Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2016-08-10T12:36:22Z No. of bitstreams: 1 Rubsamen-Waigmann H Standard conditions....pdf: 1168798 bytes, checksum: 8a27aca198229a64007a9bbceb037d23 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2016-08-10T13:12:37Z (GMT) No. of bitstreams: 1 Rubsamen-Waigmann H Standard conditions....pdf: 1168798 bytes, checksum: 8a27aca198229a64007a9bbceb037d23 (MD5)Made available in DSpace on 2016-08-10T13:12:37Z (GMT). No. of bitstreams: 1 Rubsamen-Waigmann H Standard conditions....pdf: 1168798 bytes, checksum: 8a27aca198229a64007a9bbceb037d23 (MD5) Previous issue date: 1994-11Georg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, GermanyGeorg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, GermanyNational Institute of Biological Standards and Control. London, UKLos Alamos National Laboratory. HIV Sequence Database. Los Alamos, New MexicoGeorg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, GermanyNational Institute of Biological Standards and Control. London, UKNational Institute of Biological Standards and Control. London, UKFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitute of Tropical Medicine. Department of Microbiology. Antwerp, BelgiumUganda Virus Research Institute. Entebbe, UgandaMahidol University. Faculty of Medicine Siriraj Hospital. Department of Microbiology, Division of Virology. Bangkok, ThailandWorld Health Organization. Global Programme on AIDS. Geneva, SwitzerlandLos Alamos National Laboratory. HIV Sequence Database. Los Alamos, New MexicoWHO Network for HIV Isolation and CharacterizationHIV-1 isolates were obtained from four countries within the framework of the WHO Network for HIV Isolation and Characterization. The use of standard HIV isolation procedures allowed us to compare the biological properties of 126 HIV-1 isolates spanning five genetic subtypes. In primary isolation cultures, viruses from Uganda and Brazil appeared early and replicated without delay, whereas the replication of Thai viruses was delayed by several weeks. Regardless of genetic subtype or country of origin, blood samples collected more than 2 years after seroconversion yielded virus that replicated efficiently in the primary isolation cultures. None of the isolates obtained from Thailand or Rwanda replicated in cell lines, whereas 5 of the 13 Brazilian isolates and 7 of the 11 Ugandan isolates replicated and induced syncytia in Ml -2 cells. As expected for virus isolates obtained early in HIV-1 infection (within 2 years of seroconversion), all viruses from Brazil, Rwanda, and Thailand showed a slow/low replicative pattern. For the Ugandan samples, the time from seroconversion was known precisely for a few of the samples and only in one case was less than 2 years. This may explain why the five viruses that were able to replicate in all cell lines, and thus classified as rapid/high, were of Ugandan origin. Viruses able to induce syncytia in MT-2 cells, also induced syncytia in PBMC. However, 8 slow/low viruses (out of 27) gave discordant results, inducing syncytia in PBMC but not in MT-2 cells. Furthermore, using syncytium induction as a marker, changes in virus populations during early in vitro passage in PBMC could be observed. The results indicate that biological variation is a general property of HIV-1 in different regions of the world. Moreover, the time from HIV-1 infection, rather than genetic subtype, seems to be linked to viral phenotype