos para o cultivo em grande escala das espécies nativas seleccionadas e os locais mais apropriados para a sua implementação está em falta. O objectivo principal do presente projecto é avaliar o potencial de cultivo de espécies de macroalgas marinhas seleccionadas, bem como identificar os métodos de cultivo mais adequados. Os resultados do programa de doutoramento serão de extrema importância quer em termos científicos quer em termos empresariais. Permitirão a transferência de tecnologia para o tecido empresarial regional e para a implementação de empresas de base tecnológica indo ao encontro das futuras políticas de financiamento europeias no âmbito do Programa Europeu Horizonte 2020.ABSTRACT: Seaweeds have a wide range of applications. In the Azores, several species of seaweeds were traditionally used either as food (e.g. Fucus spiralis, Porphyra spp., Laurencia spp. and Osmundea spp.) or for extraction of chemical products (Pterocladiella capilacea e Gelidium spp.). The product quality control requirements and concerns regarding the environmental sustainability of current wild seaweed biomass harvesting practices in Europe demand for controlled seaweed aquaculture. Despite the interest in exploiting Azorean seaweed resources, there is no information on the feasibility of cultivating seaweed in the Azores. Basic knowledge on large scale cultivation requirements of the selected native species is missing. The present project it’s aimed at evaluating the culture potential of selected Azorean species. The resulting outputs will be extremely important for both academic and economic purposes, bringing together the research and the market. Innovative enterprise will benefit from this project and develop technological breakthroughs into viable products with real commercial potential. This main objective is in according with the principal strategy of the Horizon 2020 that will tackle societal challenges after the end of FP7.Fundação para a Ciência e Tecnologi

Rapid testing leads to the underestimation of the scrapie prevalence in an affected sheep and goat flock

To obtain a more detailed understanding of the prevalence of classical scrapie infections in a heavily affected German sheep flock (composed of 603 sheep and 6 goats), we analysed 169 sheep and 6 goats that carried the genotypes susceptible to the disease and that were therefore culled following discovery of the index case. The initial tests were performed using the Biorad TeSeE ELISA and reactive results were verified by official confirmatory methods (OIE-immunoblot and/or immunohistochemistry (IHC)) to demonstrate the deposition of scrapie-associated PrPSc in the brain stem (obex). This approach led to the discovery of 40 additional subclinically scrapie-infected sheep. Furthermore, peripheral lymphatic and nervous tissue samples of the 129 sheep and 6 goats with a negative CNS result were examined by IHC in order to identify any preclinical infections which had not already spread to the central nervous system (CNS). Using this approach we found 13 additional sheep with PrPSc depositions in the gut-associated lymph nodes (GALT) as well as in the enteric nervous system. Moreover, in most of these cases PrPSc was also deposited in the spleen and in the retropharyngeal and superficial cervical lymph nodes. Taken together, these results show a 30.3% infection prevalence in this scrapie-affected flock. Almost 7.4% of the infected animals harboured PrPSc exclusively in the peripheral lymphatic and nervous tissue and were therefore missed by the currently used testing strategy

Проблемы теневой экономики в Украине

Brain inflammation plays a central role in multiple sclerosis (MS). Besides lymphocytes, the astroglia and microglia mainly contribute to the cellular composition of the inflammatory infiltrate in MS lesions. Several studies were able to demonstrate that cortical lesions are characterized by lower levels of inflammatory cells among activated microglia/macrophages. The underlying mechanisms for this difference, however, remain to be clarified. In the current study, we compared the kinetics and extent of microglia and astrocyte activation during early and late cuprizone-induced demyelination in the white matter tract corpus callosum and the telencephalic gray matter. Cellular parameters were related to the expression profiles of the chemokines Ccl2 and Ccl3. We are clearly able to demonstrate that both regions are characterized by early oligodendrocyte stress/apoptosis with concomitant microglia activation and delayed astrocytosis. The extent of microgliosis/astrocytosis appeared to be greater in the subcortical white matter tract corpus callosum compared to the gray matter cortex region. The same holds true for the expression of the key chemokines Ccl2 and Ccl3. The current study defines a model to study early microglia activation and to investigate differences in the neuroinflammatory response of white vs. gray matter

Model experiments for selection on resistance to infection in mice and pigs

International audienc

Seabed images from Southern Ocean shelf regions off the northern Antarctic Peninsula and in the southeastern Weddell Sea

Recent advances in underwater imaging technology allow for the gathering of invaluable scientific information on seafloor ecosystems, such as direct in situ views of seabed habitats and quantitative data on the composition, diversity, abundance, and distribution of epibenthic fauna. The imaging approach has been extensively used within the research project DynAMo (Dynamics of Antarctic Marine Shelf Ecosystems) at the Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research Bremerhaven (AWI), which aimed to comparatively assess the pace and quality of the dynamics of Southern Ocean benthos. Within this framework, epibenthic spatial distribution patterns have been comparatively investigated in two regions in the Atlantic sector of the Southern Ocean: the shelf areas off the northern tip of the Antarctic Peninsula, representing a region with above-average warming of surface waters and sea-ice reduction, and the shelves of the eastern Weddell Sea as an example of a stable high-Antarctic marine environment that is not (yet) affected by climate change. The AWI Ocean Floor Observation System (OFOS) was used to collect seabed imagery during two cruises of the German research vessel Polarstern, ANT-XXIX/3 (PS81) to the Antarctic Peninsula from January to March 2013 and ANT-XXXI/2 (PS96) to the Weddell Sea from December 2015 to February 2016. Here, we report on the image and data collections gathered during these cruises. During PS81, OFOS was successfully deployed at a total of 31 stations at water depths between 29 and 784 m. At most stations, series of 500 to 530 pictures ( >  15 000 in total, each depicting a seabed area of approximately 3.45 m2 or 2.3  ×  1.5 m) were taken along transects approximately 3.7 km in length. During PS96, OFOS was used at a total of 13 stations at water depths between 200 and 754 m, yielding series of 110 to 293 photos (2670 in total) along transects 0.9 to 2.6 km in length. All seabed images taken during the two cruises, including metadata, are available from the data publisher PANGAEA via the two persistent identifiers at https://doi.org/10.1594/PANGAEA.872719 (for PS81) and https://doi.org/10.1594/PANGAEA.862097 (for PS96)

Growth responses of Macrocystis pyrifera (Laminariales), Southern Chile, juvenile sporophytes to nutrient limitation

1st Mares Conference on Marine Ecosystems Health and Conservation. Olhão, Portugal 17-21 November 2014.Kelp forests represent some of the most conspicuous coastal habitats and today we recognize only one giant kelp species (Macrocystis pyrifera) distributed globally [1, 2]. M. pyrifera is recognized as a perennial kelp species with a low capacity of energy storage, whereas its high productivity is associated the availability of nitrogen from the water column [3]. The relation between M. pyrifera growth and biomass production results from a plastic response of the sporophytes to temporal and spatial variability in nitrogen availability [4, 5]. However, the low storage capacity of giant kelp [6, 7] is clearly disadvantageous during periods of suboptimal environmental conditions; as those that occur seasonally in California and the inland waters of southern Chile. Due to an increased demand for kelp biomass in Chile for the world alginate industry and abalone farming in Chile [8, 9] there is an increased demand of raw material and interest for developing kelp aquaculture technologies [10]. The present study evaluates the effect of different nitrogen availability on the growth and regeneration of juvenile fronds of M. pyrifera sporophytes from southern Chile and explore its consequences for the development of seeding strategies of kelp farming in southern Chile

Measurement of the W-pair Production Cross-section and W Branching Ratios at s\sqrt{s}=205 and 207 GeV

The cross-section for the process e+e-->W+W- was measured with the data sample collected by DELPHI at centre-of-mass energies up to 209 GeV and corresponding to a total integrated luminosity of about 209 pb^-1. The branching ratios of the W decay were also measured; from them the value of |Vcs| was extracted. The results are compared with the most recent calculations in the frame of the Standard Model

Identification of the bulk pairing symmetry in high-temperature superconductors: Evidence for an extended s-wave with eight line nodes

we identify the intrinsic bulk pairing symmetry for both electron and hole-doped cuprates from the existing bulk- and nearly bulk-sensitive experimental results such as magnetic penetration depth, Raman scattering, single-particle tunneling, Andreev reflection, nonlinear Meissner effect, neutron scattering, thermal conductivity, specific heat, and angle-resolved photoemission spectroscopy. These experiments consistently show that the dominant bulk pairing symmetry in hole-doped cuprates is of extended s-wave with eight line nodes, and of anisotropic s-wave in electron-doped cuprates. The proposed pairing symmetries do not contradict some surface- and phase-sensitive experiments which show a predominant d-wave pairing symmetry at the degraded surfaces. We also quantitatively explain the phase-sensitive experiments along the c-axis for both Bi_{2}Sr_{2}CaCu_{2}O_{8+y} and YBa_{2}Cu_{3}O_{7-y}.Comment: 11 pages, 9 figure