57 research outputs found

    Medidas da transpiração em porta-enxertos de citros

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    Estudou-se a variação diurna da transpiração de quatro porta -enxertos de citros, durante 12 horas, em intervalos de 60 minutos, pelo método das pesagens. Verificou-se que não ocorreu diferenças estatísticas na marcha diária da transpiração, entre Poncirus trifoliata, Citrus aurantium (laranja Azeda), Citrus sinensis (laranja Caipira) e Citrus limonia (limão Cravo). O limoeiro Cravo mostrou, nas condições estudadas, área foliar superior à Poncirus, laranja Azeda e laranja Caipira. Observou-se ainda uma correlação positiva entre a área foliar e a transpiração dos quatro porta-enxertos de citros.Diurnal transpiration rates of four citrus rootstocks were measured during 12 hours separated by intervals of 60 minutes, through the weighing method. Plants of Poncirus trifoliata, Citrus aurantium, Citrus sinensis, and Citrus limonia do not present statistical differences in the daily march of transpiration. In the studied conditions C. limonia showed higher leaf area than P. trifoliata, C. aurantium, and C. sinensis. There was a positive correlation between leaf area and transpiration of the four citrus rootstodks

    No improvement of survival with reduced- versus high-intensity conditioning for allogeneic stem cell transplants in Ewing tumor patients

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    Background: Outcomes of Ewing tumor (ET) patients treated with allogeneic stem cell transplantation (allo-SCT) were compared regarding the use of reduced-intensity conditioning (RIC) and high-intensity conditioning (HIC) regimens as well as human leukocyte antigen (HLA)-matched and HLA-mismatched grafts. Patients and methods: We retrospectively analyzed data of 87 ET patients from the European Group for Blood and Marrow Transplantation, Pediatric Registry for Stem Cell Transplantations, Asia Pacific Blood and Marrow Transplantation and MetaEICESS registries treated with allo-SCT. Fifty patients received RIC (group A) and 37 patients received HIC (group B). Twenty-four patients received HLA-mismatched grafts and 63 received HLA-matched grafts. Results: Median overall survival was 7.9 months [±1.24, 95% confidence interval (CI) 5.44-10.31] for group A and 4.4 months (±1.06, 95% CI 2.29-6.43) for group B patients (P = 1.3). Death of complications (DOC) occurred in 4 of 50 (0.08) and death of disease (DOD) in 33 of 50 (0.66) group A and in 16 of 37 (0.43) and 17 of 37 (0.46) group B patients, respectively. DOC incidence was decreased (P < 0.01) and DOD/relapse increased (P < 0.01) in group A compared with group B. HLA mismatch was not generally associated with graft-versus-Ewing tumor effect (GvETE). Conclusions: There was no improvement of survival with RIC compared with HIC due to increased DOD/relapse incidence after RIC despite less DOC incidence. This implicates general absence of a clinically relevant GvETE with current protocol

    The Kinetics of Early T and B Cell Immune Recovery after Bone Marrow Transplantation in RAG-2-Deficient SCID Patients

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    The kinetics of T and B cell immune recovery after bone marrow transplantation (BMT) is affected by many pre- and post-transplant factors. Because of the profoundly depleted baseline T and B cell immunity in recombination activating gene 2 (RAG-2)-deficient severe combined immunodeficiency (SCID) patients, some of these factors are eliminated, and the immune recovery after BMT can then be clearly assessed. This process was followed in ten SCID patients in parallel to their associated transplant-related complications. Early peripheral presence of T and B cells was observed in 8 and 4 patients, respectively. The latter correlated with pre-transplant conditioning therapy. Cells from these patients carried mainly signal joint DNA episomes, indicative of newly derived B and T cells. They were present before the normalization of the T cell receptor (TCR) and the B cell receptor (BCR) repertoire. Early presentation of the ordered TCR gene rearrangements after BMT occurred simultaneously, but this pattern was heterogeneous over time, suggesting different and individual thymic recovery processes. Our findings early after transplant could suggest the long-term patients' clinical outcome. Early peripheral presence of newly produced B and T lymphocytes from their production and maturation sites after BMT suggests donor stem cell origin rather than peripheral expansion, and is indicative of successful outcome. Peripheral detection of TCR excision circles and kappa-deleting recombination excision circles in RAG-2-deficient SCID post-BMT are early markers of T and B cell reconstitution, and can be used to monitor outcome and tailor specific therapy for patients undergoing BMT

    Effect of partial soil wetting on transpiration, vegetative growth and root system of young orange trees

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    The wetted area fraction is a factor critical to the success of drip irrigation. This study aimed to evaluate the effect of partial soil wetting on transpiration, vegetative growth and root system of young orange trees. The experiment was carried out in a greenhouse where plants were grown in 0.5 m3boxes internally divided into compartments. The wetting of 12 % of soil area was tested on two types of soil cultivated with ‘Valencia’ orange trees grafted onto Rangpur lime and ‘Swingle’ citrumelo rootstocks. Transpiration was determined in 40 plants. Water extraction and root density were evaluated in the compartments. Transpiration is reduced by restriction in wetted soil area, and such reduction is influenced by the number of days after the beginning of partial irrigation, atmospheric evaporative demand and plant phenological stage. Mean transpiration of plants with partial irrigation was equivalent to 84 % of the mean transpiration of plants with 100 % of wetted soil area in the period studied. However, after 156 days of imposing partial irrigation there was no difference in transpiration between treatments. Plant acclimation was caused by an increase in root concentration in the irrigated area. After a period of acclimation, if the entire root system is wetted, soil water extraction becomes proportional to the percentage of wetted area after a short period of time. Despite the reduction in transpiration, there was no difference between treatments with 12 % and 100 % of wetted soil area in terms of vegetative growth

    Water relations and photosynthesis as criteria for adequate irrigation management in 'Tahiti' lime trees

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    Irrigation scheduling based on soil moisture status is one of the most useful methods because of its practicality and low cost. The effects of available soil water depletion on evapotranspiration (ETc), transpiration (E), leaf water potential at predawn (psiP) and midday (psiM), stomatal conductance (gs) and net CO2 assimilation (A) in lime 'Tahiti' trees (Citrus latifolia) were evaluated to improve irrigation schedule and minimize water use without causing water stress. The trees were spaced 7 <FONT FACE=Symbol>&acute;</FONT> 4 m and drip-irrigated by four drippers with the available soil water content (AWC) depleted by suspension of irrigation (40 days). Leaf water potential was measured on a pressure chamber (psiP and psiM) and leaf gas exchange was measured by infrared gas analyzer (E, gs and A). Evapotranspiration was determined with the aid of weighing lysimeter. Water soil content and potential (psiS) were monitored with TDR probes and tensiometers, respectively, installed at 0.3, 0.6 and 0.9 m depths. Meteorological variables were monitored with an automatic weather station in the experimental area. The threshold AWC level for the onset of ETc decline was 43%, and 60% for gs, A, E and Y P. Also, psiP was more sensitive to AWC than psiM, and is therefore a better tool for irrigation. When AWC was around 60%, values of psiP and psis were -0.62 MPa and -48.8 kPa, respectively

    Transpiration reduction by surface films

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