Hyperpolarized Long-T1 Silicon Nanoparticles for Magnetic Resonance Imaging

Silicon nanoparticles are experimentally investigated as a potential hyperpolarized, targetable MRI imaging agent. Nuclear T_1 times at room temperature for a variety of Si nanoparticles are found to be remarkably long (10^2 to 10^4 s) - roughly consistent with predictions of a core-shell diffusion model - allowing them to be transported, administered and imaged on practical time scales without significant loss of polarization. We also report surface functionalization of Si nanoparticles, comparable to approaches used in other biologically targeted nanoparticle systems.Comment: supporting material here: http://marcuslab.harvard.edu/Aptekar_hyper1_sup.pd

The Transferrin Receptor: A Potential Molecular Imaging Marker for Human Cancer

Noninvasive imaging of differences between the molecular properties of cancer and normal tissue has the potential to enhance the detection of tumors. Because overexpression of endogenous transferrin receptor (TfR) has been qualitatively described for various cancers and is presumably due to malignant transformation of cells, TfR may represent a suitable target for application of molecular imaging technologies to increase detection of smaller tumors. In the work reported here, investigation into the biology of this receptor using electron microscopy has demonstrated that iron oxide particles targeted to TfR are internalized and accumulate in lysosomal vesicles within cells. Biochemical analysis of the interaction of imaging probes with cells overexpressing the TfR demonstrated that the extent of accumulation, and therefore probe efficacy, is dependent on the nature of the chemical cross-link between transferrin and the iron oxide particle. These data were utilized to design and synthesize an improved imaging probe. Experiments demonstrate that the novel magnetic resonance imaging (MRI) probe is sensitive enough to detect small differences in endogenous TfR expression in human cancer cell lines. Quantitative measurement of TfR overexpression in a panel of 27 human breast cancer patients demonstrated that 74% of patient cancer tissues overexpressed the TfR and that the sensitivity of the new imaging agent was suitable to detect TfR overexpression in greater than 40% of these cases. Based on a biochemical and cell biological approach, these studies have resulted in the synthesis and development of an improved MRI probe with the best in vitro and in vivo imaging properties reported to date

ELP-OA: Final report of the feasibility study

International audienceWe report the results of the feasibility study of the polychromatic laser guide star (PLGS) concept to measure the tilt without any natural guide star. Experiments on the sky at the LLNL (Ca) and at CEA/Pierrelatte (France) to measure the return flux in particular at 330nm reasonably fit the CEA Optical Bloch equation model of the population of the relevant enrgy levels of sodiu atoms in the mesosphere. The accuracy of the measurement of the differential tilt to derive the absolute tilt has been estimated from a horizontal propagation experiment. A phase retrieval algorithm has been developed to improve the measurement accuracy from the monochromatic components of the polychromatic image. A pendular seismometer has been developed to measure telescope vibrations. An end-to-end model has been developed to evaluate the performances expected from a PLGS at different telescopes. In conclusion, next steps of the "Etoile Laser Polychromatique pour Optique" programme. Adaptative" are described

Feasibility study of the polychromatic laser guide star

International audienceWe briefly recall the principle of the polychromatic laser guide star, which aims at providing measurements of the tilt of incoming wavefronts with a 100% sky coverage, We describe the main results of the feasibility study of this concept undertaken within the ELP-OA porgramme. We finally summarize our plans for a full demonstrator at Observatoire de Haute-Provence

Writing week-journals to improve the writing quality of fourth-graders' compositions

Students' writing problems are a global educational concern and is in need of particular attention. This study aims to examine the impact of providing extra writing opportunities (i.e., writing journals) on the quality of writing compositions. A longitudinal cluster-randomized controlled design using a multilevel modeling analysis with 182 fourth grade students was conducted. We examined whether students' writing quality differed when writing journals on a weekly basis for 12 weeks, compared with a control group. Three covariates were analyzed, namely: (i) the students' attitudes towards writing; (ii) their self-efficacy in writing; (iii) and their use of self-regulation (SRL) strategies while writing. Findings have shown that students who wrote week-journals significantly improved the writing quality of their compositions and reported a higher use of SRL strategies in writing. Nevertheless, self-efficacy and attitude towards writing were found to not be related to the quality of the compositions. Moreover, data indicated that the writing quality of compositions improved along with the writing quality of the week-journals. Findings suggest the use of week-journals in class to promote writing.(undefined)info:eu-repo/semantics/publishedVersio