Restaurierung des »Grünroten Zimmers« bzw. »Ankleidezimmers des Herrn« im Barockschloss Rammenau: Abnahme einer Überfassung mittels Lasertechnologie

Bis Ende 2010 wurde das Herren-Ankleidezimmer im Rammenauer Schloss grundlegend restauriert. Die Restauratoren beschreiben sowohl die erste Farbfassung des Raumes, als auch den Zustand vor 2009 und die Methoden ihres Restaurierungsverfahrens

Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells

<p>Abstract</p> <p>Background</p> <p>Hypoxia plays a critical role in various cellular mechanisms, including proliferation and differentiation of neural stem and progenitor cells. In the present study, we explored the impact of lowered oxygen on the differentiation potential of human neural progenitor cells, and the role of erythropoietin in the differentiation process.</p> <p>Results</p> <p>In this study we demonstrate that differentiation of human fetal neural progenitor cells under hypoxic conditions results in an increased neurogenesis. In addition, expansion and proliferation under lowered oxygen conditions also increased neuronal differentiation, although proliferation rates were not altered compared to normoxic conditions. Erythropoietin partially mimicked these hypoxic effects, as shown by an increase of the metabolic activity during differentiation and protection of differentiated cells from apoptosis.</p> <p>Conclusion</p> <p>These results provide evidence that hypoxia promotes the differentiation of human fetal neural progenitor cells, and identifies the involvement of erythropoietin during differentiation as well as different cellular mechanisms underlying the induction of differentiation mediated by lowered oxygen levels.</p

Glucosylsphingosine Is a Highly Sensitive and Specific Biomarker for Primary Diagnostic and Follow-Up Monitoring in Gaucher Disease in a Non-Jewish, Caucasian Cohort of Gaucher Disease Patients

Gaucher disease (GD) is the most common lysosomal storage disorder (LSD). Based on a deficient β-glucocerebrosidase it leads to an accumulation of glucosylceramide. Standard diagnostic procedures include measurement of enzyme activity, genetic testing as well as analysis of chitotriosidase and CCL18/PARC as biomarkers. Even though chitotriosidase is the most well-established biomarker in GD, it is not specific for GD. Furthermore, it may be false negative in a significant percentage of GD patients due to mutation. Additionally, chitotriosidase reflects the changes in the course of the disease belatedly. This further enhances the need for a reliable biomarker, especially for the monitoring of the disease and the impact of potential treatments.Here, we evaluated the sensitivity and specificity of the previously reported biomarker Glucosylsphingosine with regard to different control groups (healthy control vs. GD carriers vs. other LSDs).Only GD patients displayed elevated levels of Glucosylsphingosine higher than 12 ng/ml whereas the comparison controls groups revealed concentrations below the pathological cut-off, verifying the specificity of Glucosylsphingosine as a biomarker for GD. In addition, we evaluated the biomarker before and during enzyme replacement therapy (ERT) in 19 patients, demonstrating a decrease in Glucosylsphingosine over time with the most pronounced reduction within the first 6 months of ERT. Furthermore, our data reveals a correlation between the medical consequence of specific mutations and Glucosylsphingosine.In summary, Glucosylsphingosine is a very promising, reliable and specific biomarker for GD

Restaurierung des »Grünroten Zimmers« bzw. »Ankleidezimmers des Herrn« im Barockschloss Rammenau: Abnahme einer Überfassung mittels Lasertechnologie

Bis Ende 2010 wurde das Herren-Ankleidezimmer im Rammenauer Schloss grundlegend restauriert. Die Restauratoren beschreiben sowohl die erste Farbfassung des Raumes, als auch den Zustand vor 2009 und die Methoden ihres Restaurierungsverfahrens

Restaurierung des »Grünroten Zimmers« bzw. »Ankleidezimmers des Herrn« im Barockschloss Rammenau: Abnahme einer Überfassung mittels Lasertechnologie

Bis Ende 2010 wurde das Herren-Ankleidezimmer im Rammenauer Schloss grundlegend restauriert. Die Restauratoren beschreiben sowohl die erste Farbfassung des Raumes, als auch den Zustand vor 2009 und die Methoden ihres Restaurierungsverfahrens

Collaborative Factory Layout Planning With Building Information Modeling

Manufacturing companies are facing an increasingly turbulent environment. In order to respond to these dynamic market conditions, products and thus also production systems have to be adapted more frequently and much faster. However, time and cost targets are often missed by classic factory planning approaches due to the lack of interoperability. Therefore, new ways have to be found in factory planning to overcome these problems. Building Information Modeling (BIM), which is used in the construction industry, provides a promising method for collaboration and interaction in complex projects. Therefore, an approach is presented to systematically implement the BIM method in the factory layout planning process. The aim of this approach is to achieve a higher degree of collaboration between the individual specialist planners in earlier planning phases in order to reduce time and costs over the entire project and to lead to better and more valid planning decisions and results. For this purpose, the individual roles in the planning process are defined, a process diagram for layout planning is drawn up, and a basic data set is specified for the systematic entry of the trades

Chemical structure of Glucosylsphingosine.

<p>This substance was only analysed by HPLC-MS/MS with high sensitivity and specificity of diagnosing GD.</p